We have measured and characterized the thermal crosstalk in two different arrays of transition-edge sensor (TES) Xray micro-calorimeters with frequency-domain multiplexing (FDM) readout. The TES arrays are fabricated at SRON and are a 8×8 and a 32×32 "kilo-pixel" uniform array. The amount of crosstalk is evaluated as the ratio between the averaged crosstalk signal and the X-ray pulse amplitudes. The crosstalk ratios (CR) for our detectors are compliant with the requirements for future X-ray space missions, such as Athena X-IFU (CR< 10 −3 for first-neighbour pixels): we measured a nearest-neighbour thermal crosstalk ratio at a level of 10 −4 , with a highest crosstalk ratio of 4 × 10 −4 for the kilo-pixel array (worst case, center of array) and 1 × 10 −4 for the 8×8 array, with a margin of improvement achievable by optimizing the Cu metallization and the width of the Si supporting structures (muntins) in the backside of the TES array chip. Based on the measured crosstalk ratios, we have estimated the impact on the spectral resolution by means of noise equivalent power (NEP) considerations and a Monte Carlo simulation, finding an average degradation in quadrature of less than 40 meV, compliant with the < 0.2 eV requirement for Athena X-IFU.