High content screening: seeing is believing

Zanella, Fabian; Lorens, James B.; Link, Wolfgang

doi:10.1016/j.tibtech.2010.02.005

Cited by 355 publications

(279 citation statements)

References 76 publications

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“…In this retrospective study, we examined ICSI outcomes of 1924 infertile patients who had SDF levels measured by the sperm chromatin integrity test (SCIT), an assay based upon SCSA principles but utilizing an imaging-based high-content screening platform (Zanella et al, 2010). Furthermore, we examined whether or not common laboratory interventions used to treat patients with high levels of SDF improved clinical outcomes.…”

Section: Introductionmentioning

confidence: 99%

Intervention improves assisted conception intracytoplasmic sperm injection outcomes for patients with high levels of sperm DNA fragmentation: a retrospective analysis

Bradley¹,

McArthur²,

Gee³

et al. 2016

Andrology

105

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SUMMARYSperm DNA fragmentation (SDF) is used in assisted reproductive technology (ART) programs as an indicator for sperm quality, although there is still a lack of consensus as to its clinical utility. In this retrospective study, we examined intracytoplasmic sperm injection (ICSI) outcomes of 1924 infertile patients who underwent SDF analysis using the sperm chromatin integrity test. ART patients were classified as having low [DNA fragmentation index (DFI) <29%] or high SDF (DFI ≥29%) and by whether or not an intervention [physiological intracytoplasmic sperm injection (PICSI), intracytoplasmic morphologically selected sperm injection (IMSI), testicular sperm extraction (TESE)/testicular sperm aspiration (TESA), frequent ejaculation] was performed. High SDF patients who did not have an intervention had a lower fertilization rate and poorer clinical outcomes from blastocyst transfers as compared with low SDF patients; the fertilization rate was 66.0% vs. 70.2% (p = 0.042), single embryo transfer (SET) fetal heart pregnancy rate was 28.5% vs. 45.2% (p = 0.042), and SET live birth rate was 24.9% vs. 40.6% (p = 0.060), respectively. Furthermore, high SDF patients who had an intervention had significantly improved blastocyst transfer outcomes, similar to those of low SDF patients; the SET live birth rate for high SDF intervention patients was 43.8% as compared with 24.9% for high SDF no intervention patients (p = 0.037) and 40.6% for low SDF patients (p = 0.446). Analysis of the three main intervention subgroups for high SDF patients revealed that TESE/TESA patients had the highest SET live birth rate; in comparison with 24.2% for high SDF patients who did not have an intervention, PICSI patients had 38.3% (p = 0.151), IMSI patients had 28.7% (p = 0.680), and TESE/TESA patients had 49.8% (p = 0.020). Our data suggest that SDF results indicate ICSI outcomes and that patients who have high SDF benefit from an intervention.

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Section: Introductionmentioning

confidence: 99%

Intervention improves assisted conception intracytoplasmic sperm injection outcomes for patients with high levels of sperm DNA fragmentation: a retrospective analysis

Bradley¹,

McArthur²,

Gee³

et al. 2016

Andrology

105

View full text Add to dashboard Cite

show abstract

“…It has also been recommended that, in general, hit compounds identified from a primary screen be further confirmed using a series of more functional secondary assays and analyzed using informatics tools [29,45,46] . Before a compound enters mechanistic studies, it is normally optimized through structure-activity relationship studies to improve its properties, such as increasing its potency and specificity and enhancing its pharmacokinetic properties.…”

Section: A Series Of Secondary Assaysmentioning

confidence: 99%

Embryonic stem cell application in drug discovery

Lou¹,

Liang²

2011

Acta Pharmacol Sin

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Embryonic stem (ES) cells and their differentiated progeny offer tremendous potential for regenerative medicine, even in the field of drug discovery. There is an urgent need for clinically relevant assays that make use of ES cells because of their rich biological utility. Attention has been focused on small molecules that allow the precise manipulation of cells in vitro, which could allow researchers to obtain homogeneous cell types for cell-based therapies and discover drugs for stimulating the regeneration of endogenous cells. Such therapeutics can act on target cells or their niches in vivo to promote cell survival, proliferation, differentiation, and homing. In the present paper, we reviewed the use of ES cell models for high-throughput/content drug screening and toxicity assessment. In addition, we examined the role of stem cells in large pharmaceutical companies' R&D and discussed a novel subject, nicheology, in stem cellrelated research fields.

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“…Development of precise robotics has resulted in the creation of automated image-based high-content screening (IBHCS) systems. These systems are capable of imaging multiple stains of large numbers of cell populations in a short period of time [1]. Given that the samples are typically placed in microwell plates, containing up to 1536 wells per plate, this represents a perfect setting for high-throughput approaches.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Flow Cytometry and Image-Based Screening for Cell Cycle Analysis

Matuszewski

Sintorn

Carreras-Puigvert

et al. 2016

Lecture Notes in Computer Science

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Abstract. Quantitative cell state measurements can provide a wealth of information about mechanism of action of chemical compounds and gene functionality. Here we present a comparison of cell cycle disruption measurements from commonly used flow cytometry (generating onedimensional signal data) and bioimaging (producing two-dimensional image data). Our results show high correlation between the two approaches indicating that image-based screening can be used as an alternative to flow cytometry. Furthermore, we discuss the benefits of image informatics over conventional single-signal flow cytometry.

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High content screening: seeing is believing

Cited by 355 publications

References 76 publications

Intervention improves assisted conception intracytoplasmic sperm injection outcomes for patients with high levels of sperm DNA fragmentation: a retrospective analysis

Intervention improves assisted conception intracytoplasmic sperm injection outcomes for patients with high levels of sperm DNA fragmentation: a retrospective analysis

Embryonic stem cell application in drug discovery

Comparison of Flow Cytometry and Image-Based Screening for Cell Cycle Analysis

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