High fibroin-loaded silk-PCL electrospun fiber with core–shell morphology promotes epithelialization with accelerated wound healing

Rajasekaran, Ragavi; Dutta, Abir; Ray, Preetam Guha; Seesala, Venkata Sundeep; Ojha, Atul Kumar; Dogra, Nantu; Roy, Sribash; Banerjee, Mamoni; Dhara, Santanu

doi:10.1039/d2tb01890j

Cited by 9 publications

(9 citation statements)

References 39 publications

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“…Moreover, these kinks are more prominent in the wet stent samples indicating delamination among the layers as a result of different extent of swelling of electrospun layers and 3D printed layers. The abovementioned reported mechanical property of the stents was associated with the β sheet formation from the random coil owing to methanol treatment . Notably, experimental data of cervical tissue by Callejas et al, 2020 showed non-linear elastic behavior with differential shear modulus of 1.29 ± 0.15 and 3.60 ± 0.63 MPa for the epithelial layer and connective layer of the cervix, respectively …”

Section: Resultsmentioning

confidence: 95%

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Biodegradable Multi-layered Silk Fibroin-PCL Stent for the Management of Cervical Atresia: In Vitro Cytocompatibility and Extracellular Matrix Remodeling In Vivo

Ojha,

Rajasekaran,

Hansda

et al. 2023

ACS Appl. Mater. Interfaces

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Cervical atresia is a rare congenital Mullerian duct anomaly that manifests as the absence or deformed nonfunctional presence of the cervix. Herein, a multi-layered biodegradable stent is fabricated using a homogeneous blend of silk fibroin with polycaprolactone using hexafluoroisopropanol as a common solution. Briefly, a concentric cylinder of 3D honeycomb layer is sandwiched within electrospun sheets for fixing at the cervico-uterine junction to pave the way of cervical reconstruction. An average length of 40 mm with 3 mm diameter is fabricated for the hybrid stent design. SEM evidences an evenly distributed pore architecture of the electrospun layer, and mechanical characterization of stent reveals a tensile strength of 1.7 ± 0.2 MPa, with a Young's modulus of 5.9 ± 0.1 MPa. Physico-chemical characterization confirms the presence of silk fibroin and poly caprolactone within the engineered stent. Following 14 days of pepsin enzymatic degradation, 18% degradation and a contact angle measurement of 97°are observed. In vitro cytocompatibility studies are performed using site-specific primary human cervical squamous, columnar epithelial cells, and human endometrial stromal cells. The study demonstrates non-cytotoxic cells' viability (no significant toxicity), improved cell anchoring, adherence among the stent layers, and proliferation in the 3D microenvironment. Furthermore, in vivo subcutaneous studies in the rodent model indicate that the implanted stent undergoes constructive remodeling, neo-tissue creation, neo-vasculature formation, and re-epithelialization while maintaining patency for 2 months.

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Section: Resultsmentioning

confidence: 95%

“…The abovementioned reported mechanical property of the stents was associated with the β sheet formation from the random coil owing to methanol treatment. 50…”

Section: Mechanical Propertiesmentioning

confidence: 99%

Biodegradable Multi-layered Silk Fibroin-PCL Stent for the Management of Cervical Atresia: In Vitro Cytocompatibility and Extracellular Matrix Remodeling In Vivo

Ojha,

Rajasekaran,

Hansda

et al. 2023

ACS Appl. Mater. Interfaces

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“…These results indicated that the core–shell fibers can inhibit the burst release phenomenon and effectively prolong the release time of pheromones in the fibers. The ability of core–shell fibers to improve the release behavior of loaded active ingredients has been reported in the field of drug carriers . From the release mechanism of Figure c, unlike TDDA dispersed in monoaxial fibers, the drug-loading system constructed by core–shell fibers concentrated TDDA in the PCL core layer.…”

mentioning

confidence: 90%

“…The ability of core−shell fibers to improve the release behavior of loaded active ingredients has been reported in the field of drug carriers. 48 From the release mechanism of Figure 4c, unlike TDDA dispersed in monoaxial fibers, the drug-loading system constructed by core−shell fibers concentrated TDDA in the PCL core layer. This structure improved the burst release caused by uneven dispersion of TDDA.…”

Section: T H Imentioning

confidence: 99%

Nano-Micro Core–Shell Fibers for Efficient Pest Trapping

Shangguan,

Xu,

Ding

et al. 2023

Nano Lett.

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“…17,22,23 The electrospun nanofiber scaffolds contained sufficient voids to facilitate the exchange of moisture with the external environment, while simultaneously inhibiting bacterial infiltration and effectively reducing the risk of infections. 24,25 The unique structures of electrospun nanofibers allow them to demonstrate novel functional properties. 26−28 Consequently, electrospun nanofiber scaffolds exhibit biological activity and accelerated skin healing.…”

Section: Introductionmentioning

confidence: 99%

Electrospun Nanofiber Scaffold for Skin Tissue Engineering: A Review

Wu,

Yu,

Shao

et al. 2024

ACS Appl. Bio Mater.

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Skin tissue engineering (STE) is widely regarded as an effective approach for skin regeneration. Several synthetic biomaterials utilized for STE have demonstrated favorable fibrillar characteristics, facilitating the regeneration of skin tissue at the site of injury, yet they have exhibited a lack of in situ degradation. Various types of skin regenerative materials, such as hydrogels, nanofiber scaffolds, and 3D-printing composite scaffolds, have recently emerged for use in STE. Electrospun nanofiber scaffolds possess distinct advantages, such as their wide availability, similarity to natural structures, and notable tissue regenerative capabilities, which have garnered the attention of researchers. Hence, electrospun nanofiber scaffolds may serve as innovative biological materials possessing the necessary characteristics and potential for use in tissue engineering. Recent research has demonstrated the potential of electrospun nanofiber scaffolds to facilitate regeneration of skin tissues. Nevertheless, there is a need to enhance the rapid degradation and limited mechanical properties of electrospun nanofiber scaffolds in order to strengthen their effectiveness in soft tissue engineering applications in clinical settings. This Review centers on advanced research into electrospun nanofiber scaffolds, encompassing preparation methods, materials, fundamental research, and preclinical applications in the field of science, technology, and engineering. The existing challenges and prospects of electrospun nanofiber scaffolds in STE are also addressed.

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High fibroin-loaded silk-PCL electrospun fiber with core–shell morphology promotes epithelialization with accelerated wound healing

Abstract: Silk fibroin (SF) is a widely explored biopolymer for wound healing applications due to the presence of amino acids in the biodegradable polymer chain with superior mechanical properties. Herein, a...

Cited by 9 publications

References 39 publications

Biodegradable Multi-layered Silk Fibroin-PCL Stent for the Management of Cervical Atresia: In Vitro Cytocompatibility and Extracellular Matrix Remodeling In Vivo

Biodegradable Multi-layered Silk Fibroin-PCL Stent for the Management of Cervical Atresia: In Vitro Cytocompatibility and Extracellular Matrix Remodeling In Vivo

Nano-Micro Core–Shell Fibers for Efficient Pest Trapping

Electrospun Nanofiber Scaffold for Skin Tissue Engineering: A Review

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