High-efficiency second generation ethanol from the hemicellulosic fraction of softwood chips mixed with construction and demolition residues

Boboescu, Iulian-Zoltan; Gélinas, Malorie; Beigbeder, Jean-Baptiste; Lavoie, Jean‐Michel

doi:10.1016/j.biortech.2018.06.056

Cited by 16 publications

(6 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The organic acids, furan aldehydes and ethanol were quantified using an Agilent 1100 series HPLC (High Performance Liquid Chromatography) equipped with a G1362A Refractive Index Detector (temperature was set to 40°C) and ROA-Organic Acid H+ (8%) analysis column as described previously. 5 Quantification of monomeric carbohydrates was performed using a Dionex ICS-5000 + ion chromatography system equipped with a KOH eluent generator and a Dionex CarboPac SA10-4lM column set at 45°C. 26 The microalgal phytoremediation potential was evaluated by measuring the total organic carbon concentration of the liquid supernatant using a total organic carbon analyzer (Shimadzu TOC-Ve, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…On the other hand, the lignocellulosic ethanol production process represents a complex approach constituting of several subsequent carbohydrate extraction, hydrolysis and in some cases, detoxification steps prior to their fermentation. 5 The lignocellulosic matrix encasing these constitutive carbohydrates requires various invasive physical, chemical, thermal, biological or enzymatic pre-treatment steps prior to their extraction. 6 However, these sometimes severe treatment conditions can lead to the production of undesirable furans, phenolic compounds and various organic acids with inhibitory effects on the subsequent fermentation processes.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transitioning Towards a Circular Economy in Québec: An Integrated Process for First-, Second- and Third-Generation Ethanol from Sweet Sorghum and Chlorella vulgaris Biomass

Boboescu

Beigbeder

Damay

et al. 2019

Industrial Biotechnology

Self Cite

View full text Add to dashboard Cite

Full feedstock potential needs to be tapped to make lignocellulosic ethanol an economically viable reality. This work focuses on the Saccharomyces cerevisiae ethanol fermentation of fresh sorghum carbohydrates extracted through a mild steam-treatment process, and the subsequent Chlorella vulgaris cultivation using the generated liquid and gaseous fermentation effluents. The first section of the manuscript focuses on the effect of nutrient addition (fermentation effluent, yeast extract and urea) on the conversion efficiency of the sorghum carbohydrates to ethanol. Overall, the fermentation time was reduced to half when yeast extract and urea were supplemented to the free and hemicellulosic carbohydrate stream, accelerating the total sugar consumption time from 24 h to under 12 h. However, regarding the cellulosic carbohydrate hydrolysate, the sole addition of urea resulted in a slight improvement of the fermentation kinetics. The second half of the manuscript presents the impact of these different fermentation effluents and various process parameters (addition of yeast extract, antibiotic and CO 2 ) on the microalgal cultivation and composition. The cellulosic hydrolysate yielded the highest concentrations of microalgal carbohydrates (507 mg/L) under a CO 2 -rich environment. Further cultivation scale-up assays confirmed these observations in the presence of 10% CO 2 using the mixed fermentation effluents of the free and constitutive sorghum carbohydrates. Thus, an integrated sorghum-based first-(free carbohydrates), second-(constitutive carbohydrates) and third-generation (microalgal carbohydrates) ethanol production process was thoroughly investigated. This work could represent a step towards bridging the gap leading to full-scale commercialization of these advanced-biofuel technologies.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transitioning Towards a Circular Economy in Québec: An Integrated Process for First-, Second- and Third-Generation Ethanol from Sweet Sorghum and Chlorella vulgaris Biomass

Boboescu

Beigbeder

Damay

et al. 2019

Industrial Biotechnology

Self Cite

View full text Add to dashboard Cite

show abstract

“…[25] In work performed at the University of Sherbrooke, steam pretreatment of sulphuric acid-impregnated woody biomass provided a hemicellulose solution, which was then fermented. [53] Most hydrolysis research in Canada is focused on the fermentation pathway to generate bioethanol. However, a study conducted at the University of Toronto and Aalto University, Finland, F I G U R E 6 Biochemical route for woody biomass valorization looked into a method for manufacturing 4-O-methyl Dglucaric acid (a dicarboxylic acid) from hardwood xylan (glucuronoxylan).…”

Section: Fast Pyrolysismentioning

confidence: 99%

“…Laboratory-and pilotscale-process development Bioethanol TRL 4 Boboescu et al [53] Two-step mechanical extrusion pretreatment ! Fermentation !…”

