High-Fat Sunflower and Olive Oil Diets Affect Serum Lipid Levels in Steatotic Rat Liver Differently.

Moral, Marı́a Luisa Del; Esteban, Francisco J.; Torres, Marı́a Isabel; Camacho, María Victoria Galloso; Hernández, Raquel; Jiménez, Ana; Aránega, Amelia; Pedrosa, Juan Ángel; Peinado, María Ángeles

doi:10.3177/jnsv.43.155

Cited by 14 publications

(22 citation statements)

References 11 publications

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“…This notion is derived mainly from cohort studies demonstrating the positive impact of the so-called Mediterranean diet on cardiovascular morbidity and mortality (Kris-Etherton 1999). Consistent with our results, previous animal studies have found insulin resistance and elevations of plasma lipids in rats fed high-fat diets based on olive oil (Del Moral et al 1997, Storlien et al 1991, Tsunoda et al 1998. The evidence of specific positive effects of monounsaturated fats on glucose and lipid metabolism is not very conclusive.…”

Section: Discussionsupporting

confidence: 67%

Defining high-fat-diet rat models: metabolic and molecular effects of different fat types

Buettner¹,

Parhofer²,

Woenckhaus³

et al. 2006

Journal of Molecular Endocrinology

597

410

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High-fat (HF)-diet rodent models have contributed significantly to the analysis of the pathophysiology of the insulin resistance syndrome, but their phenotype varies distinctly between different studies. Here, we have systematically compared the metabolic and molecular effects of different HF with varying fatty acid compositions. Male Wistar rats were fed HF diets (42% energy; fat sources: HF-L -lard; HF-O -olive oil; HF-C -coconut fat; HF-F -fish oil). Weight, food intake, whole-body insulin tolerance and plasma parameters of glucose and lipid metabolism were measured during a 12-week diet course. Liver histologies and hepatic gene expression profiles, using Affymetrix GeneChips, were obtained. HF-L and HF-O fed rats showed the most pronounced obesity and insulin resistance; insulin sensitivity in HF-C and HF-F was close to normal. Plasma -3 polyunsaturated fatty acid ( -3-PUFA) and saturated fatty acid (C 12 -C 14 , SFA) levels were elevated in HF-F and HF-C animals respectively. The liver histologies showed hepatic steatosis in HF-L, HF-O and HF-C without major inflammation. Hepatic SREBP1c-dependent genes were upregulated in these diets, whereas PPAR -dependent genes were predominantly upregulated in HF-F fed rats. We detected classical HF effects only in diets based on lard and olive oil (mainly long-chain, saturated (LC-SFA) and monounsaturated fatty acids (MUFA)). PUFA-or MC-SFA-rich diets did not induce insulin resistance. Diets based on LC-SFA and MUFA induced hepatic steatosis with SREBP1c activation. This points to an intact transcriptional hepatic insulin effect despite resistance to insulin's metabolic actions.

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Section: Discussionsupporting

confidence: 67%

Defining high-fat-diet rat models: metabolic and molecular effects of different fat types

Buettner¹,

Parhofer²,

Woenckhaus³

et al. 2006

Journal of Molecular Endocrinology

597

410

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“…Moreover, it is well-known that the type of fat in the diet affects serum lipids and lipoprotein levels, as well as liver morphology and biochemical parameters (8) , and the influence of dietary fat composition on serum lipids and hepatic fibrosis is receiving much attention (9,10). Thus, we have shown that different dietary fats affect serum lipid levels in hepatical steatosis (8).…”

mentioning

confidence: 74%

“…High-fat diets (fat content 14%), semi-purified and balanced , were prepared as previously described (8). The standard (control) diet was a commercially available rat feed (fat content 4-5%).…”

Section: Methodsmentioning

confidence: 99%

“…A laparatomy was performed and blood was collected from the mesenteric vein. Serum lipid measurements and statistical calculations were carried out as described elsewhere (8). Serum low-density lipoprotein-cholesterol (LDL-C) levels were highest in the animals fed the olive-oil diet, in controls as well as in the thioacetamide and dexamethasone-thioacetamide groups.…”

Section: Methodsmentioning

confidence: 99%

“…The possibility of treating acute liver damage with diets of different fat composition, alone or together with dexame thasone, has been evaluated taking into account that high-fat diets showed a steatotic effect by themselves (8).…”

mentioning

confidence: 99%

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Effect of Thioacetamide and Dexamethasone on Serum Lipids in Rats Fed on High-Fat Sunflower or Olive Oil Diets.

