High Dietary Fructose Intake on Cardiovascular Disease Related Parameters in Growing Rats

Abstract

The objective of this study was to determine the effects of a high-fructose diet on cardiovascular disease (CVD)-related parameters in growing rats. Three-week-old female Sprague Dawley rats were randomly assigned to four experimental groups; a regular diet group (RD: fed regular diet based on AIN-93G, n = 8), a high-fructose diet group (30Frc: fed regular diet with 30% fructose, n = 8), a high-fat diet group (45Fat: fed regular diet with 45 kcal% fat, n = 8) or a high fructose with high-fat diet group (30Frc + 45Fat, fed diet 30% fructose with 45 kcal% fat, n = 8). After an eight-week treatment period, the body weight, total-fat weight, serum glucose, insulin, lipid profiles and pro-inflammatory cytokines, abdominal aortic wall thickness, and expressions of eNOS and ET-1 mRNA were analyzed. The result showed that total-fat weight was higher in the 30Frc, 45Fat, and 30Frc + 45Fat groups compared to the RD group (p < 0.05). Serum triglyceride (TG) levels were highest in the 30Frc group than the other groups (p < 0.05). The abdominal aorta of 30Frc, 45Fat, and 30Frc + 45Fat groups had higher wall thickness than the RD group (p < 0.05). Abdominal aortic eNOS mRNA level was decreased in 30Frc, 45Fat, and 30Frc + 45Fat groups compared to the RD group (p < 0.05), and also 45Fat and 30Frc + 45Fat groups had decreased mRNA expression of eNOS compared to the 30Frc group (p < 0.05). ET-1 mRNA level was higher in 30Frc, 45Fat, and 30Frc + 45Fat groups than the RD group (p < 0.05). Both high fructose consumption and high fat consumption in growing rats had similar negative effects on CVD-related parameters.

Curcumin ameliorates protein expression changes involved in mitochondrial fatty acids metabolism in heart of mice fed a high-fructose diet

“…In the present study, the HFD after 15 weeks, did not induce changes in glucose concentration, so we did not expect an effect of curcumin. The same effect was reported by Yoo et al [22]; where the administration of 30% fructose for 20 weeks does not induce changes in blood glucose. Another study reported that the HFD induced body weight gain in rats, while curcumin supplementation prevented an increase in fructose-induced body weight [29].…”

“…Curcumin group (C+Cur), Fructose group (F) and Fructose + Curcumin group (F+Cur). F groups were administered with 30% (w/v) fructose in the water [22] and Cur groups were administered with 0.75% (w/w) curcumin in the diet [20]. At the end of the treatment, the mice fasted for 8 h (after weighing), subsequently were sacrificed by decapitation, then the heart was removed and blood sample was obtained for measure serum glucose and cholesterol concentration, through a commercial kit (Spinreact, SA, Girona, Spain).…”

The protective effects of Δ9-tetrahydrocannabinol against inflammation and oxidative stress in rat liver with fructose-induced hyperinsulinemia

“…[29,30] According to the study of Wu et al [31] , blood TG increased in rats received 60% fructose in food source during 6 weeks as compared to control group. Yoo et al [32] reported that serum TG level was increased, but not serum HDL and TC by 30% fructose for 8 weeks. Likely, our data showed that serum VLDL and TG levels increased with liquid fructose % 10 in for 12 weeks but TC and HDL did not change.…”

“…blood TG increased in rats received 60% fructose in food source during 6 weeks as compared to control group. Yoo et al . reported that serum TG level was increased, but not serum HDL and TC by 30% fructose for 8 weeks.…”

Blood Brain Barrier Injury in Diabetes: Unrecognized Effects on Brain and Cognition

“…Occludin expression was decreased in two rat models of DM (Persidsky et al, 2016; Yoo et al, 2016b). A human monoculture study (Argaw et al, 2009) replicated this finding, while also observing decreased claudin-5 and no effect on ZO-1.…”