Section: Of Softwood With Construction and Demolition Residuesmentioning

confidence: 99%

“…With the most relevant nutrient‐ and yeast inoculum‐related factors, Boboescu et al developed a thorough process characterization, statistical optimization, and bioreactor scale‐up of a hemicellulosic ethanol production system. [ 53 ] Their study used a hemicellulosic solution as a fermentation substrate, which was created by pretreating softwood and construction and demolition waste streams with steam explosion and acid hydrolysis. The fermentation technique described in their study used Saccharomyces cerevisiae as the active dry yeast and achieved an ethanol yield of 81.2% (based on the availability of total monomeric carbohydrates).…”

Section: Biochemical Conversionmentioning

confidence: 99%

See 1 more Smart Citation

A review of Canadian wood conversion technologies for the production of fuels and chemicals

et al. 2023

View full text Add to dashboard Cite

Canada has 347 million ha of forest cover, contributing to the potential large availability of wood‐based resources. Although Canada's forest sector contributed $23.7 billion to the national nominal gross domestic product (GDP) in 2019, the GDP contribution of the wood product manufacturing subsector shrank by 6%. To reposition the Canadian forest industry, new forest management practices and wood‐based conversion technologies should be applied. In this context, the use of woody biomass in biorefineries to produce clean energy, fuels, and chemicals is becoming increasingly significant. There is a need to understand the current status and challenges of the wood‐based biomass conversion technologies that have been and are being developed in Canada. This information will help decision‐makers in formulating and implementing forest sector‐related policies for a sustainable bioeconomy in Canada. This study is focused on a review of Canadian woody biomass conversion technologies. Our critical review identified considerable potential biomass conversion technologies specialized for woody feedstock, all in the Canadian setting. We focused on the prospects of revitalizing Canada's pulp and paper industry through the integration of pre‐treatment processes and biochemical technologies. The thermochemical conversion pathway was identified as the dominant route for woody feedstock valorization. The review also identified pathways with the potential to diversify the existing product mix that generate products from wood streams, such as chemicals and biomaterials. Most of the biochemical and thermochemical research done in institutional and multi‐institutional research collaborations from laboratory scale to industrial scale will boost the chances of the commercialization of a wood‐based biorefinery in Canada.

show abstract

Exploiting the NADPH pool for xylitol production using recombinant Saccharomyces cerevisiae

Reshamwala

Lali

2020

Biotechnology Progress

View full text Add to dashboard Cite

Xylitol is a five‐carbon sugar alcohol that has a variety of uses in the food and pharmaceutical industries. In xylose assimilating yeasts, NAD(P)H‐dependent xylose reductase (XR) catalyzes the reduction of xylose to xylitol. In the present study, XR with varying cofactor specificities was overexpressed in Saccharomyces cerevisiae to screen for efficient xylitol production. Xylose consumption and xylitol yields were higher when NADPH‐dependent enzymes (Candida tropicalis XR and S. cerevisiae Gre3p aldose reductase) were expressed, indicating that heterologous enzymes can utilize the intracellular NADPH pool more efficiently than the NADH pool, where they may face competition from native enzymes. This was confirmed by overexpression of a NADH‐preferring C. tropicalis XR mutant, which led to decreased xylose consumption and lower xylitol yield. To increase intracellular NADPH availability for xylitol production, the promoter of the ZWF1 gene, coding for the first enzyme of the NADPH‐generating pentose phosphate pathway, was replaced with the constitutive GPD promoter in a strain expressing C. tropicalis XR. This change led to a ~12% increase in xylitol yield. Deletion of XYL2 and SOR1, whose gene products can use xylitol as substrate, did not further increase xylitol yield. Using wheat stalk hydrolysate as source of xylose, the constructed strain efficiently produced xylitol, demonstrating practical relevance of this approach.

show abstract

High-efficiency second generation ethanol from the hemicellulosic fraction of softwood chips mixed with construction and demolition residues

Cited by 16 publications

References 28 publications

Transitioning Towards a Circular Economy in Québec: An Integrated Process for First-, Second- and Third-Generation Ethanol from Sweet Sorghum and Chlorella vulgaris Biomass

Transitioning Towards a Circular Economy in Québec: An Integrated Process for First-, Second- and Third-Generation Ethanol from Sweet Sorghum and Chlorella vulgaris Biomass

A review of Canadian wood conversion technologies for the production of fuels and chemicals

Exploiting the NADPH pool for xylitol production using recombinant Saccharomyces cerevisiae

Contact Info

Product

Resources

About