High diversity of genetic lineages and virulence genes in nasal Staphylococcus aureusisolates from donkeys destined to food consumption in Tunisia with predominance of the ruminant associated CC133 lineage

Gharsa, Haythem; Sallem, Rym Ben; Slama, Karim Ben; Gómez-Sanz, Elena; Lozano, Carmen; Jouini, Ahlem; Klibi, Naouel; Zarazaga, Myriam; Boudabous, Abdellatif; Torres, Carmén

doi:10.1186/1746-6148-8-203

Cited by 43 publications

(42 citation statements)

References 44 publications

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“…The carriage of S. aureus has been evaluated revealing that S. aureus colonization is common in free-living artiodactyls (Table 1). However, the carriage rates detected in the present study are lower than those reported in different domestic animals such as pigs (36%), small ruminants (from 29 to 64%), donkeys (50%), and rabbits (56%) (26)(27)(28)(29)(30)(31). In contrast to the results obtained for MRSA (17), the detection rate of MSSA in the Eurasian griffon vulture was lower than in the Iberian ibex, red deer, and wild boar, although the differences were not significant (P ϭ 0.057).…”

Section: Discussioncontrasting

confidence: 88%

“…The highest resistance percentage found in our study was against benzylpenicillin (22.17%; 95% CI ϭ 16.81 to 27.54%). Although the origin of this resistance is unknown, previous studies showed that it is broadly disseminated in food animals (20,26,27) and humans (39)(40)(41). None of the ST1 and ST398 MSSA isolates exhibited the antimicrobial susceptibility patterns observed in MRSA (tetracycline resistance in ST398 and ciprofloxacin susceptibility plus clindamycin-erythromycin-tetracycline resistance in ST1 [17]).…”

Section: Discussionmentioning

confidence: 98%

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Carriage of Staphylococcus aureus by Free-Living Wild Animals in Spain

Porrero

Mentaberre

Sánchez

et al. 2014

Appl Environ Microbiol

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eThe presence of methicillin-susceptible Staphylococcus aureus (MSSA) was analyzed in different free-living wild animals to assess the genetic diversity and predominant genotypes on each animal species. Samples were taken from the skin and/or nares, and isolates were characterized by spa typing, multilocus sequence typing (MLST) and antimicrobial susceptibility testing. The proportion of MSSA carriers were 5.00, 22.93, 19.78, and 17.67% in Eurasian griffon vulture, Iberian ibex, red deer, and wild boar, respectively (P ‫؍‬ 0.057). A higher proportion of isolates (P ‫؍‬ 0.000) were recovered from nasal samples (78.51%) than skin samples (21.49%), but the 9.26% of red deer and 18.25% of wild boar would have been undetected if only nasal samples had been tested. Sixty-three different spa types were identified, including 25 new spa types. The most common were t528 (43.59%) in Iberian ibex, t548 and t11212 (15.79% and 14.04%) in red deer, and t3750 (36.11%) in wild boar. By MLST, 27 STs were detected, of which 12 had not been described previously. The most frequent were ST581 for Iberian ibex (48.72%), ST425 for red deer (29.82%), and ST2328 for wild boar (42.36%). Isolates from Eurasian griffon vulture belong to ST133. Host specificity has been observed for the most frequent spa types and STs (P ‫؍‬ 0.000). The highest resistance percentage was found against benzylpenicillin (average, 22.2%), although most of the S. aureus isolates were susceptible to all antimicrobial tested. Basically, MSSA isolates were different from those MRSA isolates previously detected in the same animal species.

