High DNA melting activity of extremophyte Eutrema salsugineum cold shock domain proteins EsCSDP1 and EsCSDP3

Zlobin, Nikolay; Evlakov, Konstantin; Alekseev, Ya. I.; Blagodatskikh, Konstantin A.; Бабаков, А. В.; Taranov, Vasiliy

doi:10.1016/j.bbrep.2016.02.004

Cited by 2 publications

(10 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The main RNA chaperone protein of E. coli CspA did not show substantial melting activity against BC1rna beacon. At the same time, we have previously established that CspA melted DNA beacon BC1 [15]. Presumably, the low melting activity of CspA against BC1rna is due to the higher Tm of BC1rna beacon in comparison with BC1 beacon (52 о С compared to approximately 30 о С, respectively).…”

Section: Resultsmentioning

confidence: 89%

“…S1). This beacon is structurally identical to the beacon BC1 previously used for the investigation of plant CSDP DNA melting activity [8,15]. However, it contains ribonucleotides, rather than deoxyribonucleotides, and uracil, rather than, thymine.…”

Section: Resultsmentioning

confidence: 98%

“…S2). EsCSDP3mut protein, which contains point mutations in RNA binding motifs (three in CSD and one in each zinc finger, see ref [15].) did not exhibit any RNA melting activity.…”

Section: Resultsmentioning

confidence: 98%

“…The procedures for the generation of the genetic constructs for the expression of EsCSDP1-3, the individual CSDs of these molecules, the C-terminal fragment from EsCSDP1, EsCSDP3mut and CspA are described in detail elsewhere [15]. To amplify the genes coding the truncated variants of the EsCSDP1 protein (Supplementary 1 Figure S4), the primers listed in Supplementary 1 Table S1 were used.…”

Section: Genetic Constructs and Recombinant Protein Preparationmentioning

confidence: 99%

“…The genetic constructs for the expression of Es1(4Z) and Es1 (2Z) proteins were created using the same approach as EsCSDP1expressing genetic constructs. Isolation and purification of proteins were performed as previously described [15]. The purity of proteins was determined using PAAG-electrophoresis and LC-MS/MS analysis (Supplementary Material 2).…”

Section: Genetic Constructs and Recombinant Protein Preparationmentioning

confidence: 99%

See 4 more Smart Citations

RNA melting and RNA chaperone activities of plant cold shock domain proteins are not correlated

et al. 2018

Self Cite

View full text Add to dashboard Cite

Cold shock domain proteins (CSDPs) participate in plant development and resistance, but the underlying molecular mechanisms are poorly understood. In this study, we demonstrated that the CSDPs, including EsCSDP1, EsCSDP2, and EsCSDP3, from the extremophyte Eutrema salsugineum possess all basic properties of RNA chaperones. EsCSDP1-3 melt secondary structures in RNAs with various nucleotide sequences and exhibit RNA chaperone activity in vitro. EsCSDP1 and EsCSDP3 were shown to have higher RNA melting activity, whereasile EsCSDP2 had higher RNA chaperone activity. We demonstrated that higher RNA melting activity correlates with the longer C-terminal fragment in many zinc finger motifs, whereas increased RNA chaperone activity was most likely due to the higher glycine content and RGG repeat number in the C-terminal fragment.

show abstract

Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 98%

“…S2). EsCSDP3mut protein, which contains point mutations in RNA binding motifs (three in CSD and one in each zinc finger, see ref [15].) did not exhibit any RNA melting activity.…”

Section: Resultsmentioning

confidence: 98%

Section: Genetic Constructs and Recombinant Protein Preparationmentioning

confidence: 99%

Section: Genetic Constructs and Recombinant Protein Preparationmentioning

confidence: 99%

See 3 more Smart Citations

RNA melting and RNA chaperone activities of plant cold shock domain proteins are not correlated

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

Genes with Cold Shock Domain from Eutrema salsugineum (Pall.) for Generating a Cold Stress Tolerance in Winter Rape (Brassica napus L.) Plants

et al. 2021

Self Cite

View full text Add to dashboard Cite

The increased demand in vegetable oil for food purposes and high-protein feed for livestock and poultry encourages producers to expand the production of various oil crops, while occupying rather cold agroclimatic zones. Improved cold and frost resistance of cultivated crops would significantly increase the yield and expand the range of rape cultivation in a number of cold climate regions. Nine transgenic lines of winter rape containing genes encoding proteins with a cold shock domain (CspA и EsCSDP3) were obtained as a result of Agrobacterium transformation. In total, 260 explants were involved in transformation of rape using pBI121-CSPA-plant, with a transformation efficiency of 2.3%; among 750 explants using the pBI-EsCSDP3 construction, the efficiency was 0.4%. As a result of the studies, it was shown that the expression of the new gene Escsdp3 from the plant of Eutrema salsugineum was able to increase the cold and frost resistance of plants as effectively as the cspa gene from E. coli, which is classically used for this purpose. The cold resistance analysis of T1 transgenic plants generation revealed four cold resistant winter rape lines (three lines with the cspA-plant gene and one line with the Escsdp3 gene). The transfer of Escsdp3 and cspA-plant genes into winter rape plants led to a significant increase in frost resistance of plants. Two winter rapeseed lines were resistant to freezing (with the cspA-plant gene and with the Escsdp3 gene). Non-hardened transgenic plants remained viable after 24 h of exposure to negative temperatures up to −5 °C, and plants that passed through the hardening stage survived after freezing at −16 °C.

show abstract

High DNA melting activity of extremophyte Eutrema salsugineum cold shock domain proteins EsCSDP1 and EsCSDP3

Cited by 2 publications

References 29 publications

RNA melting and RNA chaperone activities of plant cold shock domain proteins are not correlated

RNA melting and RNA chaperone activities of plant cold shock domain proteins are not correlated

Genes with Cold Shock Domain from Eutrema salsugineum (Pall.) for Generating a Cold Stress Tolerance in Winter Rape (Brassica napus L.) Plants

Contact Info

Product

Resources

About