High-Efficiency Intracellular Magnetic Labeling with Novel Superparamagnetic-Tat Peptide Conjugates

Josephson, Lee; Tung, Ching–Hsuan; Moore, and Anna; Weissleder, Ralph

doi:10.1021/bc980125h

Cited by 870 publications

(721 citation statements)

References 26 publications

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“…Thus, the imaging capability provided is not from the SPIO intrinsically, but through their influence on longitudinal and transverse relaxation of the surrounding nuclei. Although the ability of SPIO to significantly reduce the spin-spin relaxation (T2) time is generally relied on for generating MR contrast, 68,99 it has also been demonstrated that SPIO can generate sufficient T1 contrast for biomedical applications as well; SPIO possess both high R1 and R2 relaxivities. 118,127 Upon removal of the magnetic field, Brownian motion is sufficient to randomize the SPIO orientations leaving no magnetic remanence.…”

Section: Magnetismmentioning

confidence: 99%

“…6 Of particular interest is the conjugation of membrane translocating signals such as HIV-Tat and polyArginine peptides to SPIO, which greatly enhance SPIO uptake into many cell types. 68,80 The potential of in vivo cell tracking has been demonstrated in several systems including SPIO loaded T cells. 31 Objects as diminutive as single cells were able to be monitored since the T2 relaxation effect of the sequestered magnetic nanoparticles is exerted over a region larger than that of the cell itself.…”

Section: Cell Trackingmentioning

confidence: 99%

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Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

et al. 2006

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Abstract-The field of molecular imaging has recently seen rapid advances in the development of novel contrast agents and the implementation of insightful approaches to monitor biological processes non-invasively. In particular, superparamagnetic iron oxide nanoparticles (SPIO) have demonstrated their utility as an important tool for enhancing magnetic resonance contrast, allowing researchers to monitor not only anatomical changes, but physiological and molecular changes as well. Applications have ranged from detecting inflammatory diseases via the accumulation of non-targeted SPIO in infiltrating macrophages to the specific identification of cell surface markers expressed on tumors. In this article, we attempt to illustrate the broad utility of SPIO in molecular imaging, including some of the recent developments, such as the transformation of SPIO into an activatable probe termed the magnetic relaxation switch.

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Section: Magnetismmentioning

confidence: 99%

Section: Cell Trackingmentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

et al. 2006

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“…Recently, nanoparticlebased PET agents have been developed for multimodal imaging, however, only a few combined PET/MRI probes have been reported to date, while the majority of the bimodal probes have been developed for use in optical imaging in combination with either PET or MRI. [1], [20], [21], [22], [23] and [24]. Chen and coworkers reported nanoparticle-based probe for dual-modality PET/MRI, by using MnMEIO as MRI agent and 124 I for PET [25].…”

Section: Introductionmentioning

confidence: 99%

The cell labeling efficacy, cytotoxicity and relaxivity of copper-activated MRI/PET imaging contrast agents

et al. 2011

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/npsi/ctrl?action=rtdoc&an=19739632&lang=en http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/ctrl?action=rtdoc&an=19739632&lang=fr READ THESE TERMS AND CONDITIONS CAREFULLY BEFORE USING THIS WEBSITE.http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/jsp/nparc_cp.jsp?lang=en Vous avez des questions? Nous pouvons vous aider. Pour communiquer directement avec un auteur, consultez la première page de la revue dans laquelle son article a été publié afin de trouver ses coordonnées. Si vous n'arrivez pas à les repérer, communiquez avec nous à PublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. Questions?Contact the NRC Publications Archive team at PublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. If you wish to email the authors directly, please see the first page of the publication for their contact information. NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. For the publisher's version, please access the DOI link below./ Pour consulter la version de l'éditeur, utilisez le lien DOI ci-dessous.http://doi.org/10.1016/j.biomaterials.2010.10.013 Biomaterials, 32, 4, pp. 1167Biomaterials, 32, 4, pp. -1176Biomaterials, 32, 4, pp. , 2010 The cell labeling efficacy, cytotoxicity and relaxivity of copperactivated MRI/PET imaging contrast agents AbstractA new class of nanoparticle-based dual-modality positron emission tomography/magnetic resonance imaging (PET/MRI) contrast agents has been developed. The probe consists of a superparamagnetic iron oxide (SPIO) or manganese oxide core coated with 3,4-dihydroxy-d,l-phenylalanine (dl-DOPA). The chelator 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) was conjugated to DOPA termini. The DOTA modified nanoparticles allow chelation of copper for PET imaging. These surface functionalized nanoparticle-based probes have been characterized by various analytical techniques. The cell-labeling efficacy, cytotoxicity and relaxivity of these nanoparticles have been evaluated and compared with the same properties of one of the most commonly utilized MRI contrast agents, Feridex ® . Evidently, this new nanoparticle has a great potential for use in cell tracking with MRI and PET in the absence of transfecting agent. It is noteworthy that there is a sharp increase in r 2 relaxivity of these nanoparticles on coordination with Cu 2+ ions. Thus these iron oxide nanoparticles can also be explored as the smart magnetic resonance (MR) sensor for the detection of micromolar changes in copper concentration for neurodegenerative diseases such as Alzheimer's disease, Menkes and Wilson's diseases, amyotrophic lateral sclerosis and prion diseases.

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“…It is known to act as a 'vector' to direct the protein into the cell nucleus through the nuclear pore complex, [17,18] and was recently applied to Au-and dextran-coated iron oxide nanoparticles for their targeting to cell nuclei. [19][20][21][22] Different from these previous functionalization steps, our approach is to conjugate biotinylated NLS to monodisperse Fe 3 O 4 nanoparticles through NeutrAvidin (NAv) and a surfactant combination of polyethylene glycol (PEG) and dopamine (DPA), or 4-(2-aminoethyl)benzene-1,2-diol. DPA can form a strong chelate chemical bond with the iron oxide surface, [23,24] and PEG has been used widely to protect nanoparticles for their stabilization under physiological conditions.…”

Section: Introductionmentioning

confidence: 99%

Monodisperse Magnetite Nanoparticles Coupled with Nuclear Localization Signal Peptide for Cell‐Nucleus Targeting

Xie

Kohler

et al. 2008

Chemistry An Asian Journal

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Functionalization of monodisperse superparamagnetic magnetite (Fe 3 O 4 ) nanoparticles for cell specific targeting is crucial for cancer diagnostics and therapeutics. Targeted magnetic nanoparticles can be used to enhance the tissue contrast in magnetic resonance imaging (MRI), to improve the efficiency in anticancer drug delivery, and to eliminate tumor cells by magnetic fluid hyperthermia. Herein we report the nucleus-targeting Fe 3 O 4 nanoparticles functionalized with protein and nuclear localization signal (NLS) peptide. These NLS-coated nanoparticles were introduced into the HeLa cell cytoplasm and nucleus, where the particles were monodispersed and non-aggregated. The success of labeling was examined and identified by fluorescence microscopy and MRI. The work demonstrates that monodisperse magnetic nanoparticles can be readily functionalized and stabilized for potential diagnostic and therapeutic applications.

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High-Efficiency Intracellular Magnetic Labeling with Novel Superparamagnetic-Tat Peptide Conjugates

Cited by 870 publications

References 26 publications

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

The cell labeling efficacy, cytotoxicity and relaxivity of copper-activated MRI/PET imaging contrast agents

Monodisperse Magnetite Nanoparticles Coupled with Nuclear Localization Signal Peptide for Cell‐Nucleus Targeting

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