High-dynamic-range microscope imaging based on exposure bracketing in full-field optical coherence tomography

Leong-Hoï, Audrey; Montgomery, P.C.; Serio, Bruno; Twardowski, Patrice; Uhring, Wilfried

doi:10.1364/ol.41.001313

Cited by 13 publications

(7 citation statements)

References 7 publications

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“… 4 Then the dynamic range can be extended using the high dynamic range (HDR) method, taking several images with exposure bracketing to capture more of the different intensity levels as compared with a single shot. 14 , 4 The important aspect to note with the latter technique is to provide a real increase in the dynamic range and not simply to improve the contrast of a fixed depth image as found in modern digital cameras. While the technique helps in measurements of a wider range of reflectance, on the downside, it is more sensitive to mechanical vibration, the measurement time increasing proportionally with the extra number of bracketing images taken.…”

Section: Tomographic System For Acquiring Depth Imagesmentioning

confidence: 99%

Characterization of Functional Materials Using Coherence Scanning Interferometry and Environmental Chambers

et al. 2023

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Functional materials are challenging to characterize because of the presence of small structures and inhomogeneous materials. If interference microscopy was initially developed for use for the optical profilometry of homogeneous, static surfaces, it has since been considerably improved in its capacity to measure a greater variety of samples and parameters. This review presents our own contributions to extending the usefulness of interference microscopy. For example, 4D microscopy allows real-time topographic measurement of moving or changing surfaces. High-resolution tomography can be used to characterize transparent layers; local spectroscopy allows the measurement of local optical properties; and glass microspheres improve the lateral resolution of measurements. Environmental chambers have been particularly useful in three specific applications. The first one controls the pressure, temperature, and humidity for measuring the mechanical properties of ultrathin polymer films; the second controls automatically the deposition of microdroplets for measuring the drying properties of polymers; and the third one employs an immersion system for studying changes in colloidal layers immersed in water in the presence of pollutants. The results of each system and technique demonstrate that interference microscopy can be used for more fully characterizing the small structures and inhomogeneous materials typically found in functional materials.

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Section: Tomographic System For Acquiring Depth Imagesmentioning

confidence: 99%

Characterization of Functional Materials Using Coherence Scanning Interferometry and Environmental Chambers

et al. 2023

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“…and are initialized as empty set. Gammacorrected exposure time ratios 1 2 , 2 3 and 3 1 cannot be corrupted values since they are computed from pre-determined camera parameters (i.e., exposure time and gamma value) as shown in (2). However, pixel intensity values 1 , 2 and 3 can be corrupted by various types of noises.…”

Section: A Selection Of Noisy Pixelsmentioning

confidence: 99%

Noise Reduction on Bracketed Images for High Dynamic Range Imaging

Shim¹

2020

IJACSA

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The quality of a high dynamic range (HDR) image produced from bracketed images taken at different camera exposure times can be degraded by noise contained in bracketed images. In this paper, we propose a noise reduction method on bracketed images based on exposure time ratio. First, for each pixel pair of a same scene point lying on two different images, the ratio of their intensity values is compared with the ratio of exposure times of the images on which the pixels are lying. If the compared ratios are close, these two pixels are included in noisefree pixels set. The complement of noise-free pixels set is defined as noisy pixels set. Then, the intensity value of each pixel in noisy pixels set is corrected by its expected value computed from noisefree pixel of the same scene point lying on another image. Experimental results show that all the noisy intensity values can be correctly restored from noise-free pixels except the saturated pixels. Denoising results by PSNR show that the proposed method outperforms the other recent denoising methods such as based-on pixel density filter (BPDF), noise adaptive fuzzy switching median filter (NAFSMF), and adaptive Riesz mean filter (ARmF).

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“…High dynamic range (HDR) X-ray radiographic imaging experiments have been shown to accurately resolve the internal features of complicated structural components at different thicknesses [9]. High dynamic range full-field optical coherence tomography based on exposure bracketing has been proposed, demonstrating reduction in the CCD frames spatial noise for improvement in fringe contrast [10]. High dynamic range three dimensional laminar optical tomography has also been demonstrated for increased imaging penetration depth and range in fluorophore detection [11].…”

Section: Introductionmentioning

confidence: 99%

High Dynamic Range Fluorescence Imaging

Vinegoni

Feruglio

Weissleder

2019

IEEE J. Select. Topics Quantum Electron.

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Fluorescence acquisition and image display over a high dynamic range is highly desirable. However, the limited dynamic range of current photodetectors and imaging CCDs impose a limit on the fluorescence intensities that can be simultaneously captured during a single image acquisition. This is particularly troublesome when imaging biological samples, where protein expression fluctuates considerably. As a result, biological images will often contain regions with signal that is either saturated or hidden within background noise, causing information loss. In this manuscript we summarize recent work from our group and others, to extended conventional to high dynamic range fluorescence imaging. These strategies have many biological applications, such as mapping of neural connections, vascular imaging, bio-distribution studies or pharmacologic imaging at the single cell and organ level.

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High-dynamic-range microscope imaging based on exposure bracketing in full-field optical coherence tomography

Cited by 13 publications

References 7 publications

Characterization of Functional Materials Using Coherence Scanning Interferometry and Environmental Chambers

Characterization of Functional Materials Using Coherence Scanning Interferometry and Environmental Chambers

Noise Reduction on Bracketed Images for High Dynamic Range Imaging

High Dynamic Range Fluorescence Imaging

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