High-density lipoprotein subpopulation profiles in lipoprotein lipase and hepatic lipase deficiency

Abstract

Background and aims Our aim was to gain insight into the role that lipoprotein lipase (LPL) and hepatic lipase (HL) plays in HDL metabolism and to better understand LPL- and HL-deficiency states. Methods We examined the apolipoprotein (apo) A-I-, A-II-, A-IV-, C-I-, C-III-, and E-containing HDL subpopulation profiles, assessed by native 2-dimensional gel-electrophoresis and immunoblotting, in 6 homozygous and 11 heterozygous LPL-deficient, 6 homozygous and 4 heterozygous HL-deficient, and 50 control subjects. Results LPL-deficient homozygotes had marked hypertriglyceridemia and significant decreases in LDL-C, HDL-C, and apoA-I. Their apoA-I-containing HDL subpopulation profile was shifted toward small HDL particles compared to controls. HL-deficient homozygotes had moderate hypertriglyceridemia, modest increases in LDL-C and HDL-C level, but normal apoA-I concentration. HL-deficient homozygotes had a unique distribution of apoA-I-containing HDL particles. The normally apoA-I:A-II, intermediate-size (α-2 and α-3) particles were significantly decreased, while the normally apoA-I only (very large α-1, small α-4, and very small preβ-1) particles were significantly elevated. In contrast to control subjects, the very large α-1 particles of HL-deficient homozygotes were enriched in apoA-II. Homozygous LPL- and HL-deficient subjects also had abnormal distributions of apo C-I, C-III, and E in HDL particles. Values for all measured parameters in LPL- and HL-deficient heterozygotes were closer to values measured in controls than in homozygotes. Conclusions Our data are consistent with the concept that LPL is important for the maturation of small discoidal HDL particles into large spherical HDL particles, while HL is important for HDL remodeling of very large HDL particles into intermediate-size HDL particles.

Influence of HDL particles on cell-cholesterol efflux under various pathological conditions

“…High TG levels are associated with high concentrations of TG-rich lipoproteins (TRLs), which in turn are associated with enhanced LPL activity. Through the action of LPL on TRL, some lipids and apolipoproteins are released from TRLs and are transferred to HDL particles (23). Therefore, a high TG level improves not only the first step of RCT, but also the maturation of small HDL particles into larger more complex particles.…”

Association of 2-hour Plasma Glucose (2H-PG) with Lipid Profile in Selected Diabetic Families from Hyderabad Sindh Pakistan

“…The lipoprotein lipase enzyme breaks up the triglycerides in very low-density lipoprotein cholesterol so they can store inside fat tissue. Without enough lipoprotein lipase, diabetics may have a high triglyceride level 19 . The diabetics who do not control their sugar level can have a triglyceride level and can cause the pancreatic inflammation 20 .…”

Parameters of Carbohydrate Metabolism and Expression of Liver Steatosis in Patients with Nonalcoholic Fatty Liver Disease and Hypertensive Depending on Lipid Parameters

“…Изучение зависимостей концентрации ЭЛ с показателями липидограммы выявило положительную связь только с уровнем общего холестерина и отрицательныйс уровнем ЛПНП, что также было представлено в исследовании Tani M. et al [18]. Отсутствие прямых ассоциаций между уровнями ЭЛ и содержанием в крови ЛПВП может быть связано с вероятным угнетением активности липопротеинлипазы у избранного для исследования контингента и перекрестного участия ЭЛ в метаболизме ЛПНП, а так же с разным метаболическим классом исследуемых, что так же отмечено в исследовании Potočnjak I. et al [15].…”