High-dose enoxaparin in the treatment of abdominal angiostrongyliasis in Swiss mice

Sandri, Alberto; Rodriguez, Rubens; Costa, Márcio Machado; Porto, Sérgio Machado; Schwingel, Daniela; Vieira, Maria Isabel Botelho

doi:10.1017/s0022149x17000852

Cited by 5 publications

(2 citation statements)

References 11 publications

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“…The preparations and histopathological analyses were performed according to the methodology previously described by our team (Sandri et al , 2018). Macroscopic evaluation to identify anatomical alterations, such as the presence of ischaemic lesions and formation of pseudotumours, was performed in the animals during necropsies.…”

Section: Methodsmentioning

confidence: 99%

Relationship between histopathological lesions and oxidative stress in mice infected with Angiostrongylus costaricensis

et al. 2023

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This study describes changes in oxidative stress (OS) parameters in mice experimentally infected with Angiostrongylus costaricensis, which causes abdominal angiostrongyliasis. For this, 28 Swiss mice were used, divided into two groups (G1 and G2), with 14 animals each. Of these, eight were infected with ten infective larvae each, by gavage, and six were used as a control group. Mice from G1 and G2 were euthanized at 14 days and 24 days post-infection, respectively. Tissue samples were used for histopathological analysis and blood (serum) samples were taken to assess the levels of proteins, non-protein thiols (NPTs) and nitric oxide (NO), from centrifugation and subsequent collection of aliquots of the supernatant. Among OS parameters, infected mice in both groups had higher NO levels than the control group, due to the presence of: eosinophil infiltrate in the liver and intestine; pancreatitis; and intestinal granuloma. However, the infected mice of both groups showed a reduction in the levels of NPTs, in relation to the control group, due to the presence of: eosinophilic infiltrate in the liver and intestine; and intestinal granuloma. Our results suggest that A. costaricensis infection has important effects on the intestine, liver and pancreas, and the analyses were performed from the tissue of these organs. The mechanisms for these changes are related to the decrease in the body's main antioxidant defences, as demonstrated by the reduction of NPTs, thus contributing to the development of more severe tissue damage. Thus, the objective of the present study was to evaluate the relationship between histopathological lesions and markers for OS.

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Section: Methodsmentioning

confidence: 99%

Relationship between histopathological lesions and oxidative stress in mice infected with Angiostrongylus costaricensis

et al. 2023

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“…This disease is a public health problem in Latin America, especially in Costa Rica and Brazil [27]. Currently, no drugs acting directly on this parasite are available, primarily due to the sparse understanding of its molecular characteristics [28]. The current publicly available genome draft of A. costaricensis is specific to the Costa Rica strain, and its annotation is not supported by transcript-or protein-level evidence.…”

Section: Introductionmentioning

confidence: 99%

Identification of Novel Genes and Proteoforms in Angiostrongylus costaricensis through a Proteogenomic Approach

et al. 2022

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RNA sequencing (RNA-Seq) and mass-spectrometry-based proteomics data are often integrated in proteogenomic studies to assist in the prediction of eukaryote genome features, such as genes, splicing, single-nucleotide (SNVs), and single-amino-acid variants (SAAVs). Most genomes of parasite nematodes are draft versions that lack transcript- and protein-level information and whose gene annotations rely only on computational predictions. Angiostrongylus costaricensis is a roundworm species that causes an intestinal inflammatory disease, known as abdominal angiostrongyliasis (AA). Currently, there is no drug available that acts directly on this parasite, mostly due to the sparse understanding of its molecular characteristics. The available genome of A. costaricensis, specific to the Costa Rica strain, is a draft version that is not supported by transcript- or protein-level evidence. This study used RNA-Seq and MS/MS data to perform an in-depth annotation of the A. costaricensis genome. Our prediction improved the reference annotation with (a) novel coding and non-coding genes; (b) pieces of evidence of alternative splicing generating new proteoforms; and (c) a list of SNVs between the Brazilian (Crissiumal) and the Costa Rica strain. To the best of our knowledge, this is the first time that a multi-omics approach has been used to improve the genome annotation of A. costaricensis. We hope this improved genome annotation can assist in the future development of drugs, kits, and vaccines to treat, diagnose, and prevent AA caused by either the Brazil strain (Crissiumal) or the Costa Rica strain.

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Abdominal angiostrongyliasis: pathologic findings in Swiss mice infected with different doses of Angiostrongylus costaricensis

et al. 2020

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Abstract Abdominal angiostrongyliasis is caused by Angiostrongylus costaricensis, the definitive and intermediate hosts of which are wild rodents and terrestrial molluscs, respectively. Humans are accidental hosts and can be infected by ingesting the third-stage (infective) larvae (L3). It remains unclear whether the number of L3 inoculated is related to lesion severity. Our aim was to analyse histopathological alterations in Swiss mice infected with different doses of A. costaricensis. Thirty-two mice were randomly divided into four groups (n = 8/group): uninfected, control mice; mice infected with a low dose (five L3); mice infected with an intermediate dose (15 L3); and mice infected with a high dose (30 L3). The frequency of intestinal thrombi, splenitis, eggs/larvae, hepatic infarction and acute pancreatitis differed among the groups, the last being considered a significant finding. We conclude that different infective doses alter the histopathological aspects of the infection in Swiss mice, those aspects being more pronounced at medium and high doses, with no effect on the development of the disease. This experimental model shows that the parasite life cycle can be maintained in Swiss mice through the inoculation of a low dose (five L3).

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High-dose enoxaparin in the treatment of abdominal angiostrongyliasis in Swiss mice

Cited by 5 publications

References 11 publications

Relationship between histopathological lesions and oxidative stress in mice infected with Angiostrongylus costaricensis

Relationship between histopathological lesions and oxidative stress in mice infected with Angiostrongylus costaricensis

Identification of Novel Genes and Proteoforms in Angiostrongylus costaricensis through a Proteogenomic Approach

Abdominal angiostrongyliasis: pathologic findings in Swiss mice infected with different doses of Angiostrongylus costaricensis

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