High background radiation areas of Ramsar in Iran: evaluation of DNA damage by alkaline single cell gel electrophoresis (SCGE)

Masoomi, J.R.; Mohammadi, Shima; Amini, Mahdi; Ghiassi-Nejad, M.

doi:10.1016/j.jenvrad.2005.08.005

Cited by 27 publications

(12 citation statements)

References 47 publications

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“…The linear increase in the DNA damage indicates that activation of repair enzymes is not triggered by exposure to radiation in HBRA. It is noteworthy that in the previous studies in this HBRA, conducted for human lymphocytes, a very similar high DNA damage was also observed Masoomi et al, 2006). But in the human cases more efficient repair was observed which interpreted to radioadaptation.…”

Section: Discussionsupporting

confidence: 79%

Evaluation of DNA damage in the root cells of Allium cepa seeds growing in soil of high background radiation areas of Ramsar – Iran

Saghirzadeh

Gharaati

Mohammadi

et al. 2008

Journal of Environmental Radioactivity

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Section: Discussionsupporting

confidence: 79%

Evaluation of DNA damage in the root cells of Allium cepa seeds growing in soil of high background radiation areas of Ramsar – Iran

Saghirzadeh

Gharaati

Mohammadi

et al. 2008

Journal of Environmental Radioactivity

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“…), although DNA damage measured by the comet assay has been reported to be considerably greater in lymphocytes of Ramsar inhabitants than in persons exposed to normal background radiation, the repair rate is higher in the former only if exposure to radiation was relatively low (Masoomi et al . ). In Chernobyl, lymphocytes of people chronically exposed to low doses from fallout did not show evidence of radio‐adaptation regarding frequency of chromosome and chromatid aberrations after a challenging γ‐ray irradiation (Padovani et al .…”

Section: Discussionmentioning

confidence: 97%

Chronic exposure to low‐dose radiation atChernobyl favours adaptation to oxidative stress in birds

et al. 2014

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Summary 1.Ionizing radiation produces oxidative stress, but organisms can adapt to their exposure with physiological adaptive responses. However, the role of radioadaptive responses in wild populations remains poorly known. 2. At Chernobyl, studies of birds and other taxa including humans show that chronic exposure to radiation depletes antioxidants and increases oxidative damage. Here, we present analyses of levels of the most important intracellular antioxidant (i.e. glutathione, GSH), its redox status, DNA damage and body condition in 16 species of birds exposed to radiation at Chernobyl. We use an approach that allows considering the individual bird as the sampling unit while controlling for phylogenetic effects, thus increasing the statistical power by avoiding the use of species means as done for most previous comparative studies. 3. As a consequence, we found a pattern radically different from previous studies in wild populations, showing that GSH levels and body condition increased, and oxidative stress and DNA damage decreased, with increasing background radiation. Thus, when several species are considered, the overall pattern indicates that birds are not negatively affected by chronic exposure to radiation and may even obtain beneficial hormetic effects following an adaptive response. Analysis of the phylogenetic signal supports the existence of adaptation in the studied traits, particularly in GSH levels and DNA damage. 4. We also show that, under equal levels of radiation, the birds that produce larger amounts of the pigment pheomelanin and lower amounts of eumelanin pay a cost in terms of decreased GSH levels, increased oxidative stress and DNA damage, and poorer body condition. Radiation, however, diminished another potential cost of pheomelanin, namely its tendency to produce free radicals when exposed to radiation, because it induced a change towards the production of less pro-oxidant forms of pheomelanin with higher benzothiazole-to-benzothiazine ratios, which may have facilitated the acclimation of birds to radiation exposure. 5. Our findings represent the first evidence of adaptation to ionizing radiation in wild animals, and confirm that pheomelanin synthesis represents an evolutionary constraint under stressful environmental conditions because it requires GSH consumption.

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“…The RAR in Takandeang Sub-village inhabitants probably is due to highly efficient DNA repair processes. Masoomi et al in 2006 evaluated the DNA repair in Ramsar inhabitants by comet assay [28]. They found that Ramsar inhabitants who had received a lower chronic dose (ranged from 0.53 to 7.62 mSv/y) showed a lower radiosensitivity and more DNA repair rate compared to control samples.…”

Section: Individual Radiosensitivitymentioning

confidence: 99%

“…Until now, there have not been many studies on the correlation between Arg72Pro and radiosensitivity. Comprehensive studies about the association between TP53 Arg72Pro polymorphism and radiosensitivity were already performed by Alsbeih et al [8,28]. In 2007 Alsbeih et al observed an increase of survival in fibroblast cells using clonogenic assay after exposed to single radiation dose ranging from 0 to 4 Gy in cells containing Proline coding allele in TP53 (Arg72Pro) gene.…”

Section: Association Between Tp53 Arg72pro Polymorphism and Radiosensmentioning

confidence: 99%

Assessment of Individual Radiosensitivity in Inhabitants of Takandeang Village － A High Background Radiation Area in Indonesia

et al. 2019

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People living in high background radiation areas (HBRAs) possibly develop the radioadaptive response (RAR) phenomenon. The Mamuju area in West Sulawesi Indonesia is known as an HBRA in Indonesia due to its high natural uranium contents. It is possible that RAR has developed in Mamuju inhabitants. To prove this hypothesis, here in this study, evaluation of the individual radiosensitivity in the inhabitants of Takandeang Village, Mamuju, was conducted using G 2 micronucleus (MN) assay. Association between blood groups and TP53 Arg72Pro polymorphism with individual radiosensitivity was also evaluated in this study. Using G 2 MN assay, we assessed the individual radiosensitivity of Takandeang Village inhabitants and control samples. For each sample, three parameters were calculated. The spontaneous (baseline) MN number, MN number after 0.5 Gy in vitro irradiation, and radiation-induced MN were calculated to predict the individual radiosensitivity. The radiation-induced MN was defined by subtracting the spontaneous MN number from the MN number after irradiation. The mean and SD of the number of micronuclei induced by radiation found in control group (CG) was set as the cutoff value to determine the individual radiosensitivity in all samples. The occurrence of a radiation-induced MN value higher than the mean CG + 1SD CG was scored as 1, indicating a milder radiosensitive phenotype, whereas a result higher than the mean CG + 2SD CG was scored as 2, and indicated a more severe radiosensitive phenotype. When the individual value was lower than the mean CG + 1SD CG, a score of 0 was attributed to the tested subject. The results showed that four individuals in Takandeang Village inhabitants had a milder radiosensitive phenotype, while the others were categorized as normal radiosensitive. A similar finding was also found in control samples. Our study failed to find any correlation between radiosensitivity and either blood group or the TP53 Arg72Pro polymorphism. Overall, our study revealed the possibility of RAR phenomena in Takandeang Village inhabitants. Further investigation using a different point of radiation dose value and larger sample number should be performed to validate this study results.Journal homepage: http://aij.batan.go.id

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High background radiation areas of Ramsar in Iran: evaluation of DNA damage by alkaline single cell gel electrophoresis (SCGE)

Cited by 27 publications

References 47 publications

Evaluation of DNA damage in the root cells of Allium cepa seeds growing in soil of high background radiation areas of Ramsar – Iran

Evaluation of DNA damage in the root cells of Allium cepa seeds growing in soil of high background radiation areas of Ramsar – Iran

Chronic exposure to low‐dose radiation atChernobyl favours adaptation to oxidative stress in birds

Assessment of Individual Radiosensitivity in Inhabitants of Takandeang Village － A High Background Radiation Area in Indonesia

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