High cell density fermentation via a metabolically engineered <i>Escherichia coli</i> for the enhanced production of succinic acid

Wang, Dan; Li, Qiang; Song, Ziyu; Zhou, Wei; Su, Zhiguo; Xing, Jianmin

doi:10.1002/jctb.2543

Cited by 40 publications

(19 citation statements)

References 33 publications

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“…coli , many possible improvements can be achieved to enhance α-pinene production. One approach is to optimize the fermentation process by increasing cell density to elevate the yield of product [29,30]. Another possibility is employing a chromosome integration technique to decrease the cell growth burden on the host that results from overexpression of heterologous genes [31,32].…”

Section: Resultsmentioning

confidence: 99%

Metabolic engineering of Escherichia colifor the biosynthesis of alpha-pinene

Yang

Nie

Ren

et al. 2013

Biotechnol Biofuels

142

122

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Backgroundα-Pinene is an important natural product that is widely used in flavorings, fragrances, medicines, fine chemicals and high-density renewable fuels. Currently, α-Pinene used in industry is mainly produced either by tapping trees (gum turpentine) or as a byproduct of paper pulping (crude sulfate turpentine, CST). However, the extraction of it from trees is tedious and inefficient and requires substantial expenditure of natural resources. Therefore, it is necessary to seek sustainable technologies for α-pinene production.ResultsTo construct the microbial synthetic pathway of α-pinene in E. coli, we co-expressed native geranyl diphosphate synthase (IspA) from E. coli and α-pinene synthase (Pt30) from Pinus taeda, and then to increase the geranyl diphosphate (GPP) content in the cells, a suitable geranyl diphosphate synthase (GPPS2) was selected from two different origins. Furthermore, to enhance α-pinene production, a novel biosynthetic pathway of α-pinene was assembled in E. coli BL21(DE3) with the heterologous hybrid mevalonate (MVA) pathway, GPPS2 and α-pinene synthase (Pt30). The final genetic strain, YJM28, harboring the above novel biosynthetic pathway of α-pinene, accumulated α-pinene up to 5.44 mg/L and 0.97 g/L under flask and fed-batch fermentation conditions, respectively. The conversion efficiency of glucose to α-pinene (gram to gram) in the metabolically engineered strain reached 2.61%.ConclusionsIn this paper, by using metabolic engineering techniques, the more efficient biosynthetic pathway of α-pinene was successfully assembled in E. coli BL21(DE3) with the heterologous hybrid MVA pathway, GPPS2 and α-pinene synthase (Pt30). In addition, this is the first report on α-pinene fed-batch fermentation, and our results represent improvements over previous reports.

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Section: Resultsmentioning

confidence: 99%

Metabolic engineering of Escherichia colifor the biosynthesis of alpha-pinene

Yang

Nie

Ren

et al. 2013

Biotechnol Biofuels

142

122

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“…One approach is to optimize the fermentation process by reducing the toxicity of substrate and product, such as in situ product removal, membrane technology and a two-stage fermentation using different substrates. 12,34,35 Genetic modication of the host is another possible approach: chromosome integration technique to decrease burden of cell metabolism, 36 construction of endproduct export systems, activation of general stress response genes, and expression of heat shock proteins to improve the tolerance of the host. [30][31][32]…”

Section: Inhibitory Effect Of Phloroglucinol To E Coli In the Acetatmentioning

confidence: 99%

Efficient conversion of acetate into phloroglucinol by recombinant Escherichia coli

Xian

Liu

2017

RSC Adv.

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“…Anaerobiospirillum succiniciproducens, Actinobacillus succinogenes, Mannheimia succiniciproducens, Basfia succiniciproducens and recombinant Escherichia coli are regarded as promising succinic acid producers, due to their high succinic acid productivity and ability to utilise a wide range of C5 and C6 sugars for fermentative succinic acid production (44). Moreover, utilisation of different agricultural residues and waste from diary industry as feedstock for fermentative succinic acid production (19,(45)(46)(47)(48) through bacteria have also been demonstrated (Table 3).…”

Section: Utilisation Of Hydrolysed Food Waste For High Value Chemicalmentioning

confidence: 99%