High density cultivation of plant cells in a new aeration-agitation type fermentor, maxblend fermentor®

Design of Mixing Systems for Plant Cell Suspensions in Stirred Reactors

“…The fresh weight:dry weight ratio of 15:1 is typical of actual values (Curtis and Emery, 1993; Snape et al, 1989); the cell concentration in fresh weight terms is therefore 450 kg m − 3 . The nominated cell aggregate size of 500 μm is a representative average of many values reported in the literature (Curtis and Emery, 1993; Kato et al, 1978; Leckie et al, 1991a; Scragg et al, 1988b; Tanaka, 1982; Yokoi et al, 1993), as is the specific gravity of 1.03 for plant cells cultivated in vitro (Ananta et al, 1995; Ballica and Ryu, 1993; Tanaka, 1982).…”

Bioreactor engineering for recombinant protein production in plant cell suspension cultures

“…To meet a general oxygen uptake rate of 5-10 mmol-O 2 /(L-h) for plant cell cultures, a typical volumetric oxygen mass transfer coefficient (k L a) value required in a bioreactor operation for plant cell cultures is between 10 and 50 h −1 [111], which is lower than that for microbial fermentation (100-1000 h −1 ) [112,113] and slightly higher than that of mammalian cell culture (0.25-10 h −1 ). In addition, aeration rates in the range of 0.05-0.1 vvm (volume of gas per volume of culture per minute) are typically used in a stirred-tank bioreactor and 0.5-1 vvm are applied in a pneumatic bioreactor.…”

Manipulating indole alkaloid production by Catharanthus roseus cell cultures in bioreactors: from biochemical processing to metabolic engineering

“…It was shown that high-density cell cultures gave much higher productivity of target secondary metabolites, particularly in combination with other strategies, thus corresponding bioreactor configurations have been developed for high density cultures, such as a double helicalribbon impeller reactor and maxblend fermentor (Jolicoeur et al 1992;Yokoi et al 1993;Zhong 2001;Zhao et al 2000aZhao et al , 2001a. It thus seems that high-density culture could be best for production of intracellular-accumulated secondary metabolites in combination with stimulation strategies.…”

“…A comparison of a variety of bioreactors with different agitation and aeration systems, as to their performance on biomass and secondary metabolite production of C. roseus cells showed the airlift bioreactor as the most suitable system (Misawa 1994). A double helical-ribbon impeller bioreactor with working volume of 11-l was designed for high-density C. roseus cell culture (Jolicoeur et al 1992), a Maxblend fermentor for high-density culture was developed and tested for rice and C. roseus cell cultures (Yokoi et al 1993). A biofilm reactor that contains a horizontal biofilm as a matrix to support cell cultures and circulated production medium to support living of cells was developed, showing less growth rate but being more effective in maximizing indole alkaloid titers than suspension cultures (Kargi et al 1990;Kargi and Ganapath 1991).…”