High-Definition Video System for Peripheral Neurorrhaphy in Rats

Abstract

It is possible to perform a peripheral neurorrhaphy in rats through video system magnification, but with a longer surgical time.

Does the type of electrode affect the electromyoneurographic parameters in rats?

“…The present study has some limitation: It was not evaluated the sensory and mixed nerve conduction 8,15 and in surgical post-operation (end-to-end 9 , 15 , end-to-side 8 , 11 , 16 and side-to-side 17 neurorrhaphy). If the study had been conducted in this scenario, the electrode’s type could affect the results.…”

“…Surface electrodes have the advantage of being a non-invasive method, and can be used with only the sedation of the animals, facilitating the accomplishment of multiple evaluations mainly in follow-up studies 5 , 11 . However, there is a need to know the local anatomy well to avoid overlapping with other muscle groups or to obtain a partial reading of the target muscle group 9 .…”

“…The gastrocnemius and cranial tibial muscles were chosen because of their known innervation by the tibial and common fibular nerves, respectively branches of the sciatic nerve, which is the most frequently used as the model system of peripheral nerve injury 8 , 9 , 11 , 12 .…”

“…The temperature was set on 24ºC. The electrophysiologic testing protocol was the following: 1) The rats were anesthetized with intraperitoneal ketamine (70 mg/kg) and xylazine (10 mg/kg) 8 , 9 and then shaved and placed in a horizontal supine position. 2) The temperature was measured with infrared thermometer at the time of examination.…”

“…4) All animals were submitted to two electroneuromyography analysis: first with surface electrode and then by needle electrode; by a Neuropack S1 MEB- 9400 © (Nihon Kohden, Japan). 5) For each compound muscle action potential, the latency, amplitude, duration and area of the negative peak of the gastrocnemius and cranial tibial muscles were measured and recorded 5 , 8 , 9 .…”