High complexity of toxic reactions: parallels between products of oxidative stress and advanced glycation end products

Bolt, Hermann M.

doi:10.1007/s00204-020-02727-0

Cited by 5 publications

(2 citation statements)

References 17 publications

(15 reference statements)

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“…Glycation is the cascade of complex reactions where the sugar moiety non-covalently binds to free amino groups of protein and forms stable Amadori products. 55 MGO, a highly reactive dicarbonyl compound generated through glucose metabolism, altered the protein's structure and led to AGEs generation. Different analytical techniques, such as phenyl boronate affinity chromatography, are commonly used to estimate glycation adduct formation.…”

Section: Discussionmentioning

confidence: 99%

Homeopathic Formulations of Syzygium jambolanum Alleviate Glycation-Mediated Structural and Functional Modifications of Albumin: Evaluation through Multi-Spectroscopic and Microscopic Approaches

Bangar,

Ravindran,

Shaikh

et al. 2023

Homeopathy

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Background The growing interest in identifying the mode of action of traditional medicines has strengthened its research. Syzygium jambolanum (Syzyg) is commonly prescribed in homeopathy and is a rich source of phytochemicals. Objective The present study aims to shed light on the anti-glycation molecular mechanism of Syzyg mother tincture (MT), 30c, and 200c on glycated human serum albumin (HSA) by multi-spectroscopic and microscopic approaches. Methods The phytochemicals and antioxidant potential of the Syzyg formulations were estimated by the high-performance liquid chromatography and spectroscopic technique, respectively. Glycation was initiated by incubating HSA with methylglyoxal, three Syzyg formulations, and the known inhibitor aminoguanidine in separate tubes at 37°C for 48 hours. The formation of glycation adducts was assessed by spectrofluorometer and affinity chromatography. The structural modifications were analyzed through circular dichroism, Fourier transform infrared spectroscopy, turbidity, 8-anilinonapthalene-1-sulfonic acid fluorescence, and nuclear magnetic resonance. Further, the formation of the aggregates was examined by thioflavin T, native-polyacrylamide gel electrophoresis, and transmission electron microscopy. Additionally, the functional modifications of glycated HSA were determined by esterase-like activity and antioxidant capacity. The binding analysis of Syzyg formulations with glycated HSA was evaluated by surface plasmon resonance (SPR). Results Syzyg formulations MT, 30c, and 200c contained gallic acid and ellagic acid as major phytochemicals, with concentrations of 16.02, 0.86, and 0.52 µg/mL, and 227.35, 1.35, and 0.84 µg/mL, respectively. Additionally, all three formulations had remarkable radical scavenging ability and could significantly inhibit glycation compared with aminoguanidine. Further, Syzyg formulations inhibited albumin's structural and functional modifications. SPR data showed that Syzyg formulations bind to glycated HSA with an equilibrium dissociation constant of 1.10 nM. Conclusion Syzyg formulations inhibited the glycation process while maintaining the structural and functional integrity of HSA.

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Section: Discussionmentioning

confidence: 99%

Homeopathic Formulations of Syzygium jambolanum Alleviate Glycation-Mediated Structural and Functional Modifications of Albumin: Evaluation through Multi-Spectroscopic and Microscopic Approaches

Bangar,

Ravindran,

Shaikh

et al. 2023

Homeopathy

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“…The reaction between proteins and reducing sugars or carbonyl groups is known as the Maillard reaction, which non-enzymatically alters the function and morphology of these biological molecules (8). It mainly includes three levels (Figure 1).…”

Section: Formation Mechanism Of Agesmentioning

confidence: 99%

Advanced glycation end products in diabetic retinopathy and phytochemical therapy

Kang

Dai²,

Jiang³

et al. 2022

Front. Nutr.

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Advanced glycation end products (AGEs) are generated by the nonenzymatic glycation of proteins or lipids. Diabetic retinopathy (DR) is one common complication in patients with diabetes. The accumulation of AGEs in retinal cells is strongly associated with the development of DR. AGEs can induce the breakdown of redox balance and then cause oxidative stress in retinal cells, exerting cytopathic effects in the progression of DR. The interaction between AGEs and the receptor for AGE (RAGE) is involved in multiple cellular pathological alterations in the retina. This review is to elucidate the pathogenetic roles of AGEs in the progression of DR, including metabolic abnormalities, lipid peroxidation, structural and functional alterations, and neurodegeneration. In addition, disorders associated with AGEs can be used as potential therapeutic targets to explore effective and safe treatments for DR. In this review, we have also introduced antioxidant phytochemicals as potential therapeutic strategies for the treatment of DR.

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Trends in research on advanced glycation end products (AGEs)

Bolt,

Hengstler

2024

Arch Toxicol

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High complexity of toxic reactions: parallels between products of oxidative stress and advanced glycation end products

Cited by 5 publications

References 17 publications

Homeopathic Formulations of Syzygium jambolanum Alleviate Glycation-Mediated Structural and Functional Modifications of Albumin: Evaluation through Multi-Spectroscopic and Microscopic Approaches

Homeopathic Formulations of Syzygium jambolanum Alleviate Glycation-Mediated Structural and Functional Modifications of Albumin: Evaluation through Multi-Spectroscopic and Microscopic Approaches

Advanced glycation end products in diabetic retinopathy and phytochemical therapy

Trends in research on advanced glycation end products (AGEs)

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