High expression levels of cyclin B1 and Polo-like kinase 1 in ectopic endometrial cells associated with abnormal cell cycle regulation of endometriosis

Tang, Li; Wang, Tingting; Wu, Yanting; Zhou, Caiyun; Huang, He‐Feng

doi:10.1016/j.fertnstert.2008.01.041

Cited by 32 publications

(22 citation statements)

References 52 publications

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“…It is consistent with clinical application of large dose progesterone in treatment for endometrial proliferative disorders. As abundant expression of Cyclin B1 and alteration of the G2 pathway was reported in abnormal proliferative endometrial tissues, such as endometrial hyperplasia, endometrial adenocarcinoma and endometriosis[16,18,19,22-24] the down-regulation of cyclin B1 detected under the treatment of progesterone could well explain the underlying mechanism of the inhibitory effect of progesterone on endometrial cell growth.…”

Section: Discussionmentioning

confidence: 99%

Involvement of cyclin B1 in progesterone-mediated cell growth inhibition, G2/M cell cycle arrest, and apoptosis in human endometrial cell

Tang

Pan

Luo

et al. 2009

Reprod Biol Endocrinol

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BackgroundProgesterone plays an important role in the proliferation and differentiation of human endometrial cells (hECs). Large-dose treatment with progesterone has been used for treatment of endometrial proliferative disorders. However, the mechanisms behind remain unknown.MethodsTo investigate the role of cyclin B1 in proliferation and differentiation of hECs in menstrual cycle, the expression of cyclin B1 throughout the menstrual cycle was evaluated in hECs. To determine the effects of progesterone on the proliferation, cell cycle progression and apoptosis of hECs and to test if cyclin B1 is involved in these effects, progesterone and/or Alsterpaullone (Alp, a specific inhibitor of Cyclin B1/Cdc2) were added to primary hECs. Cellular proliferation was evaluated with MTT test, cell cycle with propidium iodide (PI) staining and flow cytometry, apoptosis with FITC-Annexin V and the expression of cyclin B1 with Western blotting.ResultsThe expression level of cyclin B1 in secretory endometria was significantly lower than in proliferative endometria (p < 0.01). Progesterone significantly inhibited the growth of hECs in a concentration-dependent manner (P < 0.01). The treatment with progesterone significantly decreased the expression of cyclin B1, increased the proportions of cell in G2/M phase, and apoptotic cells (P < 0.05 for all). The presence of Alp significantly enhanced the effects of progesterone on cyclin B1 down-regulation, G2/M cell cycle arrest and induction of apoptosis (P < 0.01 for all).ConclusionOur findings suggest that cyclin B1 is a critical factor in proliferation and differentiation of hECs. Progesterone may inhibit cell proliferation, mediate G2/M cell cycle arrest and induce apoptosis in hECs via down-regulating Cyclin B1.

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Section: Discussionmentioning

confidence: 99%

Involvement of cyclin B1 in progesterone-mediated cell growth inhibition, G2/M cell cycle arrest, and apoptosis in human endometrial cell

Tang

Pan

Luo

et al. 2009

Reprod Biol Endocrinol

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“…[5] . As a cell cycle control kinase, polo-like kinase1 (PLK1) and its overexpression are highly associated with many human cancers, including bladder [6] , breast [7][8][9][10] , colorectal [11,12] , endometrial [13,14] , esophageal [15][16][17] , gastric [18][19][20][21] , glioma [22] , hepatoblastoma [23] , hepatocellular carcinoma [24] , head and neck [25] , leukemia and lymphoma [26,27] , melanoma [28,29] , nonsmall-cell lung [30] , ovarian [31] , papillary [32] , pancreatic [33,34] , prostate [35] and thyroid cancer [36] . However, up to now, as far as we know, there are few studies available on the overexpression of PLK in HCC.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of polo-like kinase1 predicts a poor prognosis in hepatocellular carcinoma patients

He²,

Lin³

et al. 2009

WJG

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AIM:To elucidate the role of overexpressed polo-like kinase1 (PLK1) in hepatocellular carcinoma (HCC). METHODS:We prospectively collected clinicopathological, immunohistochemical and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) data from 135 HCC patients undergoing successful hepatectomy. The correlations between PLK1 mRNA expression and clinicopathologic variables were analyzed by Mann-Whitney U test. Prognostic factors were identified by univariate and multivariate analyses. RESULTS:Immunohistochemical results showed overexpression of PLK1 was mainly found in tumor tissues compared with tumor-free tissue. A similar mRNA result was obtained by semi-quantitative RT-PCR. A total of 111 samples were positive for PLK1 mRNA expression. The positive expression was correlated with venous invasion, tumor nodules and Edmondson grade. Furthermore, 1, 3, 5-year survival rates in the positive expression group were significantly lower than the negative control group. Multivariate analysis showed that positive PLK1 expression was an independent risk factor for HCC.

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“…Cyclin B1 binds to cdc2 at the beginning of the G2 phase, forming an activated Cyclin B1-cdc2 complex (maturation-promoting factor) and then phosphorylates a number of important substrates to control the G2 to M transition. Previous study has revealed the (30). Whatever the putative role of IMP1 and Cyclin B1 in endometriosis is, the clinical significance of the higher serum IMP1 and Cyclin B1 autoantibody levels in women with ovarian endometrioma warrants consideration.…”

Section: Discussionmentioning

confidence: 97%

“…Previous study has revealed the Receiver operating characteristic (ROC) curves of MP1 and Cyclin B1 autoantibody for the diagnosis of ovarian endometrioma. (30). Whatever the putative role of IMP1 and Cyclin B1 in endometriosis is, the clinical significance of the higher serum IMP1 and Cyclin B1 autoantibody levels in women with ovarian endometrioma warrants consideration.…”

Section: Figmentioning

confidence: 99%

Evaluation of serum autoantibody levels in the diagnosis of ovarian endometrioma

Yi¹,

Wang²,

Chao³

et al. 2010

Clinical Laboratory Analysis

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High expression levels of cyclin B1 and Polo-like kinase 1 in ectopic endometrial cells associated with abnormal cell cycle regulation of endometriosis

Cited by 32 publications

References 52 publications

Involvement of cyclin B1 in progesterone-mediated cell growth inhibition, G2/M cell cycle arrest, and apoptosis in human endometrial cell

Involvement of cyclin B1 in progesterone-mediated cell growth inhibition, G2/M cell cycle arrest, and apoptosis in human endometrial cell

Overexpression of polo-like kinase1 predicts a poor prognosis in hepatocellular carcinoma patients

Evaluation of serum autoantibody levels in the diagnosis of ovarian endometrioma

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