High concentration DNA solubility in bio-ionic liquids with long-lasting chemical and structural stability at room temperature

Sharma, Mukesh; Mondal, Dibyendu; Singh, Nripat; Trivedi, Nitin; Bhatt, Jit Kumar; Prasad, Kamalesh

doi:10.1039/c5ra03512k

Cited by 35 publications

(31 citation statements)

References 32 publications

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“…For industrial purposes, ILs are being currently applied in DNA-based biosensors (Chen et al 2013;Sun et al 2013;Machado et al 2014), or to assist a vast variety of methods in genetic manipulations, including genomic sequencing (Kulkarni and Mukherjee 2016), cloning (Li et al 2013b), or gene delivery and transformation (Zhang et al 2009;Soni et al 2015). Moreover, replacement of standard aqueous solutions by ILs as solvents and catalysts for laboratory purposes enabled to overcome certain technological limitations associated with DNA nanotechnology (Nishimura et al 2005;Dandia et al 2013) due to enhanced DNA stability and solubility in ILs (Zhang et al 2009;Sharma et al 2015;Jumbri et al 2016;Mishra et al 2016). Among others, it has been shown that plasmid DNA demonstrates an increased resistance against enzymatic degradation in aqueous ILs based on choline dihydrogen phosphate when compared to saline buffer (Mazid et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Aqueous ionic liquids in comparison with standard co-solutes

Oprzeska-Zingrebe

Smiatek

2018

Biophys Rev

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Ionic liquids (ILs) are versatile solvents for a broad range of biotechnological applications. Recent experimental and simulation results highlight the potential benefits of dilute ILs in aqueous solution (aqueous ILs) in order to modify protein and DNA structures systematically. In contrast to a limited number of standard co-solutes like urea, ectoine, trimethylamine-N-oxide (TMAO), or guanidinium chloride, the large amount of possible cation and anion combinations in aqueous ILs can be used to develop tailor-made stabilizers or destabilizers for specific purposes. In this review article, we highlight common principles and differences between aqueous ILs and standard co-solutes with a specific focus on their underlying macromolecular stabilization or destabilization behavior. In combination with statistical thermodynamics theories, we present an efficient framework, which is used to classify structure modification effects consistently. The crucial importance of enthalpic and entropic contributions to the free energy change upon IL-assisted macromolecular unfolding in combination with a complex destabilization mechanism is described in detail. A special focus is also set on aqueous IL-DNA interactions, for which experimental and simulation outcomes are summarized and discussed in the context of previous findings.

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Section: Introductionmentioning

confidence: 99%

Aqueous ionic liquids in comparison with standard co-solutes

Oprzeska-Zingrebe

Smiatek

2018

Biophys Rev

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“…The electrostatic interactions between the solvent and 214 carrageenan in the hydrated DESs as well as their lower viscosity are perhaps responsible for 215 the observed higher yield of κ-carrageenan. Such interaction was found to be responsible for 216 selective agarose extraction from Gracilaria dura, an agarophyte(Sharma et al, 2015). The 217 indication of the interaction between the sulphate group of κ-carrageenan and the DESs are 218 also evident from the elemental analyses data (supporting…”

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confidence: 63%

“…78 The unique characteristics of ILs made them useful in many processes in carbohydrate 79 chemistry (Murugesan & Linhardt, 2005). ILs having the imidazolium structure, such as 1- we have reported selective precipitation of agarose from seaweed (Gracilaria dura) 88 extractives using bio-based ionic liquids (Sharma et al, 2015). It should be noted that, κ-89 carrageenan is conventionally extracted using alkali following the method reported by 90 Craigie & Leigh (1978).…”

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confidence: 98%

Deep eutectic solvents as efficient solvent system for the extraction of κ-carrageenan from Kappaphycus alvarezii

Das

Sharma

Mondal

et al. 2016

Carbohydrate Polymers

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145

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“…However, the stability of DNA in the DES depends on the ability of DNA to maintain its helical structure, that could change during the interaction of DNA with other molecules. Recently, Sharma et al found that hydrogen bonding and electrostatic interactions between bio-ionic liquids and DNA were found to be responsible for the long-term stability of DNA [5]. This finding enhanced the importance of studying the mode of interaction between DNA-DES to provide a stable medium for DNA.…”

