High Detection Rates of Human Bocavirus in Infants and Small Children with Lower Respiratory Tract Infection from Croatia

Ljubin‐Sternak, Sunčanica; Meštrović, Tomislav; Ivković‐Jureković, Irena; Tešović, Goran; Mlinarić‐Galinović, Gordana; Lukšić, Ivana; Tabain, Irena; Tot, Tatjana; Mijač, Maja; Vraneš, Jasmina

doi:10.7754/clin.lab.2018.180702

Cited by 12 publications

(11 citation statements)

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“…Competition or interference between viruses in the respiratory tract could explain the low viral loads observed in the majority of coinfections. In our study, HBoV viral loads were comparable between the monoinfected and coinfected samples, as was also found in previous studies [7,23,30]. However, the small number of samples in the monoinfected group makes it difficult to prove any statistical difference between the two.…”

Section: Discussionsupporting

confidence: 87%

“…This child showed persistent HBoV PCR positivity in seven separate determinations (6 in monoinfection, 1 in coinfection). The other three premature infants expressed HBoV at higher Cq values (30)(31)(32)(33)(34) and had only mild deterioration of their respiratory condition. A child born at a gestational age of 26 weeks had severe BPD with a need for invasive ventilation (conventional and high-frequency oscillation) for 33 days followed by 18 days of CPAP.…”

Section: Nosocomial Monoinfectionmentioning

confidence: 93%

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Human bocavirus infection in Belgian children with respiratory tract disease

et al. 2019

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Human bocavirus (HBoV) has been detected primarily in children with acute lower respiratory tract disease (LRTD), but its occurrence, clinical profile, and role as a causative agent of RTD are not clear. The aim of this study was to investigate the prevalence and the potential clinical relevance of HBoV. Using molecular tests, we tested 1352 nasopharyngeal samples obtained between October 1, 2017 and April 30, 2018 from children up to the age of 16 with RTD for the presence of HBoV DNA and 20 other respiratory pathogens at three different hospitals in Belgium. HBoV was detected in 77 children with a median age of 10.6 months. Consecutive samples were available for 15 HBoV-positive children and showed persistent HBoV positivity in four of them. Monoinfection was observed in six infants. Four of them were born prematurely and were infected during hospitalization at the neonatal intensive care unit (NICU). Only one of these six monoinfected children was diagnosed with recurrent wheezing due to HBoV. This child was carried to term and had a high viral load. Coinfections, most frequently with rhinovirus (52.1%) and adenovirus (49.3%), were observed in 72 patients. In seventeen of them in which HBoV was present at high viral load or higher viral load than its copathogens, bronchi(oli)tis (n = 8), recurrent wheezing (n = 8) or episodic wheezing (n = 1) were diagnosed. Our results suggest that HBoV infection at high viral load in infants is associated with wheezing (P = 0.013, Cramer's V = 0.613). Abbreviations BPDBronchopulmonary dysplasia CLD Chronic lung disease CPAP Continuous positive airway pressure Cq Quantitation cycle CRP C-reactive protein EV Enterovirus GI Gastrointestinal HAdV

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Section: Discussionsupporting

confidence: 87%

Section: Nosocomial Monoinfectionmentioning

confidence: 93%

Human bocavirus infection in Belgian children with respiratory tract disease

et al. 2019

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“…The frequency of HBoV co-detection is in line with Zhou et al (52%) and Ljubin-Sternak et al [2,17], higher than the one found by Sun et al [18] and only a bit lower than other studies [5,7,19]. In infants hospitalized for an acute respiratory infection, Calvo et al in one study detected HBoV in combination with other viruses in 64.6% of children and in another study in 75% of the patients [5,19].…”

Section: Discussionsupporting

confidence: 80%

“…Human bocavirus (HBoV), a new virus belonging to the Parvoviridae family, was first identified in 2005 in respiratory samples of children suffering from viral respiratory infections of unknown etiology [1]. Several studies tried to investigate the role of HBoV, reporting a detection rate ranging from 3.1% [1] to 23.1% [2] in children with acute respiratory tract infections. Sometimes, HBoV was considered responsible for a severe clinical presentation in children with acute respiratory disease [3]; however, other studies reported no differences between HBoV and other viruses in the clinical course of respiratory infections [4].…”

