High cAMP attenuation of insulin-stimulated meiotic G2-M1 transition in zebrafish oocytes: Interaction between the cAMP-dependent protein kinase (PKA) and the MAPK3/1 pathways

Maitra, Saumen Kumar; Das, Debabrata; Ghosh, Pritha; Hajra, Sudip; Roy, Sib Sankar; Bhattacharya, Samir

doi:10.1016/j.mce.2014.06.008

Cited by 35 publications

(41 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Earlier we have reported that PI3K/Akt pathway is indispensable for insulin-induced meiotic maturation in denuded zebrafish oocyte, where PDE3 might act as possible a down-stream target (Das et al 2013). Although involvement of a cAMP-independent PI3K/Akt signalling cascade has been proposed in starfish, striped bass and Atlantic croaker during MISinduced maturation (Sadler & Ruderman 1998, Weber & Sullivan 2001, Pace & Thomas 2005, high cAMP could block insulin-induced GVBD response in zebrafish oocytes (Maitra et al 2014). Taken together these data suggest that cAMP-mediated signalling might have strong negative influence on both maturational steroid and Igf regulation of meiotic G2-M1 transition in zebrafish oocytes.…”

Section: Discussionmentioning

confidence: 90%

“…Previously, high cAMP has been shown to activate PKA which subsequently block synthesis of cyclin B and resumption of meiosis (Conti et al 2002). Moreover, potential antagonism between cAMP and receptor tyrosine kinase-regulated signalling axes is evident from the fact that high cAMP/PKA could successfully abrogate insulin-mediated MEK/MAPK signalling axis in zebrafish oocytes (Maitra et al 2014). Taking cue from these reports, we reasoned involvement of PKA in the cross-talk between E 2 , MIS and Igfmediated signalling events that may act in tandem to regulate prophase arrest and resumption of meiotic maturation in this species.…”

Section: Discussionmentioning

confidence: 99%

“…The catalytic sub-unit of PKA remain phosphorylated on its activation loop (phospho-PKAc) allowing proper substrate recognition and catalysis (Moore et al 2002). In the present study, endogenous PKA activity was assessed indirectly by immunoblot analysis using p-PKAa/b/g cat (Thr198) antibody , Maitra et al 2014. As shown in Fig.…”

Section: Effect Of Camp/pka Regulation Of Dhp And/or Igf1 Induced Meimentioning

confidence: 99%

“…SQ22536 (SQ; 1-10 mm) was dissolved in DMSO and was added to the culture medium either alone or in dbcAMP (1 mM)-primed oocytes. The doses of Wrt, LY, DEBC, forskolin and dbcAMP used were as described earlier (Das et al 2013, Maitra et al 2014. All chemicals were prepared at 1000-fold stock and control wells received equal volume of solvent only.…”

Section: Oocyte Preparation and In Vitro Culturementioning

confidence: 99%

“…All chemicals were prepared at 1000-fold stock and control wells received equal volume of solvent only. Triton X-100 mediated delivery of anti-IGF-IRb into the oocytes was done according to the method described earlier (Maitra et al 2014). In brief, Triton X-100 was diluted (0.1%, v/v) with 10 mM PBS; pH 7.2 and sonicated for 1 min and allowed to complex with anti-IGF-IRb antibody (20 mg/ml) for 30 min each at 25G1 8C and at 4 8C.…”

Section: Oocyte Preparation and In Vitro Culturementioning

confidence: 99%

See 4 more Smart Citations

Releasing prophase arrest in zebrafish oocyte: synergism between maturational steroid and Igf1

Das¹,

Pal²,

Maitra³

2016

Reproduction

Self Cite

View full text Add to dashboard Cite

Binding of 17b-estradiol (E 2 ) to novel G-protein coupled receptor, Gper1, promotes intra-oocyte adenylyl cyclase activity and transactivates epidermal growth factor receptor to ensure prophase-I arrest. Although involvement of either membrane progestin receptor (mPR) or Igf system has been implicated in regulation of meiosis resumption, possibility of concurrent activation and potential synergism between 17a,20b-dihydroxy-4-pregnen-3-one (DHP)-and Igf-mediated signalling cascades in alleviating E 2 inhibition of oocyte maturation (OM) has not been investigated. Here using zebrafish (Danio rerio) defolliculated oocytes, we examined the effect of DHP and Igf1, either alone or in combination, in presence or absence of E 2 , on OM in vitro. While priming of denuded oocytes with E 2 blocked spontaneous maturation, co-treatment with DHP (3 nM) and Igf1 (10 nM), but not alone, reversed E 2 inhibition and promoted a robust increase in germinal vesicle breakdown (GVBD). Although stimulation with either Igf1 or DHP promoted Akt phosphorylation, pharmacological inhibition of PI3K/Akt signalling prevented Igf1-induced GVBD but delayed DHP action till 4-5 h of incubation. Moreover, high intra-oocyte cAMP attenuates both DHP and Igf1-mediated OM and co-stimulation with DHP and Igf1 could effectively reverse E 2 action on PKA phosphorylation. Interestingly, data from in vivo studies reveal that heightened expression of igf1, igf3 transcripts in intact follicles corresponded well with elevated phosphorylation of Igf1r and Akt, mPRa immunoreactivity, PKA inhibition and accelerated GVBD response just prior to ovulation. This indicates potential synergism between maturational steroid and Igf1 which might have physiological relevance in overcoming E 2 inhibition of meiosis resumption in zebrafish oocytes.

