High‐Frequency Mechanostimulation of Cell Adhesion

Kadem, Laith F.; Suana, K. Grace; Holz, Michelle; Wang, Wei; Westerhaus, Hannes; Herges, Rainer; Selhuber‐Unkel, Christine

doi:10.1002/ange.201609483

Cited by 9 publications

(9 citation statements)

References 30 publications

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“…Intriguingly, using a single wavelength of visible light at 530 nm, RGD-coupled push-pull azobenzene configuration allowed an oscillatory switching of isomeric form. 45 This high-frequency ligand oscillation (10 2 to 10 5 Hz) was found to stimulate cell mechanosensing and upregulate the adhesion-associated gene expression.…”

Section: Photonic Control Of Cell Adhesion Release and Patterningmentioning

confidence: 99%

“…Exposure and burial of ligand in polyelectrolyte, 44 polymer brush, 8,42 ligand oscillation, 45 and host-guest interaction-mediated ligand availbility 43 Cell adhesion, 8,42,44 cell adhesion and release, 43 and cell mechanosensing 45…”

Section: Uv-vis Photoisomerizable Materialsmentioning

confidence: 99%

“…Interestingly, azobenzene was modified to carry an electron-withdrawing nitro substituent in one ring and an electron-donating methyl group in the other one. 45 This dipolar substitution pattern induced a red-shift of the π-π* absorption into the visible region to enable a very rapid mechanical oscillation of the azobenzene upon an irradiation with visible light. This configuration of azobenzene, named push-pull azobenzene, was coupled to RGD ligand surrounded by PEG brush on the surface of materials.…”

Section: Photonic Control Of Dynamic Nanobiomedical Engineeringmentioning

confidence: 99%

“…Illumination with visible light at 440 nm switched the azobenzene to the trans configuration to allow the RGD ligand to be available, thereby promoting cell adhesion. Interestingly, azobenzene was modified to carry an electron‐withdrawing nitro substituent in one ring and an electron‐donating methyl group in the other one 45 . This dipolar substitution pattern induced a red‐shift of the π‐π* absorption into the visible region to enable a very rapid mechanical oscillation of the azobenzene upon an irradiation with visible light.…”

Section: Photonic Control Of Dynamic Nanobiomedical Engineeringmentioning

confidence: 99%

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Remote active control of nanoengineered materials for dynamic nanobiomedical engineering

Kim

Choi

Shin

et al. 2020

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Cells dynamically interact with native nanostructured extracellular matrix at a molecular level in vivo. Developing remotely and actively controllable nanoengineered biomaterials can manipulate and unravel complex cell-material interactions that dynamically occur in the nanoscale in vivo. In this review, we discuss emerging advances in a myriad of recent nanoengineering technologies to design remotely manipulable materials that enable dynamic nanobiomedical engineering at the molecular level. In particular, we focus on remote active stimuli, such as magnetic fields, light, in situ self-assembly, and ultrasound, to manipulate dynamic cell-material interactions in both in vitro and in vivo settings. Remote active control can be particularly appealing with targeting capability for particular locations at any prescribed time points with a degree of reversibility. The unique remote controllability enables the regulation of cellular signaling, adhesion, differentiation, and polarization; cell, drug, and gene delivery; and in situ self-assembly. These materials allow the remote control in regenerative medicine, immunotherapy, cancer therapy, and biocatalysis as well as mechanistic studies on dynamic nanoscale cell-material interactions. We also highlight current challenges in the remote active control, such as reproducibility, tissue-penetrative capability, noninvasive surgery, spatial localization, and temporal variation. Albeit remotely and actively controllable nanoengineered biomaterials are in the nascent stage of development, they can evolve into multiresponsive, reversible, and cost-effective three-dimensional systems with safe and convenient long-term control at the cell, tissue, and organ level toward clinical patient-tailorable on-demand therapy. K E Y W O R D S dynamic nanobiomedical engineering, magnetic control, nanoengineered biomaterial, photonic control, remote active control, self-assembly-based control This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Section: Photonic Control Of Cell Adhesion Release and Patterningmentioning

