Context. Nutritional standards usually assume that ~10% of the total nitrogen (TN) in forages is indigestible and hence not available to the ruminant. Senesced tropical C 4 grasses often contain TN concentrations that are marginal or deficient to meet the nutrient requirements of ruminants, and low TN availability will exacerbate N deficiencies. Aim. The aim of the study was to estimate the availability (i.e. digestibility) to ruminants of TN in mature and senesced C 4 grasses, using data derived from previous experiments and published data. Methods. In Dataset 1, forages grown in subtropical or tropical environments, including C 4 (n = 143) and C 3 (n = 15) grasses, were analysed for parameters including TN, acid detergent fibre (ADF), and N insoluble in ADF solution (ADIN). ADIN was used as a measure of unavailable TN. The Dataset 2 analysis included published measurements of TN and ADIN in C 4 (n = 187) and C 3 (n = 45) grasses. Key results. In Dataset 1, TN averaged 9.7 and ADIN 1.45 g N/kg diet DM in C 4 grasses. ADIN concentration could be predicted from TN and ADF concentrations by multiple regression (R 2 = 0.50; P < 0.001). The ratio ADIN/TN averaged 170 g/kg and increased exponentially with increasing ADF concentration (R 2 = 0.43; P < 0.001). Also, ADIN/TN was inversely related to TN concentration and DM digestibility. In C 4 grasses containing >400 and >500 g ADF/kg DM, ADIN/TN averaged 190 and 230 g/kg, respectively, and in those containing <10 g TN/kg DM, ADIN/TN averaged 194 g/kg. In these low-quality C 4 grasses only ~80%, and as little as ~50%, of TN was available to ruminants. Dataset 2 produced similar results, although C 4 grass forages were generally of higher quality, most having been harvested during vegetative growth. ADIN/ TN was much lower in C 3 grasses (89 g/kg). Conclusions. In senesced, low-quality C 4 grasses containing >400 g ADF, <10 g TN or <530 g digestible DM/kg, the proportion of TN available to ruminants is substantially lower than that assumed for forages in general. Implications. Low availability of TN in many senesced C 4 grasses needs to be considered when evaluating the adequacy of dietary TN for ruminants grazing senesced tropical grass pastures.