High Expression of CXCL10/CXCR3 in Ventilator-Induced Lung Injury Caused by High Mechanical Power

Xie, Yongpeng; Zheng, Hui; Mou, Zhifang; Wang, Yanli; Li, Xiaomin

doi:10.1155/2022/6803154

Cited by 10 publications

(6 citation statements)

References 30 publications

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“…CXCL10 promoted the occurrence and development of lung injury by binding to CXCR3 [ 23 ]. In addition, in our previous study, it was confirmed that the level of CXCL10 increased significantly in ARDS patients and the enhanced inflammatory response promoted the progression of lung injury [ 24 ]. Therefore, it was suggested that the CXCL10/CXCR3 pathway might play an important role in the mechanism of lung injury during endogenous ARDS.…”

Section: Discussionmentioning

confidence: 66%

Whole Transcriptomic Analysis of Key Genes and Signaling Pathways in Endogenous ARDS

Xie

Luo

Hu³

et al. 2022

Disease Markers

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Objective. To analyze the differentially expressed genes (DEGs) in rats with endogenous acute respiratory distress syndrome (ARDS) lung injury and explore the pathogenesis and early diagnostic molecular markers using whole transcriptomic data. Methods. Twelve 8-week-old male Sprague Dawley rats were selected and randomly and equally divided into ARDS lung injury group and normal control group. RNA was extracted from the left lung tissues of both the groups and sequenced using the paired-end sequencing mode of the Illumina Hiseq sequencing platform. The DEGs of miRNA, cirRNA, lncRNA, and mRNA were screened using DESeq2 software, and the ceRNA regulatory network was constructed using Cytoscape. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed using the mRNA DEGs. STRING and Cytoscape software were used to construct the protein interaction network and identify the 15 key genes, which were verified using quantitative real-time polymerase chain reaction (qRT-PCR). Results. Based on different screening conditions, and compared with the control group, the ARDS lung injury group showed 836 mRNA DEGs (386 upregulated and 450 downregulated), 110 lncRNA DEGs (53 upregulated and 57 downregulated), 19 circRNA DEGs (3 upregulated and 16 downregulated), and 6 miRNA DEGs (5 upregulated and 1 downregulated gene). GO showed that the DEGs of mRNA were mainly involved in biological processes, such as defense response to lipopolysaccharide and other organisms, leukocyte chemotaxis, neutrophil chemotaxis, and cytokine-mediated signaling. KEGG enrichment analysis showed that the DEGs played their biological roles mainly by participating in IL-17, TNF, and chemokine signaling pathways. The PPI analysis showed a total of 281 node proteins and 634 interaction edges. The top 15 key genes, which were screened, included Cxcl10, Mx1, Irf7, Isg15, Ifit3, Ifit2, Rsad2, Ifi47, Oasl, Dhx58, Usp18, Cmpk2, Herc6, Ifit1, and Gbp4. The ceRNA network analysis showed 69 nodes and 73 correlation pairs, where the key gene nodes were miR-21-3p, Camk2g, and Stx2. Conclusions. The chemotaxis, migration, and degranulation of inflammatory cells, cytokine immune response, autophagy, and apoptosis have significant biological functions in the occurrence and development of endogenous acute lung injury during ARDS. Thus, the camk2g/miR-21-3p/lncRNA/circRNA network, CXCL10/CXCR3, and IL-17 signaling pathways might provide novel insights and targets for further studying the lung injury mechanism and clinical treatment.

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Section: Discussionmentioning

confidence: 66%

Whole Transcriptomic Analysis of Key Genes and Signaling Pathways in Endogenous ARDS

Xie

Luo

Hu³

et al. 2022

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“…The MP represents the energy delivered to the respiratory system by mechanical ventilation, which results from a combination of ventilatory variables. Therefore, MP might have a higher predictive value for patient-centered outcomes, as different combinations of its components may yield a similar damage to the lung [ 4 - 8 ]. The ability to combine the effect of time-varying ventilatory variables seems particularly attractive in spontaneously breathing patients.…”

Section: Discussionmentioning

confidence: 99%

“…Lastly, we showed that early PP may reduce MP WAL while improving oxygenation and CO2 clearance during NIV. Therefore, early PP therapy may be a more “energetically” advantageous strategy to relieve hypoxemia than PEEP uptitration, which may overdistend the lung and increase MP and lung injury when it does not recruit atelectatic lung tissue [ 8 , 35 ]. The association of PEEP levels and NIV duration with COVID-19 pneumonia severity and mortality is consistent with this view [ 11 - 13 ].…”

