High expression levels of TLR9 and PD-L1 indicates a poor prognosis in patients with angioimmunoblastic T-cell lymphoma: a retrospective study of 88 cases in a single center

Abstract

Background: The role of TLR9 expressed by tumor cells in evading immune surveillance was confirmed. PD-L1 expression in tumor cells plays a key role in tumor immune escape, which is associated with poor prognosis. However, the clinical relevance of TLR9 and PD-L1 expression in angioimmunoblastic T-cell lymphoma (AITL) has not been evaluated.Materials and methods: In this study, we identified differentially expressed genes in AITL samples by bioinformatic analysis, and we first examined TLR9 and PD-L1 expression by immunohistochemical staining in patients with AITL and compared these data with clinical features and survival time.Results: It was found that the expression of PD-L1 and multiple TLRs was higher in AITL than normal T-cell samples, and TLR9 and PD-L1 expression displayed complex interactions by bioinformatic analysis. The rates of TLR9 and PD-L1 high expression were 69% and 50%, respectively. High expression of either TLR9 or PD-L1 indicated a poor survival rate for patients with AITL. Multivariate analysis further confirmed that high expression levels of TLR9 and PD-L1 were unfavorable prognostic factors for AITL. We further found inferior overall survival in AITL with clinical features of ECOG status ≥ 2, advanced-stage, elevated serum LDH levels, elevated serum β2-MG levels, and high IPI score.Conclusion: TLR9 and PD-L1 expression may be a novel predictor of prognosis for patients with AITL and may serve as potential therapeutic strategy.

CXCL6 fuels the growth and metastases of esophageal squamous cell carcinoma cells both in vitro and in vivo through upregulation of PD‐L1 via activation of STAT3 pathway

“…Surprisingly, we have not observed a significant association of CXCL6 with overall prognosis in ESCC. Much like CXCL6, significant association has also failed to be reached between PD‐L1 expression and overall survival of patients with ESCC in our setting, which was inconsistent with a great deal of studies claiming that overexpressed PD‐L1 was markedly associated with shorter survival in tumors, regardless of their origins (Carlsson et al, 2020; Qian et al, 2020; Suzuki et al, 2020; Wilms et al, 2020). Fortunately, the observation we made concerning the prognostic prediction of PD‐L1 in ESCC was in step with one meta‐analysis performed in oral squamous cell carcinoma by Troiano et al (2019), concluding that PD‐L1 expression was unassociated with unfavorable prognoses.…”

Metabolomics Based on Peripheral Blood Mononuclear Cells to Dissect the Mechanisms of Chaigui Granules for Treating Depression

“…The protein–protein interaction network (PPI network) was obtained by importing the common targets from the Wayne diagram into the STRING () online database for analysis . Moreover, Cytoscape software was used to create PPI networks and identify hub proteins with the Cytohubba plugin .…”

Education and Empowering Special Forces to Eradicate Secret Defectors: Immune System-Based Treatment Approaches for Mature T- and NK-Cell Malignancies

“…Using the ≥50% cutoff, authors find 79% ENKTL, 71% ALK + ALCL, 39% ALK − ALCL, and 36% of PTCL-NOS cases positive for PD-L1. Overall, studies have shown that MTCL/L cells frequently express PD-1/PD-L1 molecules (and that respective expression levels can correlate with prognostic perspectives [117,118]). Interestingly, for ENKTL, PD-1 expression was rarely or not detected (0-2% of cases) while PD-L1 expression could be found in 56-80% of cases.…”