Esteban

et al. 1999

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

Self Cite

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SummaryWe have previously reported that high-fat diets develop hepatic steatosis and, depending on the fat quality, affect serum lipid levels differently (J Nutr Sci Vitaminol, 1997, 43,155-160). The aim of this work is to study the influence of high-fat diets (14% sunflower or olive oils) on serum lipids in a model of hepatic acute damage induced by thioacet amide, and their influence when dexamethasone is administered before thioacetamide injection. Serum lipids and hepatic collagen have been evaluated using biochemical methods, and the steatotic process by histological staining. The results showed that hepatic steatosis and fibrosis are developed either by high-fat diets or thioacetamide injection. Pre treatment with dexamethasone did not decrease the hepatic collagen content. Thioacetamide injection alone or pretreatment with dexa methasone produced increases in serum tryglicerides (TG), total cholesterol (TC) and LDL-C in both high-fat diet groups, and a HDL-C increase in the olive-oil group, even though the atherogenic indices (HDL/TC and HDL/TG) were different depending on the enriched diet. The administration of high-fat diets to study the influence of the fat quality on health and disease should be interpreted carefully due to the ability of the diets themselves to cause hepatic damage.

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Dietary Monounsaturated Fat in Early Life Regulates IGFBP2: Implications for Fat Mass Accretion and Insulin Sensitivity

Sabin

Yau

Russo

et al. 2011

Obesity

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articles Pediatric Obesity prospective long-term follow-up (although these are notoriously difficult to undertake), or in animal species where close homology exists with humans. Pigs represent a useful species for the investigation of nutritional effects on later obesity and IS (14), with porcine models of obesity more closely reflecting the metabolic changes seen in human obesity than rodent models (15,16).The primary aim of this study was to investigate the impact of early dietary fat or sugar supplementation on later body composition (BC) and IS in maturing pigs. Methods and ProceduresPorcine study experimental design Ethical approval was granted by the Animal Ethics Committee of the Department of Primary Industries, (Werribee, Australia). Fifty newborn pigs were randomized to receive either a control diet or one supplemented with 18% palm oil (saturated fat (SF)), 18% olive oil (monounsaturated fat: MUF), 9% palm/9% olive oil (mixed fat: MF), or 50% sucrose (SUC) from the time of weaning (day 7). Dietary composition for each of the five diets is shown in Table 1. Within each group, half of the animals were allowed unlimited quantities of food, while the other half were restricted to balance calorie consumption across the diets (~80% of ad libitum intake), in order to ensure that the effect of each diet on IS could be assessed across a broad range of weight gain. Food consumption (g) was measured daily and weight gain (kg) was recorded weekly over a period of 16 weeks (equating to the period of human prepubertal growth).Porcine study end points Body composition. Body composition (BC) was assessed, using whole body dual-energy X-ray absorptiometry (DXA; Hologic QDR4500), at baseline, midstudy and at 16 weeks. Methodology for porcine DXA assessment has been previously described, and appropriate mathematical adjustments were made to determine accurate percentages of lean muscle mass (LMM), fat mass (FM), and bone mass (BM) (17).

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High-Fat Sunflower and Olive Oil Diets Affect Serum Lipid Levels in Steatotic Rat Liver Differently.

Cited by 14 publications

References 11 publications

Defining high-fat-diet rat models: metabolic and molecular effects of different fat types

Defining high-fat-diet rat models: metabolic and molecular effects of different fat types

Effect of Thioacetamide and Dexamethasone on Serum Lipids in Rats Fed on High-Fat Sunflower or Olive Oil Diets.

Dietary Monounsaturated Fat in Early Life Regulates IGFBP2: Implications for Fat Mass Accretion and Insulin Sensitivity

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