High‐fat diet selectively decreases bone marrow lin ⁻ /CD117 ⁺ cell population in aging mice through increased ROS production

Abstract

Bone marrow (BM) stem cells (BMSCs) are an important source for cell therapy. The outcome of cell therapy could be ultimately associated with the number and function of donor BMSCs. The present study was to evaluate the effect of long-term high-fat diet (HFD) on the population of BMSCs and the role of reactive oxygen species (ROS) in aging mice. Forty-week-old male C57BL/6 mice were fed with HFD for 3 months with regular diet as control. Experiments were repeated when ROS production was reduced in mice treated with N-acetylcysteine (NAC) or using mice overexpressing antioxidant enzyme network (AON) of superoxide dismutase (SOD)1, SOD3, and glutathione peroxidase. BM and blood cells were analyzed with flowcytometry for lineage negative (lin − ) and Sca-1 + , or lin − /CD117 + , or lin − /CD133 + cells. Lin − /CD117 + cell population was significantly decreased with increased intracellular ROS and apoptosis and decreased proliferation in BM, not in blood, in HFDtreated mice without change for Sca-1 + or CD133 + cell populations in BM or blood.NAC treatment or AON overexpression effectively prevented HFD-induced intracellular ROS production and reduction of BM lin − /CD117 + population. These data suggested that long-term HFD selectively decreased BM lin − /CD117 + cell population in aging mice through increased ROS production.

Multiomics profiling reveals VDR as a central regulator of mesenchymal stem cell senescence with a known association with osteoporosis after high-fat diet exposure

“…Additionally, a previous in vivo study revealed that an HFD impairs the proliferative property of BMSCs by increasing ROS levels, which is in line with our findings. 56 Moreover, there was no change in VDR protein expression in BMSCs treated with PA + NAC (Fig. 4d, e ).…”

Effect of Obestatin on Pituitary Gonadal Axis, Leptin and Mda Levels in Obese Sprague Dawley Rats

“…This finding is in accordance with the observations documented by Xaio et al (2020) that the high cholesterol diet induced obesity in male mice resulted in increased MDA levels in obese group when compared to lean rats. The ROS act on the lipid membranes and cause excessive lipid peroxidation resulting in increased levels of MDA 10 .…”

Effect of metabolically divergent pig breeds and tissues on mesenchymal stem cell expression patterns during adipogenesis

“…Previous studies have shown that pre-adipocytes proliferate more rapidly in fat pigs than in lean pigs [ 19 ]. In addition, several studies demonstrated that bone marrow stem cell (BMSCs) from mouse fed a high-fat diet, increased reactive oxygen species (ROS) production [ 20 ] or that obese donors lead to undesirable proliferation, differentiation, and self-renewal of BMSCs [ 21 ]. Our earlier study on undifferentiated synovial mesenchymal stem cells (SMSCs) found breed-specific transcript and signaling pathway variations related to traits like lean stature or obesity in the cell donors [ 12 ].…”