High frequency in vitro plantlet regeneration in Solanum trilobatum L., an important ethno-medicinal plant and confirmation of genetic fidelity of R1 plantlets by using ISSR and RAPD markers

Pendli, Sreenu; Rohela, Gulab Khan; Jogam, Phanikanth; Bylla, Prasad; Korra, Rajender; Thammidala, Christopher

doi:10.1007/s42535-019-00069-6

Cited by 19 publications

(4 citation statements)

References 46 publications

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“…2 b) and a maximum of 3.75 ± 0.12 roots per shoot with 5.22 ± 0.32 cm root length was induced on the medium (Table 3 ). Earlier researchers established the beneficial effect of reducing the concentration of MS basal medium on in vitro rooting in Quercus sobur L., Solanum trilobatum and Wrightia tomentosa 47 – 49 . Half strength MS basal medium suited the best for in vitro rooting in regenerated T. coccinea shootlet s .…”

Section: Resultsmentioning

confidence: 99%

Efficient micropropagation of Thunbergia coccinea Wall. and genetic homogeneity assessment through RAPD and ISSR markers

Sultana

Das

Chandra

et al. 2022

Sci Rep

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Thunbergia coccinea Wall. ex D. Don being a rare, ornamental and medicinal plant of India, is needed to propagate for conserving the germplasm and analyzing its phytochemical compounds in the future. A reliable protocol for direct in vitro propagation using nodal shoot meristem of T. coccinea as explant was standardized. The highest number of shoots per explant (22.17 ± 0.54) with maximum shoot length (2.36 ± 0.28) in cm was obtained in Murashige and Skoog (MS) medium supplemented with 9.70 µM of 6-furfurylaminopurine (Kinetin) and 0.053 µM of α-naphthaleneacetic acid (NAA) combination, among all the different plant growth regulators (PGR’s) and concentrations tested. The aforesaid PGR’s combination was optimum for axillary shoot bud induction and multiplication in T. coccinea. The best rooting was observed on the half-strength MS medium fortified with 2.68 µM NAA with the highest number of roots per shoot (3.75 ± 0.12) and maximum length (5.22 ± 0.32) in cm. All the in vitro raised plantlets were acclimatized in sterile sand and soil mixture (1:1) with a survival rate of 70% on earthen pots under greenhouse conditions. PCR-based RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeat) molecular markers were employed to determine the genetic homogeneity amongst the plantlets. Twelve (12) RAPD and nine (9) ISSR primers developed a total of 104 and 91 scorable bands, respectively. The band profiles of micropropagated plantlets were monomorphic to the mother, donor in vivo plant, and similarity values varied from 0.9542–1.000. The dendrogram generated through UPGMA (unweighted pair group method with arithmetic mean) showed 99% similarities amongst all tested plants confirming the genetic uniformity of in vitro raised plants.

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Section: Resultsmentioning

confidence: 99%

Efficient micropropagation of Thunbergia coccinea Wall. and genetic homogeneity assessment through RAPD and ISSR markers

Sultana

Das

Chandra

et al. 2022

Sci Rep

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“…Kumlay and Ercisli, (2015) also observed the highest number of shoots per explant in S. tuberosum in combination with BAP+GA3. In Solanum trilobatum, Sreenu et al (2019) found the highest mean number of shoots differentiated de novo augmented with TDZ+NAA. Similarly, Ya-Long Qin et al (2017) observed the maximum regeneration frequency at 0.5 mg/L BA+1.0 mg/L 2,4-D from S. torvum leaf and stem segments derived callus, as it was observed by Ghan Singh et al (2022a) showing the synergistic effect of both auxin+cytokinin combinations.…”

Section: ) Regeneration From Leaf Derived Callusmentioning

confidence: 89%

A Review on In Vitro Regeneration of Ethnomedicinal Plant Turkey Berry (Solanum torvum Swartz)

Maloth¹,

Marka

Nanna

2023

EJBIO

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In this review, we report on the different plant growth regulators (PGRs) affecting the callus induction, callus-mediated regeneration, organogenesis, somatic embryogenesis from different explants and micropropagation through shoot tip and nodal cultures in Turkey Berry (Solanum torvumSW). The fruits of this plant guard against liver and kidney damage, stop certain cancers, and regulate blood sugar levels. They benefit digestion, the management of gout and menstruation, the treatment of anaemia and diabetes, the prevention of intestinal worms, cardiovascular disorders, and strokes. The protocol developed for the induction of callus can be utilized to isolate pharmaceutically important secondary metabolites in S. torvum, and the regeneration protocols optimized in this study can be used for genetic transformation and conservation of S. torvum, not only as a medicinal plant but also a model system.

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“…These results, as per SCoT, ISSR, and RAPD analyses, suggest that there is apparent variation within the species Corynandra chelidonii. The SCoT, RAPD, and ISSR markers were demonstrated to be not only effective techniques to screen genetic differences but also quick, very consistent, cost-effective and one can investigate the whole genome of the plant randomly (Jogam et al 2020;Pendli et al 2019;Rohela et al 2020). The use of more than one molecular marker to detect the genetic dissimilarity is always useful for better results because every molecular marker has its benefits and drawbacks.…”

Section: Relative Differentiation and Estimate Of Gene Flowmentioning

confidence: 99%

Intraspecific genetic variation inCorynandra chelidonii(Angiosperms: Cleomaceae) as revealed by SCoT, ISSR and RAPD analyses

et al. 2020

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The genetic diversity of two subpopulations of Corynandra chelidonii, one of terrestrial and the other of aquatic environments, was measured with molecular markers, such as start codon targeted (SCoT), inter simple sequence repeats (ISSR), and random amplification of polymorphic DNA (RAPD). The traditional morphological traits such as habitat, habit, leaf morphology, the colour of the sepals and petals, number of stamens, and seed morphology formed the base for their realization as two varieties, C. chelidonii var. pallae and C. chelidonii var. chelidonii. The polymorphism between the two variants was 100% with the primers SCoT-2 and OPA-1 and 4, while maximum polymorphism was detected with ISSR-2, SCoT-3, and OPA-3. The study used, for the first time, more than one molecular marker to assess the genetic variation underscoring the morphological variation in Corynandra chelidonii (L.f.) Cochrane & Iltis. The study justifies the recognition of the two subpopulations of Corynandra chelidonii from aquatic and terrestrial environments as two distinct varieties, C. chelidonii var. pallae (Reddy & Raju) V.S.Raju and C. chelidonii var. chelidonii, respectively, based on the traditional taxonomic evidence.

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High frequency in vitro plantlet regeneration in Solanum trilobatum L., an important ethno-medicinal plant and confirmation of genetic fidelity of R1 plantlets by using ISSR and RAPD markers

Cited by 19 publications

References 46 publications

Efficient micropropagation of Thunbergia coccinea Wall. and genetic homogeneity assessment through RAPD and ISSR markers

Efficient micropropagation of Thunbergia coccinea Wall. and genetic homogeneity assessment through RAPD and ISSR markers

A Review on In Vitro Regeneration of Ethnomedicinal Plant Turkey Berry (Solanum torvum Swartz)

Intraspecific genetic variation inCorynandra chelidonii(Angiosperms: Cleomaceae) as revealed by SCoT, ISSR and RAPD analyses

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