High doses of sodium ascorbate interfere with the expansion of glioblastoma multiforme cells in vitro and in vivo

Ryszawy, Damian; Pudełek, Maciej; Catapano, Jessica; Ciarach, Małgorzata; Setkowicz, Zuzanna; Konduracka, Ewa; Madeja, Zbigniew; Czyż, Jarosław

doi:10.1016/j.lfs.2019.116657

Cited by 15 publications

(24 citation statements)

References 31 publications

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“…Studies showed that specific protein transcription factors significantly decreased, and iron metabolism disrupted after AA treatment in colon cancer cells that are involved in cancer progression (32,33). In Ryszawy et al (34) study, sodium ascorbate showed anti-tumor activity in glioblastoma multiforme cells. Gao et al (35) demonstrated that AA could lead to liver cancer cells' death via intracellular reductive stress.…”

Section: Discussionmentioning

confidence: 96%

Antioxidant Enzyme Activities in Ascorbic Acid and Selenium Applied Hepatocellular Carcinoma Cells

Balkan

Meral

Kısmalı

et al. 2020

Journal of the Turkish Chemical Society Section A: Chemistry

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Ascorbic acid and selenium have potential use in the prevention of cancer. The role of antioxidant enzymes against cancer is to prevent oxidative damage. In this study, the effects of L-selenomethionine (234 µM), SeO2 (100 µM), ascorbic acid (0,313 mM, and 31,3 mM) on antioxidant enzyme activities in HepG2 cell line were studied. GPx, CAT, and SOD activities of the treated cells were measured spectrophotometrically. There was a significant increase in the activity of GPx in HepG2 cells treated with L-selenomethionine (234 µM), SeO2 (100 µM), and ascorbic acid (0,313 Mm and 31,3 mM). However, the activity of CAT was found significantly decreased in L-selenomethionine (234 µM), SeO2 (100 µM), ascorbic acid (31,3 mM) treated HepG2 cells. The SOD levels of HepG2 cells treated with ascorbic acid (0,313 mM and 31,3 mM) were found higher than the control cells. The results of the present study indicated that the antioxidant enzyme activities changed depending on L-selenomethionine, SeO2 application, and CAT activities changed depending on ascorbic acid doses.

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Section: Discussionmentioning

confidence: 96%

Antioxidant Enzyme Activities in Ascorbic Acid and Selenium Applied Hepatocellular Carcinoma Cells

Balkan

Meral

Kısmalı

et al. 2020

Journal of the Turkish Chemical Society Section A: Chemistry

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“…After 96 h, the cells were harvested and counted in the Z2 particle counter (Beckman-Coulter, Indianapolis, IN, USA) to calculate proliferation kinetics. For the estimation of pro-apoptotic potential of the compounds, the cells were seeded at the density of 2 × 10 5 /cm 2 , and treated as described above for 48 h. Subsequently, AnnexinV/Propidium iodide assay was performed (FITC AnnexinV Apoptosis Detection Kit, BD Pharminogen™, San Diego, CA, USA) using ImageStreamX ® cytometer (Amnis Corp, Seattle, WA, USA) as described by Ryszawy et al [42]. Data were analyzed with dedicated IDEAS ® 6.2 software (Amnis Corp, Seattle, WA, USA).…”

Section: Proliferation and Apoptosis Assaysmentioning

confidence: 99%

“…Monomeric derivatives were designed to test their capability to interact or possibly to cross membranes of the model glioblastoma cells. Even though dendron 13 with Pro-terminated arms is neutral, AMP's with four prolines have the ability to cross the blood-brain barrier (BBB) [41,42]. The positively charged and amphiphilic dendrons 14 and 15 contained either four histidine or two nitro-arginine residues, that might provide a cell penetrating function.…”