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Section: Discussioncontrasting

confidence: 88%

Section: Discussionmentioning

confidence: 98%

Carriage of Staphylococcus aureus by Free-Living Wild Animals in Spain

Porrero

Mentaberre

Sánchez

et al. 2014

Appl Environ Microbiol

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“…This strain was also frequently observed in small domestic ruminants, i . e ., goats and sheep [2,3,154], but also in domestic pigs [123], cats, dogs, [156], cattle [155] and donkeys [157]. It could have been transmitted from wild to domestic ruminants, or vice versa , and the few reported human cases [116] could be related to contact to animals or animal products.…”

Section: Resultsmentioning

confidence: 99%

Diversity of Staphylococcus aureus Isolates in European Wildlife

et al. 2016

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Staphylococcus aureus is a well-known colonizer and cause of infection among animals and it has been described from numerous domestic and wild animal species. The aim of the present study was to investigate the molecular epidemiology of S. aureus in a convenience sample of European wildlife and to review what previously has been observed in the subject field. 124 S. aureus isolates were collected from wildlife in Germany, Austria and Sweden; they were characterized by DNA microarray hybridization and, for isolates with novel hybridization patterns, by multilocus sequence typing (MLST). The isolates were assigned to 29 clonal complexes and singleton sequence types (CC1, CC5, CC6, CC7, CC8, CC9, CC12, CC15, CC22, CC25, CC30, CC49, CC59, CC88, CC97, CC130, CC133, CC398, ST425, CC599, CC692, CC707, ST890, CC1956, ST2425, CC2671, ST2691, CC2767 and ST2963), some of which (ST2425, ST2691, ST2963) were not described previously. Resistance rates in wildlife strains were rather low and mecA-MRSA isolates were rare (n = 6). mecC-MRSA (n = 8) were identified from a fox, a fallow deer, hares and hedgehogs. The common cattle-associated lineages CC479 and CC705 were not detected in wildlife in the present study while, in contrast, a third common cattle lineage, CC97, was found to be common among cervids. No Staphylococcus argenteus or Staphylococcus schweitzeri-like isolates were found. Systematic studies are required to monitor the possible transmission of human- and livestock-associated S. aureus/MRSA to wildlife and vice versa as well as the possible transmission, by unprotected contact to animals. The prevalence of S. aureus/MRSA in wildlife as well as its population structures in different wildlife host species warrants further investigation.

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“…Strains used in this study were isolated in the course of previous and on-going studies3940 or collected as part of routine surveillance. Genomic DNA was extracted with the MasterPure Gram-positive DNA purification kit (Cambio, United Kingdom).…”

Section: Methodsmentioning

confidence: 99%

Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus

Koop

Vrieling

Storisteanu

et al. 2017

Sci Rep

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Bicomponent pore-forming leukocidins are a family of potent toxins secreted by Staphylococcus aureus, which target white blood cells preferentially and consist of an S- and an F-component. The S-component recognizes a receptor on the host cell, enabling high-affinity binding to the cell surface, after which the toxins form a pore that penetrates the cell lipid bilayer. Until now, six different leukocidins have been described, some of which are host and cell specific. Here, we identify and characterise a novel S. aureus leukocidin; LukPQ. LukPQ is encoded on a 45 kb prophage (ΦSaeq1) found in six different clonal lineages, almost exclusively in strains cultured from equids. We show that LukPQ is a potent and specific killer of equine neutrophils and identify equine-CXCRA and CXCR2 as its target receptors. Although the S-component (LukP) is highly similar to the S-component of LukED, the species specificity of LukPQ and LukED differs. By forming non-canonical toxin pairs, we identify that the F-component contributes to the observed host tropism of LukPQ, thereby challenging the current paradigm that leukocidin specificity is driven solely by the S-component.

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High diversity of genetic lineages and virulence genes in nasal Staphylococcus aureusisolates from donkeys destined to food consumption in Tunisia with predominance of the ruminant associated CC133 lineage

Cited by 43 publications

References 44 publications

Carriage of Staphylococcus aureus by Free-Living Wild Animals in Spain

Carriage of Staphylococcus aureus by Free-Living Wild Animals in Spain

Diversity of Staphylococcus aureus Isolates in European Wildlife

Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus

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