High cell density fermentations of Pseudomonas oleovorans for the production of mcl-PHAs in two-liquid phase media

Kellerhals, Michele B.; Hazenberg, Wil

doi:10.1016/s0141-0229(98)00113-6

Cited by 31 publications

(22 citation statements)

References 18 publications

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“…To achieve the efficient production of PHA, high PHA concentration and PHA productivity are necessary. There have been several reports on the production of MCL-PHA by Pseudomonas strains from octane or octanoic acid (Kellerhals et al, 1999;Preusting et al, 1993a,b). However, because of low oxygen solubility in octane, low PHA concentration was obtained with low PHA productivity (Weusthuis, 1996).…”

Section: Fed-batch Culturementioning

confidence: 99%

“…MCL-PHAs are biosynthesized by Pseudomonas oleovorans (Kellerhals et al, 1999;Lee, 1996a;Preusting et al, 1993a,b;Ramsay et al, 1991), Pseudomonas putida (Casini et al, 1997;Huijberts, 1996;Huijberts and Eggink, 1996;Kim et al, 1997), and other fluorescent Pseudomonas strains (Timm and Steinbüchel, 1990) from alkanes, alkenes, or alkanoic acids. However, to date, the final MCL-PHA concentration and PHA content obtained have been at relatively low levels compared with those of SCL-PHAs, which has hampered the development of MCL-PHA applications.…”

Section: Introductionmentioning

confidence: 99%

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Production of medium-chain-length polyhydroxyalkanoates by high-cell-density cultivation of Pseudomonas putida under phosphorus limitation

Lee¹,

Wong²,

Choi³

et al. 2000

Biotechnol. Bioeng.

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High-cell-density fed-batch cultures of Pseudomonas putida were carried out for the production of medium-chain-length polyhydroxyalkanoates (PHAs) using oleic acid as a carbon source. By employing an optimal feeding strategy without the limitation of any nutrient, a high cell concentration of 173 g/L was achieved, but the PHA concentration and PHA content were only 32.3 g/L and 18.7 wt%, respectively. To increase the PHA concentration and content, phosphorus limitation was applied during fed-bath culture by reducing the initial KH(2)PO(4) concentration. When the initial KH(2)PO(4) concentration was reduced to 4 g/L, cell concentration, PHA concentration, and PHA content obtained in 38 h were 141 g/L, 72. 6 g/L, and 51.4 wt%, respectively, resulting in a high productivity of 1.91 g PHA/L per hour.

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Section: Fed-batch Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Production of medium-chain-length polyhydroxyalkanoates by high-cell-density cultivation of Pseudomonas putida under phosphorus limitation

Lee¹,

Wong²,

Choi³

et al. 2000

Biotechnol. Bioeng.

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“…With a low temperature (18°C) during cultivation, a cell density of 35 g/liter CDM was obtained, and the cells contained 33% (wt/wt) PHA (49). On the other hand, when cells of P. putida Gpo1 were grown with a better oxygen transfer and in optimized medium, a cell density of 112 g CDM /liter was obtained; however, the cells contained only 25% PHA (32,34). One has also to consider that all previous cultivations were conducted at a small scale, not exceeding 30 liters (33).…”

Section: Discussionmentioning

confidence: 99%

“…Recovery procedures for PHO principally resemble those developed for PHB, which usually involve the application of halogenated solvents, such as chloroform (39,52). PHB recovery using hypochlorite (4,14,51), SDS in an in situ extraction process (64), or an enzyme cocktail (9,34) were also reported. The disadvantages related to applications of hypochlorite and detergents are the severe reduction in polymer molecular weight and the requirement of extended washing steps to get rid of detergent residuals, respectively.…”

Section: Discussionmentioning

confidence: 99%

Large-Scale Production of Poly(3-Hydroxyoctanoic Acid) by Pseudomonas putida GPo1 and a Simplified Downstream Process

