High-Density Fiber-Optic Genosensor Microsphere Array Capable of Zeptomole Detection Limits

Epstein, Jason R.; Lee, Myoyong; Walt, David R.

doi:10.1021/ac0156619

Cited by 97 publications

(110 citation statements)

References 19 publications

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“…Optical fiber microwell/microbead arrays have been applied to nucleic acid biosensing [88]. The microspheres were encoded with a fluorescent dye, functionalized with probe oligonucleotides and immobilized in microwells of etched imaging fiber.…”

Section: Sensing Schemesmentioning

confidence: 99%

Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor

et al. 2005

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(ABSTRACT)Fiber-optic Fabry-Perot interferometric (FFPI) sensors have been widely used due to their high sensitivity, ease of fabrication, miniature size, and capability for multiplexing. However, direct measurement of self-assembled thin films, receptor immobilization process or biological reaction is limited in the FFPI technique due to the difficulty of forming Fabry-Perot cavities by the thin film itself. Novel methods are needed to provide an accurate and reliable measurement for monitoring the thin-film growth in the nanometer range and under various conditions. In this work, two types of fiber-optic multicavity Fabry-Perot interferometric (MFPI) sensors with built-in temperature compensation were designed and fabricated for thin-film measurement, with applications in chemical and biological sensing. Both the tubing-based MFPI sensor and microgap MFPI sensor provide simple, yet high performance solutions for thin-film sensing. The temperature dependence of the sensing cavity is compensated by extracting the temperature information from a second multiplexed cavity. This provides the opportunity to examine the thin-film characteristics under different environment temperatures.To demonstrate the potential of this structure for practical applications, immunosensors were fabricated and tested using these structures. Self-assembled polyelectrolytes served as a precursor film for immobilization of antibodies to ensure they retain their biological activity. This not only provides a convenient method for protein immobilization but also presents the possibility of increasing the binding capacity

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Section: Sensing Schemesmentioning

confidence: 99%

Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor

et al. 2005

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“…Although some preliminary data suggested that the platform is capable of high sensitivity (Epstein et al 2002), a number of significant challenges had to be overcome in order to create a robust, quantitative, high-performance system suitable for use with biological samples such as mammalian poly(A + ) mRNA. We have now developed such a system.…”

mentioning

confidence: 99%

A novel, high-performance random array platform for quantitative gene expression profiling

Kuhn¹,

Baker²,

Chudin³

et al. 2004

Genome Res.

173

138

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We have developed a new microarray technology for quantitative gene-expression profiling on the basis of randomly assembled arrays of beads. Each bead carries a gene-specific probe sequence. There are multiple copies of each sequence-specific bead in an array, which contributes to measurement precision and reliability. We optimized the system for specific and sensitive analysis of mammalian RNA, and using RNA controls of defined concentration, obtained the following estimates of system performance: specificity of 1:250,000 in mammalian poly(A + ) mRNA; limit of detection 0.13 pM; dynamic range 3.2 logs; and sufficient precision to detect 1.3-fold differences with 95% confidence within the dynamic range. Measurements of expression differences between human brain and liver were validated by concordance with quantitative real-time PCR (R 2 = 0.98 for log-transformed ratios, and slope of the best-fit line = 1.04, for 20 genes). Quantitative performance was further verified using a mouse B-and T-cell model system. We found published reports of B-or T-cell-specific expression for 42 of 59 genes that showed the greatest differential expression between B-and T-cells in our system. All of the literature observations were concordant with our results. Our experiments were carried out on a 96-array matrix system that requires only 100 ng of input RNA and uses standard microtiter plates to process samples in parallel. Our technology has advantages for analyzing multiple samples, is scalable to all known genes in a genome, and is flexible, allowing the use of standard or custom probes in an array.

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“…Chemical etching is a commonly used technique in optical fabrication, such as nanoprobes [51], and microwells [52] . The general idea is to use hydrofluoric acid (HF) to etch the fiber tip.…”

Section: Fabricationmentioning

confidence: 99%

Microgap Structured Optical Sensor for Fast Label-Free DNA Detection

Wang

Cooper

Wang

2008

J. Lightwave Technol.

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DNA detection technology has developed rapidly due to its extensive application in clinical diagnostics, bioengineering, environmental monitoring, and food science areas.Currently developed methods such as surface Plasmon resonance (SPR) methods, fluorescent dye labeled methods and electrochemical methods, usually have the problems of bulky size, high equipment cost and time-consuming algorithms, so limiting their application for in vivo detection. In this work, an intrinsic Fabry-Perot interferometric (IFPI) based DNA sensor is presented with the intrinsic advantages of small size, low cost and corrosion-tolerance. This sensor has experimentally demonstrated its high sensitivity and selectivity.In theory, DNA detection is realized by interrogating the sensor's optical cavity length variation resulting from hybridization event. First, a microgap structure based IFPI sensor is fabricated with simple etching and splicing technology. Subsequently, considering the sugar phosphate backbone of DNA, layer-by-layer electrostatic self-assembly technique is adopted to attach the single strand capture DNA to the sensor endface. When the target DNA strand binds to the single-stranded DNA successfully, the optical cavity length of sensor will be increased. Finally, by demodulating the sensor spectrum, DNA hybridization event can be judged qualitatively.This sensor can realize DNA detection without attached label, which save the experiment expense and time. Also the hybridization detection is finished within a few minutes.This quick response feature makes it more attractive in diagnose application. Since the iii sensitivity and specificity are the most widely used statistics to describe a diagnostic test, so these characteristics are used to evaluate this biosensor. Experimental results demonstrate that this sensor has a sensitivity of 6nmol/ml and can identify a 2 bp mismatch. Since this sensor is optical fiber based, it has robust structure and small size ( 125μm ). If extra etching process is applied to the sensor, the size can be further reduced. This promises the sensor potential application of in-cell detection. Further investigation can be focused on the nanofabrication of this DNA sensor, and this is very meaningful topic not only for diagnostic test but also in many other applications such as food industry, environment monitoring.iv Acknowledgement

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High-Density Fiber-Optic Genosensor Microsphere Array Capable of Zeptomole Detection Limits

Cited by 97 publications

References 19 publications

Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor

Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor

A novel, high-performance random array platform for quantitative gene expression profiling

Microgap Structured Optical Sensor for Fast Label-Free DNA Detection

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