confidence: 99%

Section: Uv-vis Photoisomerizable Materialsmentioning

confidence: 99%

Section: Photonic Control Of Dynamic Nanobiomedical Engineeringmentioning

confidence: 99%

Section: Photonic Control Of Dynamic Nanobiomedical Engineeringmentioning

confidence: 99%

See 2 more Smart Citations

Remote active control of nanoengineered materials for dynamic nanobiomedical engineering

Kim

Choi

Shin

et al. 2020

VIEW

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“…Much of our understanding of the molecular mechanisms underlying force-stimulated cellular processes has been garnered from studies using micropipettes 21 , atomic force microscopes (AFM) 22 , or optical tweezers 23 to apply forces to cells at subcellular level. The quantification of the applied forces, in parallel with the imaging of cell responses, have identified major players and force ranges in the mechanotransduction chain 24 .…”

Section: Mechanical Stimulation On the T Cell Receptor (Tcr) Leads Tomentioning

confidence: 99%

Optoregulated force application to cellular receptors using molecular motors

Zheng

Han

Zhao

et al. 2020

Preprint

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Mechanotransduction events in physiological environments are difficult to investigate, in part due to the lack of experimental tools to apply forces to mechanosensitive receptors remotely. Inspired by cellular mechanisms for force application (i.e. motor proteins pulling on cytoskeletal fibers), here we present a unique molecular machine that can apply forces at cell-matrix and cell-cell junctions using light as an energy source. The key actuator is a light-driven rotatory molecular motor linked to polymer chains, which is intercalated between a membrane receptor and an engineered biointerface. The lightdriven actuation of the molecular motor is converted in mechanical twisting of the polymer chains, which will in turn effectively "pulls" on engaged cell membrane receptors (integrins, cadherins…) within the illuminated area. Applied forces have the adequate magnitude and occur at time scales within the relevant ranges for mechanotransduction at cell-friendly exposure conditions, as demonstrated in forcedependent focal adhesion maturation and T cell activation experiments. Our results reveal the potential of nanomotors for the manipulation of living cells at the molecular scale and demonstrate, for the first time, a functionality which at the moment cannot be achieved by any other means.

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Photo‐ECM: A Blue Light Photoswitchable Synthetic Extracellular Matrix Protein for Reversible Control over Cell–Matrix Adhesion

2019

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The dynamic and spatiotemporal control of integrin‐mediated cell adhesion to RGD motifs in its extracellular matrix (ECM) is important for understating cell biology and biomedical applications because cell adhesion fundamentally regulates cellular behavior. Herein, the first photoswitchable synthetic ECM protein, Photo‐ECM, based on the blue light switchable protein LOV2 is engineered. The Photo‐ECM protein includes a RGD sequence, which is hidden in the folded LOV2 protein structure in the dark and is exposed under blue light so that integrins can bind and cells can adhere. The switchable presentation of the RGD motif allows to reversibly mediate and modulate integrin‐based cell adhesions using noninvasive blue light. With this protein cell adhesions in live cells could be reversed and the dynamics at the cellular level is observed. Hence, the Photo‐ECM opens a new possibility to investigate the spatiotemporal regulation of cell adhesions in cell biology and is the first step toward a genetically encoded and light‐responsive ECM.

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High‐Frequency Mechanostimulation of Cell Adhesion

Cited by 9 publications

References 30 publications

Remote active control of nanoengineered materials for dynamic nanobiomedical engineering

Remote active control of nanoengineered materials for dynamic nanobiomedical engineering

Optoregulated force application to cellular receptors using molecular motors

Photo‐ECM: A Blue Light Photoswitchable Synthetic Extracellular Matrix Protein for Reversible Control over Cell–Matrix Adhesion

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