Section: Discussionmentioning

confidence: 99%

Mechanical power normalized to aerated lung predicts noninvasive ventilation failure and death and contributes to the benefits of proning in COVID-19 hypoxemic respiratory failure

Musso,

Taliano,

De Iuliis

et al. 2023

EPMA Journal

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Background Concern exists that noninvasive ventilation (NIV) may promote ventilation-induced lung injury(VILI) and worsen outcome in acute hypoxemic respiratory failure (AHRF). Different individual ventilatory variables have been proposed to predict clinical outcomes, with inconsistent results. Mechanical power (MP), a measure of the energy transfer rate from the ventilator to the respiratory system during mechanical ventilation, might provide solutions for this issue in the framework of predictive, preventive and personalized medicine (PPPM). We explored (1) the impact of ventilator-delivered MP normalized to well-aerated lung (MP WAL ) on physio-anatomical and clinical responses to NIV in COVID-19-related AHRF and (2) the effect of prone position(PP) on MP WAL . Methods We analyzed 216 noninvasively ventilated COVID-19 patients (108 patients receiving PP + NIV and 108 propensity score-matched patients receiving supine NIV) with moderate-to-severe(paO2/FiO2 ratio < 200) AHRF enrolled in the PRO-NIV controlled non-randomized study (ISRCTN23016116). Quantification of differentially aerated lung volumes by lung ultrasonography (LUS) was validated against CT scans. Respiratory parameters were hourly recorded, ABG were performed 1 h after each postural change. Time-weighed average values of ventilatory variables, including MP WAL , and gas exchange parameters (paO2/FiO2 ratio, dead space indices) were calculated for each ventilatory session. LUS and circulating biomarkers were assessed daily. Results Compared with supine position, PP was associated with a 34% MP WAL reduction, attributable largely to an absolute MP reduction and secondly to an enhanced lung reaeration. Patients receiving a high MP WAL during the 1 st 24 h of NIV [MP WAL (day 1)] had higher 28-d NIV failure (HR = 4.33,95%CI:3.09 − 5.98) and death (HR = 5.17,95%CI: 3.01 − 7.35) risks than those receiving a low MP WAL (day 1). In Cox multivariate analyses, MP WAL (day 1) remained independently associated with 28-d NIV failure (HR = 1.68,95%CI:1.15–2.41) and death (HR = 1.69,95%CI:1.22–2.32). MP WAL (day 1) outperformed other power measures and ventilatory variables as predictor of 28-d NIV failure (AUROC = 0.89;95%CI:0.85–0.93) and death (AUROC = 0.89;95%CI:0.85–0.94). MP WAL (day 1) predicted also gas exchange, ultrasonographic and inflammatory biomarker responses, as markers of VILI, on linear multivariate analysis. Conclusions In the framework of PPPM, early bedside MP WAL calculation may provide added value to predict response to NIV and guide subseque...

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“…Xie Y et al [40], comparó la LPIV en diferentes condiciones de PM, para explorar la participación del receptor de quimiocinas Cxcl10/Cxcr3. Se dividieron en 2 grupos, PM elevada y baja.…”

Section: Pm Lpiv Y Mortalidad Evidencia Clínicaunclassified

Poder mecánico, variable relacionada a la lesión pulmonar inducida por la ventilación y la mortalidad

Cuba-Naranjo¹,

Sosa-Remón²,

Jeréz-Alvarez³

2023

Rev. chil. anest.

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Mechanical power is a variable of interest in lung protection, involving the amount of energy dissipated in the lung parenchyma in each respiratory cycle by means of calculations derived from the respiratory motion equation. The studies consulted associate mechanical power to mortality in patients with acute respiratory distress syndrome. It allows identifying the risk of ventilator-induced damage and pulmonary complications and can be safely used as a marker of improvement in the objectives and goals in artificial mechanical ventilation.

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High Expression of CXCL10/CXCR3 in Ventilator-Induced Lung Injury Caused by High Mechanical Power

Cited by 10 publications

References 30 publications

Whole Transcriptomic Analysis of Key Genes and Signaling Pathways in Endogenous ARDS

Whole Transcriptomic Analysis of Key Genes and Signaling Pathways in Endogenous ARDS

Mechanical power normalized to aerated lung predicts noninvasive ventilation failure and death and contributes to the benefits of proning in COVID-19 hypoxemic respiratory failure

Poder mecánico, variable relacionada a la lesión pulmonar inducida por la ventilación y la mortalidad

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