High density cultivation of BSK cells on sintered alumina ceramic foam support

Lee, D. W.; Grace, John R.; Chow, Billy K. C.; MacGillivray, Ross T. A.; Kilburn, Douglas G.

doi:10.1007/bf00556293

Cytotechnology

1991

DOI: 10.1007/bf00556293

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High density cultivation of BSK cells on sintered alumina ceramic foam support

D. W. Lee¹,

John R. Grace²,

Billy K. C. Chow³

et al.

Abstract: A perfusion system which utilizes a porous ceramic core has been tested for the cultivation of transformed BHK cells which produce human transferrin. A design is presented in which cells are immobilized within the porous ceramic particle and are fed by continuous perfusion of batch liquid medium. It was found that more than 5 x 10(9) BHK cells could be supported within the 40 mL ceramic matrix, a ten-fold increase in cell density per unit surface area over the standard roller bottle cultures or a five-fold inc… Show more

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Cited by 2 publications

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Long‐Term continuous culture of hepatocytes in a packed‐bed reactor utilizing porous resin

Miyoshi

Yanagi

Ohshima

et al. 1994

Biotech & Bioengineering

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As part of our attempt to develop a hybrid artificial liver support system using cultured hepatocytes, we investigated the long-term metabolic function of hepatocytes incubated in a packed-bed type reactor using reticulated polyvinyl formal (PVF) resin as a supporting material. Long-term (up to 1 week) perfusion culture experiments using the packed-bed reactor (20 mm i.d.) loaded with 500 PVF resin cubes (mean pore size 250 mum, 2 x 2 x 2 mm), together with conventional monolayer culture experiments as controls, were performed in serum-free or serum-containing medium. Ammonium metabolism and urea synthesis activities were evaluated quantitatively based on reaction kinetic analyses. Initial rates of ammonium metabolism and urea-N synthesis, as well as GPT enzyme activities, were adopted as indexes of the metabolic performance of the reactor and activities of the cultured hepatocytes.When serum-free medium was used in the perfusion cultures, ammonium metabolic and urea-N synthetic rates showed significant decay with elapse of the culture period, being less than 10% of those measured on day 1. This loss of activity was more prominent in the perfusion culture than in the monolayer cultures using this medium. In contrast, when serum-containing medium was used, approximately 50% of these activities obtained on day 1 were maintained even at the end of the cultures both in the perfusion and monolayer culture experiments.We concluded that the packed-bed reactor using PVF resin enabled high-density culture of hepatocytes, and showed a satisfactory ability to maintain the metabolic function of immobilized hepatocytes for relatively long periods of up to 1 week. This type of reactor is thus considered to represent a breakthrough in overcoming the difficulties involved in the development of a hybridtype artificial liver support system. (c) 1994 John Wiley & Sons, Inc.

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Long‐Term continuous culture of hepatocytes in a packed‐bed reactor utilizing porous resin

Miyoshi

Yanagi

Ohshima

et al. 1994

Biotech & Bioengineering

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A multiparallel bioreactor for the cultivation of mammalian cells in a 3D‐ceramic matrix

Goralczyk

Driemel

Bischof

et al. 2009

Biotechnology Progress

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For adherently growing cells, cultivation is limited by the provided growth surface. Excellent surface-to-volume ratios are found in highly porous matrices, which have to face the challenge of nutrient supply inside the matrices' caverns. Therefore, perfusion strategies are recommended which often have to deal with the need of developing an encompassing bioreactor periphery. We present a modular bioreactor system based on a porous ceramic matrix that enables the supply of cells with oxygen and nutrients by perfusion. The present version of the reactor system focuses on simple testing of various inoculation and operation modes. Moreover, it can be used to efficiently test different foam structures. Protocols are given to set-up the system together with handling procedures for long-time cultivation of a CHO cell line. Experimental results confirm vital growth of cells inside the matrices' caverns.

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High density cultivation of BSK cells on sintered alumina ceramic foam support

Cited by 2 publications

References 10 publications

Long‐Term continuous culture of hepatocytes in a packed‐bed reactor utilizing porous resin

Long‐Term continuous culture of hepatocytes in a packed‐bed reactor utilizing porous resin

A multiparallel bioreactor for the cultivation of mammalian cells in a 3D‐ceramic matrix

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