High butter-fat diet and bisphenol A additively impair male rat spermatogenesis

Abstract

Exposure to xenoestrogens is a probable cause of male infertility in humans. Consumption of high-fat diets and exposure to bisphenol A (BPA) is pervasive in America. Here, we test the hypothesis that gestational exposure to high dietary fats and/or BPA disrupt spermatogenesis in adulthood. Sprague-Dawley rats were fed diets containing 10 kcal% butter fat (AIN), 39 kcal% butter fat (HFB), or 39 kcal% olive oil (HFO), with or without BPA (25 μg/kg body weight/day) during pregnancy. One group of male offspring received testosterone (T)- and estradiol-17β (E2)-filled implants or sham-implants from postnatal day (PND)70-210. Another group was naturally aged to 18 months. We found that adult males with gestational exposure to BPA, HFB, or HFB + BPA, in both the aged group and the T + E2-implanted group, exhibited impairment of spermatogenesis. In contrast, gestational exposure to HFO or HFO + BPA did not affect spermatogenesis. Sham-implanted, gestational exposed groups also had normal spermatogenesis. Loss of ERα expression in round spermatids and premature expression of protamine-1 in diplotene spermatocytes were features associated with impaired spermatogenesis. Compared with the single-treatment groups, the HFB + BPA group experienced more severe effects, including atrophy.

Transgenerational effects of maternal bisphenol: a exposure on offspring metabolic health

“…An abnormal increase in insulin secretion in the lower BPA group is likely to eventually lead to exhaustion of 50 This is further supported by data from human and animal studies showing that a constant increase in insulin secretion can lead to mitochondrial dysfunction and endoplasmic reticulum stress, which leads to cellular injury and a progressive decline in β-cell function (i.e., βcell exhaustion). 51,52 Furthermore, the third-generation animals of the current study may display an altered metabolic response, if challenged by a second insult such as a high-fat diet as reported in pancreas 53 and other target tissues like mammary gland, sperm and liver [54][55][56] of the first-generation offspring. This requires further investigation.…”

Data on spermatogenesis in rat males gestationally exposed to bisphenol A and high fat diets

“…1 B and C ). More details on the T+E2 model, tissue collection and data analyses are outlined in Tarapore et al, 2016 [1]. For Figs.…”

“…This data article contains supporting information regarding the research article entitled “High butter-fat diet and bisphenol A additively impair male rat spermatogenesis” (P. Tarapore, M. Hennessy, D. Song, J. Ying, B. Ouyang, V. Govindarajah, et al,) [1]. Sprague–Dawley females were fed AIN, high fat butter, 17α-ethinyl estradiol, or high fat butter plus four bisphenol A doses (2500 µg/kg bw-d, 250 µg/kg bw-d, 25 µg/kg bw-d, and 2.5 µg/kg bw-d) before and during pregnancy.…”

Perfluoroalkyl Chemicals and Male Reproductive Health: Do PFOA and PFOS Increase Risk for Male Infertility?

“…There is very little information on the food/diet fed to these rodents. It has been shown that the fatty acid content of diets could be an important source of experimental variation impacting spermatogenesis [ 23 , 131 , 132 , 133 , 134 , 135 , 136 , 137 , 138 ].…”