High copper concentrations produce genotoxicity and cytotoxicity in bovine cumulus cells

Anchordoquy, Juan Mateo; Nikoloff, Noelia; Pascua, Ana Malen; Furnus, Cecilia Cristina

doi:10.1007/s11356-017-9683-0

Cited by 12 publications

(3 citation statements)

References 90 publications

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“…Though these studies highlight the beneficial role of Cu on oocyte development, it is important to recognize that Cu can have cytotoxic and genotoxic behaviors. Cumulus cells exposed to high Cu concentrations (≥120 ug/dL) had lower mitochondrial activity, heightened incidence of apoptosis, and increased DNA damage compared to unexposed cells [27]. Cumulus cells are critical to oocyte viability, as they provide nutritive support and protect against damage during maturation [28,29].…”

Section: Reproductionmentioning

confidence: 99%

Impacts of Bovine Trace Mineral Supplementation on Maternal and Offspring Production and Health

Emon

Sanford

McCoski

2020

Animals

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Nutritional status can have major implications for animal health and production. Energy balance is easily determined using a body condition scoring system. This allows producers to readily adjust diets to meet an animal’s needs. Far less obvious is an animal’s trace mineral status, which is typically not assessed until an animal’s performance falls below expectation or illness is detected. Trace mineral toxicities and deficiencies can manifest as reduced thriftiness and/or poor reproductive performance, resulting in economic consequences for producers. Maternal mineral status not only impacts dam heath, but also the health of subsequent offspring. Both the oocyte and embryo are susceptible to changes in maternal mineral status. This susceptibility is maintained throughout fetal development via placental control of nutrient transfer to the fetal system. Furthermore, maternal mineral status continues to impact offspring health via colostrum and milk quality. Herein we discuss the roles of trace minerals in bovine reproductive performance, maternal health, colostrum and milk quality, and offspring health.

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Section: Reproductionmentioning

confidence: 99%

Impacts of Bovine Trace Mineral Supplementation on Maternal and Offspring Production and Health

Emon

Sanford

McCoski

2020

Animals

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“…As a result, it is possible to improve the resistance of sheep to Cu deficiency and excess by appropriate cross breeding and selection programs (Stevanovic et al, 2015). In recent years, copper toxicity of sheep has been increasing with the intensification of sheep industry, increased housing of ewes and use of breeds susceptible to Cu poisoning, such as the Texel and the Merino (Anchordoquy et al, 2017). The results of data analysis showed a large variation (Cv = 64.03%) in the concentration of Cu in blood samples (n = 780).…”

Section: Resultsmentioning

confidence: 99%

The Impact of Breed, Testing Time and Metabolic Profile on the Variation of Copper Concentration in Sheep Blood Serum

Autukaitė

Juozaitienė

Antanaitis

et al. 2021

IJAR

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Background: Copper supplementation in ruminants deserves special attention because of the narrow margin between deficiency and toxicity. The aim of the study was to analyse the relationship between the Cu concentration and other blood parameter values having taken into consideration their physiological limits based on the variation of copper concentration in the three different sheep breeds. Methods: Sixty sheep (35±5 kg) kept on the same farm were studied: the Suffolk (n = 20), the Merino (n = 20) and the Lithuanian blackhead (n = 20). The sheep were selected according to the following parameters: 3.5±0.3 years old, not pregnant during the entire investigation period, clinically healthy. All sheep were kept under the same conditions and throughout the year, were fed at the same time the same ration balanced according to their physiological needs. Individual blood samples were collected on the same day, on monthly basis for the period of one year (April 2018 to May 2019). Blood biochemical parameters as well blood haematology and cortisol concentration were analysed.Result: The highest average value of copper concentration was found in the Merino breed. The study has shown that the lowest number of blood samples with the levels of copper meeting the physiological limit values was found in the native Lithuanian blackhead sheep at the end of the grazing period and in the Merino and the Suffolk breeds - during the coldest winter months. The increase in copper concentration could be related to the stress caused due to the changes in dietary conditions, the variations of ambient temperature and different breed response to these factors.

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“…The collection of PLs is very easy, and it is encouraged to use them as an alternative for target tissues with the intention of investigating the effect of environmental genotoxic agent on animal/human or to explore the in vitro metabolism of that agent (Pavanello and Levis 1994). Previously, we demonstrated that CC culture was useful for evaluating cytotoxicity and genotoxicity (Anchordoquy et al 2017). In the present study, we established that EFZ induced genotoxic effects in PL and cytotoxic effects in both PL and CC cultures.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic and genotoxic effects induced by enrofloxacin-based antibiotic formulation Floxagen® in two experimental models of bovine cells in vitro: peripheral lymphocytes and cumulus cells

Anchordoquy

Nikoloff

Gambaro

et al. 2018

Environ Sci Pollut Res

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The in vitro effect of enrofloxacin (EFZ) was tested on two experimental somatic bovine cells in vitro: peripheral lymphocytes (PLs) and cumulus cells (CCs). The cytotoxicity and genotoxicity of this veterinary antibiotic were assessed using 3-(4,5dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assays, single-cell gel electrophoresis (SCGE) assay, and cytokinesis-block micronucleus cytome (CBMN cyt) assay. Cells were treated during 24 h, and three concentrations were tested (50 μg/mL, 100 μg/mL, 150 μg/mL). When EFZ was tested in PLs, the results demonstrated that the antibiotic was able to induce cell death and DNA damage with all concentrations. In addition, 50 μg/mL and 100 μg/mL EFZ increased frequencies of micronuclei (MNi). On the other hand, the highest EFZ concentration occasioned cellular cytotoxicity in CCs as evidenced by mitochondrial activity alterations. Nevertheless, EFZ was not able to induce DNA damage and MNi in CCs. These results represent the first experimental evidence of genotoxic and cytotoxic effects exerted by EFZ in bovine PLs and CCs.

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High copper concentrations produce genotoxicity and cytotoxicity in bovine cumulus cells

Cited by 12 publications

References 90 publications

Impacts of Bovine Trace Mineral Supplementation on Maternal and Offspring Production and Health

Impacts of Bovine Trace Mineral Supplementation on Maternal and Offspring Production and Health

The Impact of Breed, Testing Time and Metabolic Profile on the Variation of Copper Concentration in Sheep Blood Serum

Cytotoxic and genotoxic effects induced by enrofloxacin-based antibiotic formulation Floxagen® in two experimental models of bovine cells in vitro: peripheral lymphocytes and cumulus cells

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