High-efficiency, microprojectile-mediated cotransformation of sugarcane, using visible or selectable markers

Bower, Robert; Elliott, Adrian R.; Potier, Bernard; Birch, Robert G.

doi:10.1007/bf00564201

Cited by 137 publications

(126 citation statements)

References 25 publications

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“…These properties make albD an attractive candidate for transfer into sugarcane (39) to confer albicidin resistance and to determine the role of albicidin phytotoxins in systemic disease development.…”

Section: Discussionmentioning

confidence: 99%

The gene for albicidin detoxification from Pantoea dispersa encodes an esterase and attenuates pathogenicity of Xanthomonas albilineans to sugarcane

Zhang

Birch²

1997

Proc. Natl. Acad. Sci. U.S.A.

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Albicidin phytotoxins are pathogenicity factors in a devastating disease of sugarcane known as leaf scald, caused by Xanthomonas albilineans. A gene (albD) from Pantoea dispersa has been cloned and sequenced and been shown to code for a peptide of 235 amino acids that detoxifies albicidin. The gene shows no significant homology at the DNA or protein level to any known sequence, but the gene product contains a GxSxG motif that is conserved in serine hydrolases. The AlbD protein, purified to homogeneity by means of a glutathione S-transferase gene fusion system, showed strong esterase activity on p-nitrophenyl butyrate and released hydrophilic products during detoxification of albicidins. AlbD hydrolysis of p-nitrophenyl butyrate and detoxification of albicidins required no complex cofactors. Both processes were strongly inhibited by phenylmethylsulfonyl f luoride, a serine enzyme inhibitor. These data strongly suggest that AlbD is an albicidin hydrolase. The enzyme detoxifies albicidins efficiently over a pH range from 5.8 to 8.0, with a broad temperature optimum from 15 to 35°C. Expression of albD in transformed X. albilineans strains abolished the capacity to release albicidin toxins and to incite disease symptoms in sugarcane. The gene is a promising candidate for transfer into sugarcane to confer a form of disease resistance.

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Section: Discussionmentioning

confidence: 99%

The gene for albicidin detoxification from Pantoea dispersa encodes an esterase and attenuates pathogenicity of Xanthomonas albilineans to sugarcane

Zhang

Birch²

1997

Proc. Natl. Acad. Sci. U.S.A.

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“…In order to control aphids it will be an accessible alternative pathway. Nowadays suitable protocols for sugarcane direct transformation by particle bombardment are available (Bower et al 1996;Zhang et al 2000). Direct transformation procedures, such as particle bombardment and electroporation, have several disadvantages because of poor reproducibility, variable transgene copy number, and the instability of the gene construct in the new host.…”

Section: Introductionmentioning

confidence: 99%

Improved Agrobacterium-mediated genetic transformation of GNA transgenic sugarcane

et al. 2007

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Six plasmids carrying a snowdrop lectin (Galanthus nivalis agglutinin, GNA) and one of three selection markers were successfully transferred into two sugarcane cultivars (FN81-745 and Badila) via Agrobacterium-mediated transformation. Agrobacterium strains LBA4404, EHA105 and A281 that harboured a super-binary vector were used for sugarcane transformation. The use of the hygromycin (Hyg) resistance gene (hpt II), phosphinothrincin (PPT) resistance gene (bar) or G418 resistance gene (npt II) as a screenable marker facilitated the initial selection of GNA transgenic sugarcane callus with different efficiencies and helped the rapid segregation of individual transformation events. All the three selective marker genes were controlled by CaMV 35S promoter, while GNA gene was controlled by promoter of RSs-1 (rice sucrose synthase-1) or Ubi (maize ubiquitin). Factors important to successful transformation mediated by Agrobacterium tumefaciens were optimized, which included concentration of A. tumefaciens, medium composition, co-cultivated methods with plant tissue, strain virulence and different selective marker genes. An efficient protocol for sugarcane transformation mediated by A. tumefaciens was established. The GNA gene has been integrated into sugarcane genome as demonstrated by PCR and Southern dot blotting detections. The preliminary results from bioassay demonstrated a significant resistance of the transgenic sugarcane plants to woolly aphid (Ceratovacuna lanigera Zehnther) indicating thus the possibility for obtaining a transgenic sugarcane cultivar with resistance to woolly aphid.

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“…Callus cultures establishment and maintenance is a labor demanding and the regenerated plants are ready for green house planting in at least 36 weeks (Bower et al, 1996). From cell, tissue and organ cultures, production of somatic embryo-like structures may happen either directly or indirectly (Reinert et al, 1977 andWarren andFowler, 1977).…”

Section: Plant Tissue Culture Studiesmentioning

confidence: 99%

An Overview of Biotechnological Approaches for Crop Plant Improvement

Ijaz

Rana²

2012

gab

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Conventional crop breeding is being restricted due to the use of only closely related species and it takes many generations to initiate significant improvement. Biotechnology is getting a great interest as basic techniques for tailoring crop plants as per specific requirement. It allows researchers to shorten this time and this imitate the evolutionary process but in highly selective way. As a biotechnological approach, genetic manipulation strategy is something like tearing out a page of the instruction manual for one organism and gluing it into the instruction manual of another organism. Around the globe, this emerging science is playing an imperative role to help agricultural productivity. In this review, biotechnological approaches that are commonly used for the improvement of crop plants are highlighted and briefly described.

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High-efficiency, microprojectile-mediated cotransformation of sugarcane, using visible or selectable markers

Cited by 137 publications

References 25 publications

The gene for albicidin detoxification from Pantoea dispersa encodes an esterase and attenuates pathogenicity of Xanthomonas albilineans to sugarcane

The gene for albicidin detoxification from Pantoea dispersa encodes an esterase and attenuates pathogenicity of Xanthomonas albilineans to sugarcane

Improved Agrobacterium-mediated genetic transformation of GNA transgenic sugarcane

An Overview of Biotechnological Approaches for Crop Plant Improvement

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