High-fluence low-power laser irradiation promotes odontogenesis and inflammation resolution in periodontitis by enhancing stem cell proliferation and differentiation

Abstract

Periodontitis can exert a severe impact on the life of patients, and the use of stem cell therapy for this disease is promising. The inflammatory response consequent to periodontitis can promote stem cell proliferation. Activated inflammation triggers inhibitory cytokine secretion, thus reducing inflammation subsequent to stem cell activation. High-fluence low-power laser irradiation (HF-LPLI) has the ability to regulate stem cell function through its effect on inflammation. Thus, the aim of the present study was to examine whether HF-LPLI is able to activate stem cells to promote regeneration in periodontitis by promoting inflammation resolution, as well as to evaluate the underlying mechanism of action if an effect is observed. Stem cells were treated with HF-LPLI following inflammation activation. Reverse transcription-quantitative polymerase chain reaction and EdU assay were used to evaluate cell proliferation and differentiation. Flow cytometry and immunofluorescence were also used to detect the ability of HF-LPLI to regulate the surrounding inflammatory environment. Animal models of periodontal disease were treated with stem cells and HF-LPLI, and regeneration was detected by hematoxylin and eosin staining and in vivo imaging. It was observed that HF-LPLI promoted inflammation resolution by reducing the excessive inflammatory response, and finally stimulated stem cell proliferation and differentiation. Furthermore, in vivo results revealed that stem cells treated with HF-LPLI induced bone regeneration. HF-LPLI stimulated stem cell proliferation and differentiation by promoting inflammation resolution subsequent to stem cell activation, providing a new strategy for the clinical treatment of periodontitis.

Resveratrol decreases local inflammatory markers and systemic endotoxin in patients with aggressive periodontitis

“…[14] All participants enrolled in this study for 24-hour pharmacokinetics of RV in plasma. These participants completed 24-hour pharmacokinetics of RV at various dosage at the indicated times (0, 0.2, 0.33, 0.5, 0.67, 1, 1.5, 2, 2.5, 3,4,6,8,12,16,18,20,22 and 24 hours).…”

Exosomes derived from P2X7 receptor gene-modified cells rescue inflammation-compromised periodontal ligament stem cells from dysfunction

“…4,6 Due to incomplete knowledge of the underlying mechanism, [7][8][9] therapeutic approaches that protect PDLSCs from inflammation-induced dysfunction (eg, reduced stemness and aberrant differentiation) following transplantation are still in their infancy. 10,11 In our previous study, we identified the role of P2X7 receptor (P2X7R) in inflammation-compromised osteogenic differentiation of PDLSCs, and most importantly, we found that the cell impairment could be reversed by P2X7R gene modification. 8 This finding suggests that the transplantation of P2X7R gene-modified PDLSCs could lead to an improved therapeutic outcome.…”

“…One reason for the lack of success in clinical practice is that PDLSCs are functionally compromised when they are placed in an inflammatory intrabony periodontal defect 4,6 . Due to incomplete knowledge of the underlying mechanism, 7‐9 therapeutic approaches that protect PDLSCs from inflammation‐induced dysfunction (eg, reduced stemness and aberrant differentiation) following transplantation are still in their infancy 10,11 …”

LncRNA HOXA‐AS2 positively regulates osteogenesis of mesenchymal stem cells through inactivating NF‐κB signalling

“…Inflammatory response is widely implicated in multiple cellular processes, such as proliferation, apoptosis and differentiation . It is reported inflammation is significantly associated with osteogenic differentiation of MSCs.…”

“…[10][11][12] Inflammatory response is widely implicated in multiple cellular processes, such as proliferation, apoptosis and differentiation. [13][14][15][16][17] It is reported inflammation is significantly associated with osteogenic differentiation of MSCs. Furthermore, as is widely accepted that activation of inflammatory response has an inhibitory effect on osteogenesis.…”