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confidence: 91%

Studies of Interaction Between Tetrabutylammonium Bromide Based Deep Eutectic Solvent and Dna Using Fluorescence Quenching Method and Circular Dichroism Spectroscopy

Rahman¹

2016

MJAS

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The interaction of tetrabutylammonium bromide (TBABr)-based deep eutectic solvents (DESs) with calf thymus DNA (ct-DNA) was investigated by fluorescence and circular dichroism (CD) spectroscopy. The influences of hydrogen bond donors (HBDs), with respect to chain lengths and the presence of OH group were determined through ethidium bromide (EB) fluorescence quenching. Based on the quenching process, it was concluded that hydrophobic interactions were responsible for the DES ability to quench EB from EB-bound DNA. The strong electrostatic attraction between TBA + cation on DES and negative charge phosphate DNA was proved from fluorescence and CD spectroscopy. DNA was able to maintain its double helical structure at 25 °C with a concentration of 25% DES.Keywords: binding, deep eutectic solvent, tetrabutylammonium bromide, fluorescence quenching, circular dichroism spectroscopy Abstrak Ikatan antara pelarut eutektik mendalam berasaskan tetrabutilammonium bromida (TBABr) dengan 'calf thymus' DNA (ct-DNA) telah diuji dengan spektroskopi pendarfluor dan edaran dikroisme (CD). Pengaruh penderma ikatan hidrogen (HBDs) berdasarkan panjang rantai dan kehadiran kumpulan OH telah ditentukan melalui pelindapkejutan pendarfluor etidium bromida (EB). Berdasarkan proses pelindapkejutan, dapat dirumuskan bahawa ikatan hidrofobik berperanan dalam menentukan keupayaan DES untuk menyingkirkan EB daripada EB-terikat DNA. Tarikan elektrostatik yang kuat antara kation TBA + pada DES dengan cas negatif fosfat DNA telah dibuktikan daripada kedua-dua spektroskopi pendarfluor dan CD. DNA berupaya mengekalkan struktur heliks berganda pada suhu 25 °C dan kepekatan DES pada 25%.Katakunci: ikatan, pelarut eutektik mendalam, tetrabutilammonium bromida, pelindapkejutan pendarfluor, spektroskopi edaran dikroisme ISSN -2506 Rizana et al: STUDIES OF INTERACTION BETWEEN TETRABUTYLAMMONIUM BROMIDE BASED DEEP EUTECTIC SOLVENT AND DNA USING FLUORESCENCE QUENCHING METHOD AND CIRCULAR DICHROISM SPECTROSCOPY 1234Introduction DNA is a biomacromolecule that plays an important role in the living organism because it stores the genetic information for cell replication and transcription. The potentials of DNA were increasingly recognized especially in the applications of biosensors, catalytic membranes, absorbing membranes and support for catalysts [1,2]. However, DNA is unstable in aqueous solution, resulting in denaturation of nucleic acid structure after 1 month of storage at ambient temperature [3]. Over the past few years, new solvents that can retain the DNA helical structure has been researched. Mamajanov et al. were among the first to propose deep eutectic solvent (DES) as an alternative solvent to maintain DNA helical structure and it was found that choline chloride: urea can maintain the DNA native structure [4]. However, the stability of DNA in the DES depends on the ability of DNA to maintain its helical structure, that could change during the interaction of DNA with other molecules. Recently, Sharma et al. found that hydrogen bonding and e...

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High concentration DNA solubility in bio-ionic liquids with long-lasting chemical and structural stability at room temperature

Abstract: DNA (Salmon testes) was solubilized in a biocompatible ionic liquid at up to 8 wt% with long term (one year) structural and chemical stability upon storage at room temperature.

Cited by 35 publications

References 32 publications

Aqueous ionic liquids in comparison with standard co-solutes

Aqueous ionic liquids in comparison with standard co-solutes

Deep eutectic solvents as efficient solvent system for the extraction of κ-carrageenan from Kappaphycus alvarezii

Studies of Interaction Between Tetrabutylammonium Bromide Based Deep Eutectic Solvent and Dna Using Fluorescence Quenching Method and Circular Dichroism Spectroscopy

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