High frequency, long term regeneration of rice from callus culture

Lam

Plant Cell Tiss Organ Cult

1985

Short communicationSomatic embryogenesis and plant regeneration from cultured young inflorescences of Oryza sativa L. (rice) Abstract. Compact caUi initiated from young inflorescences of Oryza sativa L. (rice) on the Linsmaier and Skoog's (LS) medium containing 1 to 2.5 mg/1 of 2,4-dichlorophenoxyacetic acid (2,4-D) were used for regeneration studies. After smooth and compact nodules appeared, these calli were transferred to the regeneration medium containing indole-3-acetic acid (IAA) and either kinetin or 6-benzylaminopurine (BAP). Somatic embryos developed in ten days and were examined by histological studies. Some of the embryos showed scutellum-like structures and a coleoptile-coleorhiza bipolar organization. Regenerated plants had the normal chromosome number of 2n = 24.

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration from cultured young inflorescences of Oryza sativa L. (rice)

Lam

Plant Cell Tiss Organ Cult

1985

Plant Cell Tiss Organ Cult

“…Somatic embryogenesis directly from explants could circumvent these problems. Rice embryo formation has been reported for callus derived from leaves (Wernicke et al 1981), roots (Stickler 1991), immature embryos (Heyser et al 1983), microspores (Genovesi & Magil11982) and young inflorescences (Chen et al 1985). To obtain for rice an efficient and reliable procedure of callus induction and plant regeneration we compared three medium combinations used for the induction of cell-lines and regeneration from protoplasts with media described for callus induction on mature rice embryos and direct regeneration from these (Grimes & Hodges 1990;Li & Murai 1990;Masuda et al 1989;Poonsapaya et al 1989).…”

mentioning

confidence: 99%

Efficient plant regeneration through somatic embryogenesis from callus induced on mature rice embryos (Oryza sativa L.)

Rueb

Leneman

Schilperoort

et al. 1994

103

To obtain a reproducible efficient procedure for regeneration of rice plants through somatic embryogenesis from callus four published methods of callus induction and regeneration were compared. Callus was initiated from mature embryos of the Japonica cultivar Taipei 309 of rice (Oryza sativa L.). The number, mass and morphology of the callus formed on the scutellum were dependent on the medium used. A limited humidity and an optimal aeration of the culture vessels enhanced the frequency of embryogenesis and plant regeneration. A method described by Poonsapaya et al. (1989) was found to be the most efficient and was slightly modified. As a result 98% of the T309 embryos formed callus, of which 63% regenerated into plants. Each callus yielded an average of 6 plants. Plant morphology, fertility and seed set of the regenerants were found to be normal.

Pest Management in Rice

“…Recently, rice somaculture systems have been successfully developed (2,3,18,19,20,21,22,23,24,25,26,27,28,29,30). Many genetic variations have been expressed in plants regenerated from somaculture (2,3,9,14,28,31,32,33).…”

Section: Introductionmentioning

confidence: 98%

Somaclonal Variation for Disease Resistance in Rice (Oryza Sativa L.)

Xie

Rush

Cao

1990

Variation in resistance to rice blast and sheath blight was observed in somaclones regenerated from U. S. long-grain cultivars. Among 2,100 R2 somaclonal lines inoculated and screened for sheath blight resistance, three lines regenerated from the susceptible cultivar Labelle showed a high level of resistance. The sheath blight resistant somaclone SC 86-20001 has good agronomic characteristics when compared to the previously available resistant cultivars. The resistance of the three somaclonal lines to sheath blight was stable after 4 years of testing in the greenhouse and in the field. The inheritance of sheath blight resistance in SC 86-20001-5 was controlled by a single recessive gene. Sheath blight resistance of 86-20001-33 was controlled by two independently inherited recessive genes. Selection among F3 lines from crosses of the above lines with cultivar Lemont gave lines with resistance, good plant type, and high yield potential.