High-dimensional biology in obstetrics and gynecology: Functional genomics in microarray studies

Romero, Roberto; Tromp, Gerard

doi:10.1016/j.ajog.2006.06.077

Cited by 33 publications

(16 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By prospectively collecting salivary samples from a new group of neonates, we validated that assumption and demonstrated that there is a distinct gene expression profile associated with neonatal transitioning at birth that can be seen independently, repeatedly, and in different biofluids. Indeed, Romero and Tromp have argued that validation of micro-array experiments must be conducted in different sets of samples, ‘otherwise the validation will be of the microarray technique, rather than the biological problem under study’ [10]. …”

Section: Discussionmentioning

confidence: 99%

“…Informed parental consent was obtained for each enrolled subject ( n = 20). There is a growing consensus in the literature that a minimum of five microarray experiments are required for statistically significant results [10]. For the purpose of this study, we chose to exceed those standards and analyze 10 biological specimens collected from two groups of neonates.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Performing discovery-driven neonatal research by transcriptomic analysis of routinely discarded biofluids

Maron¹,

Dietz²,

Parkin³

et al. 2012

The Journal of Maternal-Fetal & Neonatal Medicine

View full text Add to dashboard Cite

Objective To perform discovery-driven research on the neonatal salivary and cord blood transcriptomes. Methods Two separate cohorts of infants were enrolled in this study. In one, cord blood (n = 10) and in the other, saliva samples (n = 10) were collected at term gestation. Total RNA was extracted, amplified and hybridized onto Affymetrix HG U133a gene expression microarrays. Following normalization, genes expressed in the highest quintile (≥ 80%) across all subjects in each biofluid were analyzed with Ingenuity Pathway Analysis. Over-represented pathways relating to organ specific development and physiological functions in the newborn were explored. Results There were 303 genes in neonatal saliva and 282 genes in umbilical cord blood that met statistical criteria. Of these, 114 were common to both biofluids. Pathway analyses revealed the important roles of redox balance, cellular proliferation, and smooth muscle relaxation. In blood, hematopoiesis and immune response pathways predominated. In saliva, pathways associated with the gastrointestinal system were highlighted. Conclusions Neonatal cord blood and saliva provide a wealth of transcriptomic information. These normally discarded biofluids should be considered an important source of real-time gene expression data that may elucidate key pathways in neonatal physiology and pathology.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Performing discovery-driven neonatal research by transcriptomic analysis of routinely discarded biofluids

Maron¹,

Dietz²,

Parkin³

et al. 2012

The Journal of Maternal-Fetal & Neonatal Medicine

View full text Add to dashboard Cite

show abstract

“…3 Several reviews on the application of these technologies to reproductive medicine have recently been published. [4][5][6][7][8][9] Application to the endometrium is presented herein.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomics

Giudice¹,

Talbi²,

Hamilton³

et al. 2008

Reproductive Medicine and Assisted Reproductive Techniques

View full text Add to dashboard Cite

“…Metabolomics and other high-dimensional biology approaches (genomics, transcriptomics, metabolomics, lipidomics, glycomics, etc.) [2,3] represent discovery platforms to identify differences in the composition of biological samples (e.g. serum or plasma, urine, cerebrospinal fluid, amniotic fluid, tissues, etc.)…”

Section: Author's Replymentioning

confidence: 99%