High Frequency of Aneuploidy Defines Ulcerative Colitis-Associated Carcinomas

Gerling, Marco; Meyer, K. F.; Fuchs, Katrin; Igl, Bernd W.; Fritzsche, Britta; Ziegler, Andreas; Bader, Franz G.; Kujath, P.; Schimmelpenning, H.; Bruch, Hans–Peter; Roblick, Uwe J.; Habermann, Jens K.

doi:10.1097/sla.0b013e3181deb664

Cited by 31 publications

(35 citation statements)

References 29 publications

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“…The prognostic potential of DNA ploidy was shown in a study involving 376 endometrial carcinoma patients with a more favorable 5-year survival rate of 94% for patients with diploid cell populations as opposed to those with aneuploid malignancies (83%) [4]. DNA ploidy can be assessed by flow or image cytometry [5], the latter case assessing cells on tissue sections [6]. We demonstrated for breast carcinomas that not only the ploidy status per se but also the grade of genomic instability has an impact on patient prognosis [7].…”

Section: Introductionmentioning

confidence: 99%

Protein profiling of genomic instability in endometrial cancer

Gemoll

Habermann

Lahmann

et al. 2011

Cell. Mol. Life Sci.

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DNA aneuploidy has been identified as a prognostic factor in the majority of epithelial malignancies. We aimed at identifying ploidy-associated protein expression in endometrial cancer of different prognostic subgroups. Comparison of gel electrophoresis-based protein expression patterns between normal endometrium (n = 5), diploid (n = 7), and aneuploid (n = 7) endometrial carcinoma detected 121 ploidy-associated protein forms, 42 differentially expressed between normal endometrium and diploid endometrioid carcinomas, 37 between diploid and aneuploid endometrioid carcinomas, and 41 between diploid endometrioid and aneuploid uterine papillary serous cancer. Proteins were identified by mass spectrometry and evaluated by Ingenuity Pathway Analysis. Targets were confirmed by liquid chromatography/mass spectrometry. Mass spectrometry identified 41 distinct polypeptides and pathway analysis resulted in high-ranked networks with vimentin and Nf-κB as central nodes. These results identify ploidy-associated protein expression differences that overrule histopathology-associated expression differences and emphasize particular protein networks in genomic stability of endometrial cancer.

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Section: Introductionmentioning

confidence: 99%

Protein profiling of genomic instability in endometrial cancer

Gemoll

Habermann

Lahmann

et al. 2011

Cell. Mol. Life Sci.

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“…In line, an assessment of the gross nuclear ploidy status in 257 sporadic colorectal carcinomas identified aneuploidy (OR 4.07; 95% CI 1.46-11.36; p = 0.007) and higher age at diagnosis (OR 1.05; 95% CI 1.02-1.09; p = 0.003) as independent prognostic parameters [59]. Thus, for R0-resected colorectal cancer patients, DNA aneuploidy was the strongest independent prognostic marker regarding postoperative 5-year survival.…”

Section: Colon Cancer Tissue and Serum Diagnosticsmentioning

confidence: 93%

Applying Genomics and Proteomics in Translational Surgical Oncology Research

Gemoll

Meyer

Habermann³

2015

Eur Surg Res

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Background: Translational surgical research can range from investigating clinically oriented basic pathophysiological research via the correlation of molecular findings with a patient's clinical outcome to conducting treatment response studies. Herein, the specialist's opinion as a ‘surgeon scientist' plays a pivotal role, e.g. in planning experimental setups focusing on the clinically most relevant needs. Summary and Key Messages: This review provides a survey of genomic and proteomic technologies and gives an up-to-date overview of those studies applying these technologies exemplarily in colorectal cancer-based biomarker research. Although current results are promising, future validation studies within multicenter networks are required to promote the translation of ‘omics' from bench to bedside. In this endeavor, departments of surgery and institutes of experimental surgery together should play a fundamental role. The program for ‘clinician scientists' recently launched by the German Research Society (DFG) could be one tool to promote interdisciplinary networks and surgeons' impact on translational research.

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“…Histograms were classified according to Auer (Figure 2) (Auer, Caspersson, and Wallgren 1980). UCCs presented aneuploidy at significantly higher frequency than sporadic colorectal carcinomas (100% versus 74.6%; P < 0.0006) (Gerling et al 2010). In addition, we performed a logistic regression analysis comprising age, sex, UICC stage, T-and N-status, histologic tumor grading, underlying inflammation, and DNA ploidy status.…”

Section: Nuclear Aneuploidy and Prognosis In Sporadic And Uc-associatmentioning

confidence: 99%

“…This was further supported by Kaplan-Meier-Plots showing that diploid SCCs had a more favorable 5-year survival (88.2%) than aneuploid SCCs (69.0%) and UCCs (73.1%) (P = 0.074). Thus, aneuploidy proved to be the strongest independent prognostic marker for R0-resected colorectal cancer patients overall (Gerling et al 2010). Fig.…”

Section: Nuclear Aneuploidy and Prognosis In Sporadic And Uc-associatmentioning

confidence: 99%