Section: Synthesis Of Bola Dendrimers 9a and 10amentioning

confidence: 99%

Bioinspired Bola-Type Peptide Dendrimers Inhibit Proliferation and Invasiveness of Glioblastoma Cells in a Manner Dependent on Their Structure and Amphipathic Properties

et al. 2020

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(1) Background: Natural peptides supporting the innate immune system studied at the functional and mechanistic level are a rich source of innovative compounds for application in human therapy. Increasing evidence indicates that apart from antimicrobial activity, some of them exhibit selective cytotoxicity towards tumor cells. Their cationic, amphipathic structure enables interactions with the negatively-charged membranes of microbial or malignant cells. It can be modeled in 3D by application of dendrimer chemistry. (2) Methods: Here we presented design principles, synthesis and bioactivity of branched peptides constructed from ornithine (Orn) assembled as proline (Pro)- or histidine (His)-rich dendrons and dendrimers of the bola structure. The impact of the structure and amphipathic properties of dendrons/dendrimers on two glioblastoma cell lines U87 and T98G was studied with the application of proliferation, apoptosis and cell migration assays. Cell morphology/cytoskeleton architecture was visualized by immunofluorescence microscopy. (3) Results: Dimerization of dendrons into bola dendrimers enhanced their bioactivity. Pro- and His-functionalized bola dendrimers displayed cytostatic activity, even though differences in the responsiveness of U87 and T98G cells to these compounds indicate that their bioactivity depends not only on multiple positive charge and amphipathic structure but also on cellular phenotype. (4) Conclusion: Ornithine dendrons/dendrimers represent a group of promising anti-tumor agents and the potential tools to study interrelations between drug bioactivity, its chemical properties and tumor cells’ phenotype.

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“…Albumin is an indicator for malnutrition which has significantly increased in the intervention group at the end of the study. Fumeron and et al, aimed to evaluate the effect of vitamin C supplement on inflammatory factors and oxidative stress in hemodialysis patients and have suggested that the serum albumin level has increased in the patients under hemodialysis but was not significant compared to the control group 18,19,28 . Quan Jun Yang and et al, have suggested that cancer cachexia is characterized by weight loss, adipose tissue wasting, muscle atrophy and loss of appetite.…”

Section: Resultsmentioning

confidence: 99%

“…Oxidative stress prevention and increasing in antioxidant capacity will affect various health-related and healing processes in the body, including cell aging, inflammation reduction, and cancer-related mechanisms, so human body physiologic mechanisms seem to be a key component in diseases overcoming 18,19,20,21 . Studies have suggested that oral intake of vitamin C can reduce CRP and IL-6 18,22 and even reinforce the immune system.…”

Section: Introductionmentioning

confidence: 99%

Untitled

2020

IJPSR

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Background: Leukemia is the seventh common cancer in Iran, and AML is considered as the most common type. Malnutrition is common among patients with cancer. Vitamin C deficiency has a high prevalence among patients with cancer, which may influence a patient's survival chance. This study was aimed to evaluate the effect of vitamin C supplementation on nutritional status and serum albumin in patients with acute myeloid leukemia referred to Shariati Hospital in Tehran. Methods: This study was recruited by a randomized selection of 50 patients with AML, including entrance criteria, into 2 groups including the supplement group and control group. The duration of the study was 1 month. Nutritional status was assessed by the PG-SGA questionnaire, body weight, serum albumin status, and 3 days of food record before and at the end of the study. Results: The results of this study have shown that the nutritional status and body weight in the supplementation group have improved significantly (p-value=0.001 and 0.004, respectively). The serum albumin status has increased significantly in the supplementation group compared to the control group. (P-value=0.002). Conclusion: The results of this study have suggested that vitamin C supplementation in patients with AML under chemotherapy has improved nutritional status and reduced malnutrition due to chemotherapy side effects.

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High doses of sodium ascorbate interfere with the expansion of glioblastoma multiforme cells in vitro and in vivo

Cited by 15 publications

References 31 publications

Antioxidant Enzyme Activities in Ascorbic Acid and Selenium Applied Hepatocellular Carcinoma Cells

Antioxidant Enzyme Activities in Ascorbic Acid and Selenium Applied Hepatocellular Carcinoma Cells

Bioinspired Bola-Type Peptide Dendrimers Inhibit Proliferation and Invasiveness of Glioblastoma Cells in a Manner Dependent on Their Structure and Amphipathic Properties

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