High-Contrast Fluorescence Imaging Based on the Polarization Dependence of the Fluorescence Enhancement Using an Optical Interference Mirror Slide

Abstract

High-contrast fluorescence imaging using an optical interference mirror (OIM) slide that enhances the fluorescence from a fluorophore located on top of the OIM surface is reported. To enhance the fluorescence and reduce the background light of the OIM, transverse-electric-polarized excitation light was used as incident light, and the transverse-magnetic-polarized fluorescence signal was detected. As a result, an approximate 100-fold improvement in the signal-to-noise ratio was achieved through a 13-fold enhancement of the fluorescence signal and an 8-fold reduction of the background light.

Determination of Trace Rhodamine B in Chili Oil by Deep Eutectic Solvent Extraction and an Ultra High-Performance Liquid Chromatograph Equipped with a Fluorescence Detector

“…1, was used as an artificial synthetic xanthene dye, which was initially widely used in textiles and plastic industries as a colorant, and in biological chemistry as a fluorescent reagent. [1][2][3] Rhodamine B was reported to have carcinogenic properties, neurotoxicity toxicity, and chronic toxicity to humans and animals. 4,5 As a result of its harmful effects on human health, rhodamine B was identified as an illegal additive in food by the European Food Safety Authority.…”

Enhancement of Fluorescence-Based Sandwich Immunoassay Using Multilayered Microplates Modified with Plasma-Polymerized Films

“…The multilayered substrate fabricated with approximately 100-nm-thick dielectric LiF as an optical interference layer on the surface of a silver mirror can exhibit up to 400-fold enhancement of fluorescence for Rhodamine B compared with a bare glass slide [ 9 , 10 ]. Other dielectric materials instead of LiF, such as SiO 2 [ 11 ] and Al 2 O 3 [ 12 , 13 ], have also been utilized for the optical interference layers. Those modified substrates have been applied in many bioassays [ 14 , 15 , 16 ].…”