High efficiency of genetic transformation and regeneration of Artemisia annua L. via Agrobacterium tumefaciens-mediated procedure

An improved Agrobacterium tumefaciens mediated transformation of Artemisia annua L. by using stem internodes as explants

“…The optimal concentration was at 100 µmol/l acetosyringone, resulting in 45 kanamycin resistant shoots per 100 explants, compared to 17 transformed shoots per 100 explants when no acetosyringone was used. These results contradict a previous report where acetosyringone did not significantly enhance the transformation efficiency of A. annua (Han et al 2005) and it may be due to the fact that different types of explants were used there.…”

“…The penicillin derived antibiotics for decontamination were shown to be more effective, but limited regeneration of shoots from transgenic calli has been observed (Vergauwe et al 1996b). Han et al (2005) used cefotaxime as decontaminating antibiotic, but it reduced callus formation and inhibited shoot induction on leaf explants (Vergauwe et al 1996a).…”

Effect of Antisense Squalene Synthase Gene Expression on the Increase of Artemisinin Content in Artemisia anuua

“…Plant transformation was basically performed according to the method of Han et al (2005). The leaves of 2-week-old aseptic seedlings were immersed in 50 mL A. tumefaciens, which was at the log phase of growth and was diluted 10-fold with MS medium.…”

Ri-gene transformation into leaf tissues of Artemisia annua L. and cultivation of hairy-root for artemisinin accumulation

“…However, some other plant species may respond better to other strains of A. Rhizogenes. Successful gene transfer to callus tissue regeneration shoots [4], suspension [5] and roots [6,7,8] have resulted in increased levels of artemisinin synthesized in the cell 1-5 times.…”