High cell density fermentation of Gluconobacter oxydans DSM 2003 for glycolic acid production

Wei, Guodong; Yang, Xuepeng; Gan, Tula; Zhou, Wei; Lin, Jian; Wei, Dongzhi

doi:10.1007/s10295-009-0584-1

Cited by 52 publications

(30 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Importantly, in addition to relatively low price of glucose, our experiment results showed that similar final cell concentrations were achieved for both the GDHE50 strain on 10 g/l of glucose and the wild-type strain 621H on 73 g/l of sorbitol, while the latter high concentration of sorbitol was reported to be the optimal sorbitol concentration for G. oxydans DSM 2003 to gain high-density fermentation [5]. It might therefore suggest that the operating cost of biotransformation systems using the engineered G. oxydans in this study could be potentially reduced.…”

Section: Oxidation Of Ethylene Glycol To Glycolic Acidsupporting

confidence: 60%

See 1 more Smart Citation

Modification and Evolution of Gluconobacter oxydans for Enhanced Growth and Biotransformation Capabilities at Low Glucose Concentration

Zhu

Wei

et al. 2011

Mol Biotechnol

Self Cite

View full text Add to dashboard Cite

Gluconobacter oxydans is widely used in several biotechnological applications, where sorbitol or mannitol is commonly used as carbon source at high concentration. In this study, a membrane-bound glucose dehydrogenase-deficient strain (GDHK) was constructed to eliminate growth problems on glucose caused by direct oxidation of glucose in the medium. To achieve improved growth properties for the GDHK strain on glucose, a laboratory adaptive evolution experiment was performed with glucose as the sole carbon source. Results indicated evident, albeit modest, improvements in cell growth after a 50-day (about 430 generations) experimental evolution on glucose. The maximum specific growth rate and biomass yield of the resulting GDHE50 strain were increased around 1.35- to 1.4-fold compared with those of the GDHK strain. Meanwhile, two types of biotransformation reactions using resting cells of G. oxydans were investigated. Significant elevations in biotransformation performance of the GHDE50 strain were observed in comparison with that of the wild-type strain. In addition, resting cells of the GDHE50 strain grown on a relatively low concentration of glucose (10 g/l) could catalyze the biotransformation of glycerol to dihydroxyacetone and ethylene glycol to glycolic acid as efficient as the wild-type G. oxydans cultured on higher concentration of sorbitol or other carbon sources. These results suggest very favorable prospects of using glucose to lower production cost in many important industrial biocatalysis and biotransformation processes.

show abstract

Section: Oxidation Of Ethylene Glycol To Glycolic Acidsupporting

confidence: 60%

“…Several studies were carried out to optimize culture conditions of G. oxydsans on sorbitol or mannose which will not be directly converted into acids in the medium [5]. Due to relatively high cost in using above carbon sources, researchers have been trying to modify mGDH in G. oxydans.…”

Section: Introductionmentioning

confidence: 99%

Modification and Evolution of Gluconobacter oxydans for Enhanced Growth and Biotransformation Capabilities at Low Glucose Concentration

Zhu

Wei

et al. 2011

Mol Biotechnol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Gluconobacter oxydan is one of the most widely used industrial bacterium for the incomplete oxidization of a wide range of sugars, alcohols and polyols into their respective products . The complete genome sequence of G. oxydans was reported in 2005 .…”

Section: Introductionmentioning

confidence: 99%

Structural insights into substrate and coenzyme preference by SDR family protein Gox2253 from Gluconobater oxydans

et al. 2014

Self Cite

View full text Add to dashboard Cite

Gox2253 from Gluconobacter oxydans belongs to the short-chain dehydrogenases/reductases family, and catalyzes the reduction of heptanal, octanal, nonanal, and decanal with NADPH. To develop a robust working platform to engineer novel G. oxydans oxidoreductases with designed coenzyme preference, we adopted a structure based rational design strategy using computational predictions that considers the number of hydrogen bonds formed between enzyme and docked coenzyme. We report the crystal structure of Gox2253 at 2.6 Å resolution, ternary models of Gox2253 mutants in complex with NADH/short-chain aldehydes, and propose a structural mechanism of substrate selection. Molecular dynamics simulation shows that hydrogen bonds could form between 2'-hydroxyl group in the adenosine moiety of NADH and the side chain of Gox2253 mutant after arginine at position 42 is replaced with tyrosine or lysine. Consistent with the molecular dynamics prediction, Gox2253-R42Y/K mutants can use both NADH and NADPH as a coenzyme. Hence, the strategies here could provide a practical platform to engineer coenzyme selectivity for any given oxidoreductase and could serve as an additional consideration to engineer substrate-binding pockets.

show abstract

“…The partially oxidized products (aldehyde, ketone, and organic acid) are rapidly excreted into the medium. This property makes G. oxydans an important biocatalyst for industrial use [26-28]. In a previous study, many substrates including glycerol, meso-erythritol, 1,3-butanediol, and 2,3-butanediol could be oxidized by the membrane-bound polyol dehydrogenase (GOX 0854 and GOX 0855) in G. oxydans 621H [29].…”

Section: Resultsmentioning

confidence: 99%

Efficient bioconversion of 2,3-butanediol into acetoin using Gluconobacter oxydans DSM 2003

Wang

Zhang

et al. 2013

Biotechnol Biofuels

View full text Add to dashboard Cite

Background2,3-Butanediol is a platform and fuel biochemical that can be efficiently produced from biomass. However, a value-added process for this chemical has not yet been developed. To expand the utilization of 2,3-butanediol produced from biomass, an improved derivative process of 2,3-butanediol is desirable.ResultsIn this study, a Gluconobacter oxydans strain DSM 2003 was found to have the ability to transform 2,3-butanediol into acetoin, a high value feedstock that can be widely used in dairy and cosmetic products, and chemical synthesis. All three stereoisomers, meso-2,3-butanediol, (2R,3R)-2,3-butanediol, and (2S,3S)-2,3-butanediol, could be transformed into acetoin by the strain. After optimization of the bioconversion conditions, the optimum growth temperature for acetoin production by strain DSM 2003 was found to be 30°C and the medium pH was 6.0. With an initial 2,3-butanediol concentration of 40 g/L, acetoin at a high concentration of 89.2 g/L was obtained from 2,3-butanediol by fed-batch bioconversion with a high productivity (1.24 g/L · h) and high yield (0.912 mol/mol).ConclusionsG. oxydans DSM 2003 is the first strain that can be used in the direct production of acetoin from 2,3-butanediol. The product concentration and yield of the novel process are both new records for acetoin production. The results demonstrate that the method developed in this study could provide a promising process for efficient acetoin production and industrially produced 2,3-butanediol utilization.

show abstract

High cell density fermentation of Gluconobacter oxydans DSM 2003 for glycolic acid production

Cited by 52 publications

References 14 publications

Modification and Evolution of Gluconobacter oxydans for Enhanced Growth and Biotransformation Capabilities at Low Glucose Concentration

Modification and Evolution of Gluconobacter oxydans for Enhanced Growth and Biotransformation Capabilities at Low Glucose Concentration

Structural insights into substrate and coenzyme preference by SDR family protein Gox2253 from Gluconobater oxydans

Efficient bioconversion of 2,3-butanediol into acetoin using Gluconobacter oxydans DSM 2003

Contact Info

Product

Resources

About