High current, low redox potential mediatorless bioanode based on gold nanoparticles and glucose dehydrogenase from Ewingella americana

Nanocatalysts Containing Direct Electron Transfer-Capable Oxidoreductases: Recent Advances and Applications

“…It is known that the efficiency of the DET and an overpotential of the enzyme catalyzed reaction depends on the surface charge of the nanomaterial [134]. For example, our group demonstrated that the modification of AuNPs using positive charge inducing molecules (e.g., 4-aminothiophenol, cysteamine) results in a significantly improved DET for GDH and overpotential decrease in 0.3 V [97]. However, when several enzymes are used to design the nanocatalysts, it may be difficult to use specific surface modifications-one surface modification may increase the efficiency of DET for one enzyme, but decrease it for another.…”

“…This resulted in the biocathode exhibiting good performance-the system was able to operate in electrolyte solutions at a broad pH range and in the presence of high fluoride concentrations. Bioanodes with DET-capable oxidoreductases were also developed, for example, cellobiose dehydrogenase [93,94], fructose dehydrogenase [95], glucose dehydrogenase [96][97][98], and alcohol dehydrogenases [99,100]. Our group has also been working in this field-recently, we have demonstrated a DET bioanode system with glucose dehydrogenase from Ewingella americana, immobilized using gold nanoparticle and polyaniline nanocomposite, which exhibited exceptional performance towards glucose oxidation and operated in whole human blood [101].…”

“…However, to this day, no one successfully demonstrated enzymatic (nano) catalysts capable of producing hydrogen from carbohydrates and HER using carbohydrates has been demonstrated using H 2 producing bacteria cultures only [132]. If the reaction is thermodynamically possible (i.e., E ≥ 0 at reaction conditions), the potential incompatibility could be solved using various methods, which improves the DET contact between oxidoreductase and nanomaterial, as a fast DET usually results in low overpotential [97,100]. The first approach could be a rational engineering of the enzyme to produce novel enzyme homologues, which could operate in DET reactions faster or with lower overpotentials.…”

Development of Reasonably Stable Chitosan Based Proton Exchange Membranes for a Glucose Oxidase Based Enzymatic Biofuel Cell

“…High performance and longterm stability are necessary for commercial applications [31,[33][34][35]. Therefore, many studies were performed for enzyme electrode development to increase performance [35][36][37][38][39][40][41][42][43]. One the other hand, one of the important challenges for the enzymatic biofuel cell applications is membrane development for proton transfer since popular fuel cell membranes (i. e. perfluorosulfonic acid type membranes like Nafion ® ) are found as not effective because of cation inhibition [6,[18][19].…”

Dioxygen insensitive C70/AuNPs hybrid system for rapid and quantitative glucose biosensing

“…11 Particularly, gold nanoparticles (AuNPs), due to their size dependent electronic properties, 12 high active surface area, 13 biocompatibility 14,15 and stability of the composite material made thereof became a promising platform for biosensors comprising enzymes, the more as the hybrid formulations preserved the original enzymatic activity. 16,17 Moreover, the advantageous features of AuNPs can be enhanced by capping the gold surface with electroactive ligands. Numerous reports show that fullerene assemblies serve as excellent electron acceptors, 18 enhance charge separation and transport, 19,20 thus substantiating applications of C 60 fullerene and its derivatives in glucose biosensing.…”