“…We demonstrate the application of this assay to a systematically designed library of >1,500 promoter variants in yeast. In this assay, we utilize an approach previously used to interrogate several specific loci (Fatemi et al, 2005;Gal-Yam et al, 2006;Jessen et al, 2006;Kilgore et al, 2007;Small et al, 2014;Stees et al, 2015) or to measure mono-nucleosomes occupancy (as well as DNA methylation) genome-wide (Kelly et al, 2012;Taberlay et al, 2014). The method makes use of a methyltransferase that methylates accessible DNA, followed by bisulfite conversion ''recording'' the methylation status (indicative of the DNA occupancy) into the sequence.…”