High expression of miR-105-1 positively correlates with clinical prognosis of hepatocellular carcinoma by targeting oncogene NCOA1

Abstract

Increasing evidence supports that microRNA (miRNA) plays a significant functional role in cancer progression by directly regulating respective targets. In this study, the expression levels of miR-105-1 and its target gene were analyzed using genes microarray and hierarchical clustering analysis followed by validation with quantitative RT-PCR in hepatocellular carcinoma (HCC) and normal liver tissues. We examined the expression of nuclear receptor coactivator 1 (NCOA1), the potential target gene of miR-105-1, following the transfection of miR-105-1 mimics or inhibitors. Our results showed that miR-105-1 was downregulated in HCC tissues when compared with normal liver tissues and patients with lower miR-105-1 expression had shorter overall survival (OS) and progression free survival (PFS). Moreover, NCOA1 was confirmed to be a direct target of miR-105-1. Furthermore, concomitant high expression of NCOA1 and low expression of miR-105-1 correlated with a shorter median OS and PFS in HCC patients. In conclusion, our results provide the first evidence that NCOA1 is a direct target of miR-105-1 suggesting that NCOA1 and miR-105-1 may have potential prognostic value and may be useful as tumor biomarkers for the diagnosis of HCC patients.

MicroRNA-33a downregulation is associated with tumorigenesis and poor prognosis in patients with hepatocellular carcinoma

“…The HCC mortality rate is the fastest growing among all types of cancer and it is the third most common cause of tumor-associated mortality (25)(26)(27). Although there have been improvements in surgery and other therapeutic methods, the 5-year survival rate has remained <15% for a number of years (28)(29)(30)(31).…”

The oncogenic roles of nuclear receptor coactivator 1 in human esophageal carcinoma

“…This suggests that NCOA1 could also serve as a potential biomarker for ESCC prognosis. All these findings are consistent with the report that patients with NCOA1 overexpression along with low expression of miR‐105‐1 were closely associated with both poor overall survival and progression‐free survival in hepatocellular cancer …”

“…Although NCOA1 is the founding member of the p160 family, its functional roles in oncogenesis and progression are not as well‐established as that of SRC‐3, another member of the family with well‐defined oncogenic activity. It has been reported that NCOA1 can promote proliferation of both breast cancer and hepatocellular cancer cells . But results from mice with NCOA1 deletion suggest that NCOA1 is capable of promoting breast cancer metastasis without affecting primary tumor growth, and mechanistically proposed as through NCOA1 mediated crosstalk between tumor cells and their microenvironment .…”

“…All these findings are consistent with the report that patients with NCOA1 overexpression along with low expression of miR-105-1 were closely associated with both poor overall survival and progression-free survival in hepatocellular cancer. 56 Although NCOA1 is the founding member of the p160 family, its functional roles in oncogenesis and progression are not as well-established as that of SRC-3, another member of the family with well-defined oncogenic activity. It has been reported that NCOA1 can promote proliferation of both breast cancer 26 and hepatocellular cancer cells.…”

“…It has been reported that NCOA1 can promote proliferation of both breast cancer 26 and hepatocellular cancer cells. 56 But results from mice with NCOA1 deletion suggest that NCOA1 is capable of promoting breast cancer metastasis without affecting primary tumor growth, and mechanistically proposed as through NCOA1 mediated crosstalk between tumor cells and their microenvironment. 16 We demonstrated that NCOA1 plays essential roles in ESCC cell growth, migration, and metastasis suggesting that NCOA1 is important in each of these processes in ESCC cells.…”