Section: Introductionmentioning

confidence: 99%

Human bocavirus in children hospitalized for acute respiratory tract infection in Rome

et al. 2019

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Background The role of human bocavirus (HBoV) as a respiratory pathogen has not been fulfilled yet. We aimed to describe clinical and serological characteristics of children with HBoV hospitalized for acute respiratory tract infection and to evaluate whether differences occur between HBoV alone and in co-infection. Methods We retrospectively reviewed data from 60 children (median age of 6.2 months, range 0.6-70.9) hospitalized for acute respiratory symptoms, with HBoV detected from a respiratory sample, using a reverse transcriptase-PCR for 14 respiratory viruses (including respiratory syncytial virus (RSV), influenza virus A and B, human coronavirus OC43, 229E, NL-63 and HUK1, adenovirus, rhinovirus, parainfluenza virus1-3, and human metapneumovirus).Results HBoV was detected alone in 29 (48.3%) patients, while in co-infection with other viruses in 31 patients (51.7%), with a peak between December and January. Among the 60 patients, 34 were bronchiolitis, 19 wheezing, 3 pneumonia, 2 upper respiratory tract infection, and 2 whooping cough. Seven children (11.6%) required admission to the paediatric intensive care unit (PICU) for respiratory failure. No differences was observed in age, family history for atopy and/or asthma, clinical presentations, chest X-ray, or laboratory findings in children with HBoV alone vs. multiple viral detection. RSV was the most frequently co-detected virus (61.3%). When compared with HBoV detection alone, the co-detection of RSV and HBoV was associated with male sex (P = 0.013), younger age (P = 0.01), and lower blood neutrophil count (P = 0.032). Conclusions HBoV can be detected alone and in co-infection respiratory samples of children with an acute respiratory tract infection. A cause-effect relationship between HBoV and respiratory infection is not clear, so further studies are needed to clarify this point.the cause-effect relationship between HBoV detection and respiratory infections cannot be achieved, yet based on our data and further studies are needed to clarify if HBoV can play a pathogenetic role in respiratory diseases of children.

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“…The detection of HBoV co-infection with other viruses in patients with respiratory diseases has been demonstrated in many studies. In these studies, the rate of HBoV co-infection ranged from 17.6–84.4 % [31–34]. The study by Sloots et al .…”

Section: Resultsmentioning

confidence: 99%

Human bocavirus (HBoV) in Kuwait: molecular epidemiology and clinical outcome of the virus among patients with respiratory diseases

Madi

Al-Adwani

2020

Journal of Medical Microbiology

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Introduction. Globally, human bocavirus (HBoV) has been detected in respiratory samples from patients suffering from upper and lower respiratory diseases. In Kuwait, little is known about the epidemiological and clinical characterization of the virus and genetic characterization of the virus as a respiratory pathogen is unknown. Aim. This study aims to explore the molecular epidemiology and clinical features of HBoV isolates in patients with respiratory diseases. Methodology. Retrospectively, between 2018 and 2020, 5941 respiratory samples from patients with respiratory diseases were screened for respiratory viruses using multiplex real-time PCR. Samples that were positive for HBoV were then subjected to NP1 and VP1/PV2 phylogenetic analysis. Results. HBoV was detected in 1.9 % of the patients, with a peak incidence of infection among children <1 year old. Co-infection with other respiratory viruses was observed in 56.8 % of HBoV-positive patients. Fever, cough and respiratory distress were the most common clinical features of HBoV infection. Phylogenetic analysis of the Kuwaiti HBoV isolates revealed that all the isolates were of the HBoV-1 genotype, with slight sequence variations among the isolates. Conclusion. This study illustrated the predominance of the HBoV-1 genotype in patients with respiratory diseases in Kuwait with minimal genetic variability. It also highlighted the clinical features of HBoV-1 infection, verifying its role in respiratory diseases.

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High Detection Rates of Human Bocavirus in Infants and Small Children with Lower Respiratory Tract Infection from Croatia

Cited by 12 publications

References 0 publications

Human bocavirus infection in Belgian children with respiratory tract disease

Human bocavirus infection in Belgian children with respiratory tract disease

Human bocavirus in children hospitalized for acute respiratory tract infection in Rome

Human bocavirus (HBoV) in Kuwait: molecular epidemiology and clinical outcome of the virus among patients with respiratory diseases

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