2002
DOI: 10.1053/jhep.2002.30275
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High frequency of epithelial chimerism in liver transplants demonstrated by microdissection and STR-analysis

Abstract: It has recently been shown that epithelial cells derived from stem cells originating outside the liver are integrated into liver allografts. Whether epithelial intragraft chimerism protects transplants from rejection or chronic transplant dysfunction, and whether it interferes with recurrence of primary liver disease, is not known. Twenty-seven sequential biopsies derived from 9 liver-transplant recipients were studied for chimerism of hepatocytes and cholangiocytes. The target cells were isolated by laser mic… Show more

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Cited by 105 publications
(88 citation statements)
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“…10 -12 Generation of hepatocytes from bone marrow cells also occurs after allergenic liver transplantation in humans and is associated with the frequency and severity of rejection episodes. 37 The molecular events, however, inducing differentiation and transdifferentiation in adult and embryonic/fetal stem cells are primarily unknown. Stage-and differentiation-specific gene expression patterns with quantitative PCR and genome-wide technologies may be helpful in unraveling the underlying molecular mechanisms and will be crucial for developing in vitro differentiation protocols for stem cell populations.…”
Section: Discussionmentioning
confidence: 99%
“…10 -12 Generation of hepatocytes from bone marrow cells also occurs after allergenic liver transplantation in humans and is associated with the frequency and severity of rejection episodes. 37 The molecular events, however, inducing differentiation and transdifferentiation in adult and embryonic/fetal stem cells are primarily unknown. Stage-and differentiation-specific gene expression patterns with quantitative PCR and genome-wide technologies may be helpful in unraveling the underlying molecular mechanisms and will be crucial for developing in vitro differentiation protocols for stem cell populations.…”
Section: Discussionmentioning
confidence: 99%
“…Fragments of laser-destroyed cells do not cause contamination of STR-PCR as shown previously. 5,6 Chimerism-negative tumors could be taken as controls that indicate elimination of the contamination problem by the methodological approach applied. Furthermore, we confirmed our STR-PCR results by X chromosome in situ hybridization revealing tumor cells with a XX karyotype in allografts from male donors in a female recipient.…”
Section: Discussionmentioning
confidence: 99%
“…[5][6][7][8]13 To exclude any contamination of the following PCR analysis by infiltrating recipient cells, an immunohistochemical stain for leukocytes and histiocytes combining antibodies against CD45 and CD68 (both DAKO, Hamburg, Germany) was performed following a standard ABC technique. 8 Laser-based microdissection of pure epithelial tumor cell populations were performed using the PALM Laser MicroBeam system (P.A.L.M., Bernried, Germany).…”
Section: Laser Capture Microdissection and Dna Preparationmentioning
confidence: 99%
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