Elbahloul

Steinbüchel

2009

Appl Environ Microbiol

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The suitability of Pseudomonas putida GPo1 for large-scale cultivation and production of poly(3-hydroxyoctanoate) (PHO) was investigated in this study. Three fed-batch cultivations of P. putida GPo1 at the 350-or 400-liter scale in a bioreactor with a capacity of 650 liters were done in mineral salts medium containing initially 20 mM sodium octanoate as the carbon source. The feeding solution included ammonium octanoate, which was fed at a relatively low concentration to promote PHO accumulation under nitrogen-limited conditions. During cultivation, the pH was regulated by addition of NaOH, NH 4 OH, or octanoic acid, which was used as an additional carbon source. Partial O 2 pressure (pO 2 ) was adjusted to 20 to 40% by controlling the airflow and stirrer speed. Under the optimized conditions, P. putida GPo1 was able to grow to cell densities as high as 18, 37, and 53 g cells (dry mass) (CDM) per liter containing 49, 55, and 60% (wt/wt) of PHO, respectively. The resulting 40 kg CDM from these three cultivations was used directly for extraction of PHO. Three different methods of extraction of PHO were applied. From these, only acetone extraction showed better performance and resulted in 94% recovery of the PHO contents of cells. A novel mixture of precipitation solvents composed of 70% (vol/vol) methanol and 70% (vol/vol) ethanol was identified in this study. The ratio of PHO concentrate to the mixture was 0.2:1 (vol/vol) and allowed complete precipitation of PHO as white flakes. However, at a ratio of 1:1 (vol/vol) of the solvent mixture to PHO concentrate, a highly purified PHO was obtained. Precipitation yielded a dough-like polymeric material which was cast into thin layers and then shredded into small strips to allow evaporation of the remaining solvents. Gas chromatographic analysis revealed a purity of about 99% ؎ 0.2% (wt/wt) of the polymer, which consisted mainly of 3-hydroxyoctanoic acid (96 mol%).

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“…Moreover, high cell density results in a low amount of caffeine available per cell reducing the net concentration of caffeine in the vicinity of the cells. It is also known that metabolism or accumulation of secondary metabolites occurs in the stationary phase of an organism [16]. Highest biomass production (2.64 g l −1 with and caffeine degradation rate of 0.053 g l −1 h −1 , was achieved at 48 h of caffeine dosing (96 h of growth).…”

Section: Biomass Productionmentioning

confidence: 99%

Effect of Physicochemical Parameters on Enzymatic Biodecaffeination During Tea Fermentation

Babu¹,

Thakur

Patra³

2011

Appl Biochem Biotechnol

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We report for the first time the development of a biodecaffeination process for tea synchronised with tea fermentation process using enzymes isolated from Pseudomonas alcaligenes. Cell-free extract was used for biodecaffeination of tea during fermentation of tea and 80% of the caffeine in the tea dhool was degraded within 90 min of incubation. Several factors that tend to effect the biodecaffeination during this stage, like moisture, aeration, intermittent enzyme addition and mixing, were optimized, and inhibitory interactions of proteins with polyphenols, caffeine-polyphenol interactions, which directly influence the biodecaffeination process were prevented by the use of glycine (5% w/w) in the dhool. Tea decaffeinated through the enzymatic route retained the original flavor and aroma, and there was an increase in the total polyphenol content of the tea.

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High cell density fermentations of Pseudomonas oleovorans for the production of mcl-PHAs in two-liquid phase media

Cited by 31 publications

References 18 publications

Production of medium-chain-length polyhydroxyalkanoates by high-cell-density cultivation of Pseudomonas putida under phosphorus limitation

Production of medium-chain-length polyhydroxyalkanoates by high-cell-density cultivation of Pseudomonas putida under phosphorus limitation

Large-Scale Production of Poly(3-Hydroxyoctanoic Acid) by Pseudomonas putida GPo1 and a Simplified Downstream Process

Effect of Physicochemical Parameters on Enzymatic Biodecaffeination During Tea Fermentation

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