High dose concentration administration of ascorbic acid inhibits tumor growth in BALB/C mice implanted with sarcoma 180 cancer cells via the restriction of angiogenesis

Yeom, Chang‐Hwan; Lee, Gunsup; Park, Jin Hee; Yu, Jae Sik; Park, Sung Yong; Yi, Sang-Yeop; Lee, Hye Ree; Hong, Young Seon; Yang, Joo-Sung; Lee, Sukchan

doi:10.1186/1479-5876-7-70

Cited by 71 publications

(48 citation statements)

References 36 publications

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“…In addition, AsA has been used as an agent for anti-oxidant therapy (Wang and Yi 2008). High-dose AsA administered to Balb/c mice implanted with sarcoma 180 cancer cells has been shown to suppress genes involved in angiogenesis (Yeom et al 2009). In their study, expression of basic fibroblast growth factor, vascular endothelial growth factor and MMP-2 genes was greatly suppressed by AsA.…”

Section: Erw Inhibits the Invasion Of Ht1080 Cellsmentioning

confidence: 99%

Suppressive effects of electrochemically reduced water on matrix metalloproteinase-2 activities and in vitro invasion of human fibrosarcoma HT1080 cells

Kinjo

Yan

et al. 2012

Cytotechnology

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It has been demonstrated that hydrogen peroxide (H(2)O(2)) is directly associated with elevated matrix metalloproteinase-2 (MMP-2) expression in several cell lines. Electrochemically reduced water (ERW), produced near the cathode during electrolysis, and scavenges intracellular H(2)O(2) in human fibrosarcoma HT1080 cells. RT-PCR and zymography analyses revealed that when HT1080 cells were treated with ERW, the gene expression of MMP-2 and membrane type 1 MMP and activation of MMP-2 was repressed, resulting in decreased invasion of the cells into matrigel. ERW also inhibited H(2)O(2)-induced MMP-2 upregulation. To investigate signal transduction involved in MMP-2 downregulation, mitogen-activated protein kinase (MAPK)-specific inhibitors, SB203580 (p38 MAPK inhibitor), PD98059 (MAPK/extracellular regulated kinase kinase 1 inhibitor) and c-Jun NH(2)-terminal kinase inhibitor II, were used to block the MAPK signal cascade. MMP-2 gene expression was only inhibited by SB203580 treatment, suggesting a pivotal role of p38 MAPK in regulation of MMP-2 gene expression. Western blot analysis showed that ERW downregulated the phosphorylation of p38 both in H(2)O(2)-treated and untreated HT1080 cells. These results indicate that the inhibitory effect of ERW on tumor invasion is due to, at least in part, its antioxidative effect.

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Section: Erw Inhibits the Invasion Of Ht1080 Cellsmentioning

confidence: 99%

Suppressive effects of electrochemically reduced water on matrix metalloproteinase-2 activities and in vitro invasion of human fibrosarcoma HT1080 cells

Kinjo

Yan

et al. 2012

Cytotechnology

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“…N-acetyl cysteine and selenium have been observed to inhibit MMP-9 and invasive activities of tumor cells, as well as migration of endothelial cells through ECM (27)(28)(29)(30)(31). Ascorbic acid has been demonstrated to inhibit tumor growth via anti-angiogenic activity (32) and to inhibit cell division and growth in vitro through production of hydrogen peroxide (33). Arginine is a precursor of nitric oxide (NO); any deficiency of arginine can limit the production of NO, which has been shown to predominantly act as an inducer of apoptosis, as in breast cancer cells (34).…”

Section: Introductionmentioning

confidence: 99%

Comparative effects of EGCG, green tea and a nutrient mixture on the patterns of MMP-2 and MMP-9 expression in cancer cell lines

Roomi

Monterrey²,

Kalinovsky³

et al. 2010

Oncol Rep

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Abstract. Type IV collagenase matrix metalloproteinases (MMPs), especially MMP-2 and MMP-9, have been found to promote invasion and metastasis of malignant tumors. Extracellular matrix (ECM) degradation by MMPs and increased expression of MMPs in cancer cells and tumor microvascular endothelial cells make MMPs an attractive target for cancer. Focused on a common pathomechanism of cancer growth and invasion, the disintegration of connective tissue, we used natural approaches to increase the integrity and strength of connective tissues. Utilizing the principle of nutrition synergy, we developed a novel micronutrient mixture (NM) containing lysine, proline, ascorbic acid and green tea extract. This study evaluates the potency of the components EGCG and green tea extract independently compared to that of NM on modulation of patterns of MMP-2 and MMP-9 expression in four cancer cell lines expressing MMP-2, MMP-9 or both. Human fibrosarcoma (HT-1080), hepatocellular carcinoma (SKHep-1), glioblastoma (T-98G), uterine leiomyosarcoma (SK-UT-1) cell lines were obtained from ATCC and grown in minimum essential medium (MEM) supplemented with 10% FBS, penicillin (100 U/ml) and streptomycin (100 mg/ml) in 24-well tissue culture plates. At near confluence, the cells were treated with agents dissolved in media and tested at concentrations indicated in triplicate at each dose. Cells were also treated with PMA 100 ng/ml to study enhanced expression of MMP-9. MMP expression was assessed by gelatinase zymography. Fibrosarcoma and hepatocellular carcinoma cells expressed both MMP-2 and MMP-9. Glioblastoma cells expressed MMP-2 and PMA treatment induced MMP-9 expression. Uterine leimyosarcoma cells expressed no MMPs but PMA induced MMP-9. NM was the most potent dosedependent inhibitor of MMPs, followed by green tea extract and EGCG. In conclusion, these results suggest the enhanced efficacy of nutrients working in synergy to modulate complex pathways such as MMP expression.

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“…It allows to collagen formation and stability, it also has other various physiological and therapeutic effects [80,[82][83][84][85][86][87][88] including the stimulation of human fibroblasts [82]. Interestingly, systemic application of high dose of L-AA inhibited tumor growth via reduction of angiogenesis [87,88].…”

Section: Ascorbic Acid Based Polyurethane Systems For Tementioning

confidence: 99%

Ascorbic Acid in Polyurethane Systems for Tissue Engineering

Kucińska-Lipka¹,

St.²,

Janik³

et al. 2016

ChChT

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Abstract. The introduction of the paper was devoted to the main items of Tissue Engineering (TE) and the way of porous structure obtaining as scaffolds. Furthermore, the significant role of the scaffold design in TE was described. It was shown, that properly designed polyurethanes (PURs) find application in TE due to the proper physicochemical, mechanical and biological properties. Then the use of L-ascorbic acid (L-AA) in PUR systems for TE was described. L-AA has been applied in this area due to its suitable biological characteristics and antioxidative properties. Moreover, L-AA influences tissue regeneration due to improving collagen synthesis, which is a primary component of the extracellular matrix (ECM). Modification of PUR with L-AA leads to the materials with higher biocompatibility and such system is promising for TE applications.

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High dose concentration administration of ascorbic acid inhibits tumor growth in BALB/C mice implanted with sarcoma 180 cancer cells via the restriction of angiogenesis

Cited by 71 publications

References 36 publications

Suppressive effects of electrochemically reduced water on matrix metalloproteinase-2 activities and in vitro invasion of human fibrosarcoma HT1080 cells

Suppressive effects of electrochemically reduced water on matrix metalloproteinase-2 activities and in vitro invasion of human fibrosarcoma HT1080 cells

Comparative effects of EGCG, green tea and a nutrient mixture on the patterns of MMP-2 and MMP-9 expression in cancer cell lines

Ascorbic Acid in Polyurethane Systems for Tissue Engineering

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