High efficiency Hall effect micro-biosensor platform for detection of magnetically labeled biomolecules

Sandhu, Adarsh; Kumagai, Yoshimichi; Łapicki, Adam; Sakamoto, Satoshi; Abe, Masanori; Handa, Hiroshi

doi:10.1016/j.bios.2006.09.021

Cited by 38 publications

(25 citation statements)

References 14 publications

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“…Due to their ability to be easily manipulated by magnetic field gradients and the negligible magnetic background of biological objects, superparamagnetic particles have been increasingly used as labels in volumetric assays (Borlido et al, 2013;Issadore et al, 2014). In biosensing platforms based on the detection of the presence or absence of magnetic particle labeled targets, the read-out is performed in various magnetic stray field detection devices, measuring changes in either static or dynamic properties of the magnetic particles upon interaction with the target, such as magnetoresistance sensors (Baselt et al, 1998;Graham et al, 2003;Dalslet et al, 2011), Hall effect devices (Mihajlovic et al, 2005;Sandhu et al, 2007;Østerberg et al, 2014), magnetic tunnel junctions (Grancharov et al, 2005), superconducting quantum interference devices (Chemla et al, 2000;Grossman et al, 2004;Strömberg et al, 2008), or AC susceptometers (Astalan et al, 2004;Park et al, 2011;Zardán Gómez de la Torre et al, 2011). On the one hand, by employing enzymatic amplification strategies for improving the sensitivity, those magnetic biosensors are capable of detecting nucleic acid targets with a limit of detection (LOD) in the low pM range (Dalslet et al, 2011;Østerberg et al, 2014;Strömberg et al, 2008; Torre et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Blu-ray optomagnetic measurement based competitive immunoassay for Salmonella detection

Tian

Bejhed

Svedlindh

et al. 2016

Biosensors and Bioelectronics

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A turn-on competitive immunoassay using a low-cost Blu-ray optomagnetic setup and two differently sized magnetic particles (micron-sized particles acting as capture particles and nano-sized particles acting as detection particles) is here presented. For Salmonella detection, a limit of detection of 8×10(4)CFU/mL is achieved within a total assay time of 3h. The combination of a competitive strategy and an optomagnetic setup not only enables a turn-on read-out format, but also results in a sensitivity limit about a factor of 20 times lower than of volumetric magnetic stray field detection device based immunoassays. The improvement of sensitivity is enabled by the formation of immuno-magnetic aggregates providing steric hindrance protecting the interior binding sites from interaction with the magnetic nanoparticle labels. The formation of immuno-magnetic aggregates is confirmed by fluorescence microscopy. The system exhibits no visible cross-reaction with other common pathogenic bacteria, even at concentrations as high as 10(7)CFU/mL. Furthermore, we present results when using the setup for a qualitative and homogeneous biplex immunoassay of Escherichia coli and Salmonella typhimurium.

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Section: Introductionmentioning

confidence: 99%

Blu-ray optomagnetic measurement based competitive immunoassay for Salmonella detection

Tian

Bejhed

Svedlindh

et al. 2016

Biosensors and Bioelectronics

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“…So far, magnetic detection of micron or sub-micron sized beads with excellent sensitivity has been demonstrated with both types of sensors, and prediction of detection of much smaller magnetic particles has been made. Until now, however, the magnetic beads were either physically placed on a device’s active region utilizing a micromanipulator [11, 12] or assembled via non-selective blanket self-assembly [10]. The non-selective assembly presents a significant drawback if these devices are to be used in practical multiple target sensing applications.…”

Section: Introductionmentioning

confidence: 99%

The detection of specific biomolecular interactions with micro-Hall magnetic sensors

et al. 2009

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Detection of reagent-free specific biomolecular interactions through sensing of nanoscopic magnetic labels provides one of the promising routes to biosensing with solid-state devices. In particular, Hall sensors based on semiconductor heterostructures have shown exceptional magnetic moment sensitivity over a large dynamic field range suitable for magnetic biosensing using superparamagnetic labels. Here we demonstrate the capability of such micro-Hall sensors to detect specific molecular binding using biotin-streptavidin as a model system. We apply dip-pen nanolithography to selectively biotinylate the active areas of InAs micro-Hall devices with nanoscale precision. Specific binding of complementarily functionalized streptavidin-coated superparamagnetic beads to the Hall crosses occur via molecular recognition, and magnetic detection of the assembled beads is achieved at room temperature using phase-sensitive micro-Hall magnetometry. The experiment constitutes the first unambiguous demonstration of magnetic detection of specific biomolecular interactions with semiconductor micro-Hall sensors, and the selective molecular functionalization and resulting localized bead assembly demonstrate the possibility of multiplexed sensing of multiple target molecules using a single device with an array of micro-Hall sensors.

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“…5. The spherical particles are to be used for carriers for bio-molecular recognition using Hall sensors [8].…”

Section: Monodisperse Ferrite Nano-spheresmentioning

confidence: 99%

Preparation and medical application of magnetic beads conjugated with bioactive molecules

Abe

Nishio

Hatakeyama

et al. 2009

Journal of Magnetism and Magnetic Materials

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High efficiency Hall effect micro-biosensor platform for detection of magnetically labeled biomolecules

Cited by 38 publications

References 14 publications

Blu-ray optomagnetic measurement based competitive immunoassay for Salmonella detection

Blu-ray optomagnetic measurement based competitive immunoassay for Salmonella detection

The detection of specific biomolecular interactions with micro-Hall magnetic sensors

Preparation and medical application of magnetic beads conjugated with bioactive molecules

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