show abstract

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Effect Of Camp/pka Regulation Of Dhp And/or Igf1 Induced Meimentioning

confidence: 99%

Section: Oocyte Preparation and In Vitro Culturementioning

confidence: 99%

Section: Oocyte Preparation and In Vitro Culturementioning

confidence: 99%

See 3 more Smart Citations

Releasing prophase arrest in zebrafish oocyte: synergism between maturational steroid and Igf1

Das¹,

Pal²,

Maitra³

2016

Reproduction

Self Cite

View full text Add to dashboard Cite

show abstract

Inhibition of Oocyte Maturation by Malathion and Structurally Related Chemicals in Zebrafish (Danio rerio) After In Vitro and In Vivo Exposure

Miller

Essen

Brinkmann

et al. 2022

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Oogenesis is the process by which a primary oocyte develops into a fertilizable oocyte, making it critical to successful reproduction in fish. In zebrafish (Danio rerio), there are five stages of oogenesis. During the final step (oocyte maturation), the maturation-inducing hormone 17α,20β-dihydroxy-4-pregnen-3-one (MIH) activates the membrane progestin receptor, inducing germinal vesicle breakdown. Using in vitro assays, it has been shown that anthropogenic stressors can dysregulate MIH-induced oocyte maturation. However, it is unknown whether the in vitro assay is predictive of reproductive performance after in vivo exposure. We demonstrate that a known inhibitor of oocyte maturation, malathion, and a structurally related chemical, dimethoate, inhibit oocyte maturation. However, malaoxon and omethoate, which are metabolites of malathion and dimethoate, did not inhibit oocyte maturation. Malathion and dimethoate inhibited maturation to a similar magnitude when oocytes were exposed for 4 h in vitro or 10 days in vivo, suggesting that the in vitro zebrafish oocyte maturation assay might be predictive of alterations to reproductive performance. However, when adult zebrafish were exposed to malathion for 21 days, there was no alteration in fecundity or fertility in comparison with control fish. Our study supports the oocyte maturation assay as being predictive of the success of in vitro oocyte maturation after in vivo exposure, but it remains unclear whether inhibition of MIH-induced oocyte maturation in vitro correlates to decreases in reproductive performance.

show abstract

Explorations of the optimal method for isolating oocytes from zebrafish (Danio rerio) ovary

et al. 2018

View full text Add to dashboard Cite

Obtaining oocytes from the adult female zebrafish (Danio rerio) ovary has enormous importance in the studies of developmental biology, toxicology, and genetics. It is vital to establish a simple and effective approach to ensure the quantity and quality of oocytes, which will enable the success of follow‐up experimental investigation finally. Usually, oocytes are separated with mechanical or enzymatic methods, however, little studies have been done with concerns about the comparative effects. The present study separated zebrafish oocytes of Stage III with five frequently used methods, including stripping, pipetting, hyaluronidase (1.6 mg/ml), collagenase (0.4 mg/ml), and trypsin (0.1%). The cell viability, oxidative stress, mitogen‐activated protein kinase (MAPK) protein phosphorylation, and apoptosis levels were selected as main biomarkers to evaluate the oocytes health status. The results showed that both trypsin and hyaluronidase isolation significantly upregulated germinal vesicle breakdown (GVBD) rates and downregulated p38 MAPK activity simultaneously. GVBD rates and survival rates were decreased notably in oocytes separated by the collagenase method. Above results indicate that zebrafish oocytes in vitro are sensitive to enzymatic treatments and the enzymatic isolation is not the suitable mean for collecting zebrafish oocytes although it is time‐saving. The mechanical strategy of pipetting remarkably increased the reactive oxygen species and malondialdehyde level in isolated oocytes. Interestingly, oocytes separated with stripping show less physiological and biochemical damages. Therefore, stripping isolation is comparatively recommended as the optimum method for separating and collecting numerous intact and healthy zebrafish oocytes in vitro for the subsequent developmental research.

show abstract

High cAMP attenuation of insulin-stimulated meiotic G2-M1 transition in zebrafish oocytes: Interaction between the cAMP-dependent protein kinase (PKA) and the MAPK3/1 pathways

Cited by 35 publications

References 74 publications

Releasing prophase arrest in zebrafish oocyte: synergism between maturational steroid and Igf1

Releasing prophase arrest in zebrafish oocyte: synergism between maturational steroid and Igf1

Inhibition of Oocyte Maturation by Malathion and Structurally Related Chemicals in Zebrafish (Danio rerio) After In Vitro and In Vivo Exposure

Explorations of the optimal method for isolating oocytes from zebrafish (Danio rerio) ovary

Contact Info

Product

Resources

About