High frequency plant regeneration following abnormal shoot organogenesis in the medicinal tree Hovenia dulcis

Abstract

An efficient plant regeneration protocol for shoot organogenesis from Hovenia dulcis callus cultures was established. Induction of organogenic callus was achieved on Murashige and Skoog (MS) medium supplemented with 4.65 lM kinetin and 4.5 lM 2,4-dichlorophenoxyacetic acid (2,4-D). Further differentiation of organogenic callus into primordia, shoot-like structures, and plantlets was achieved on MS medium supplemented with 0.23 lM gibberellic acid (GA 3 ) and 0.46 lM kinetin. Numerous abnormal shoots developed upon transfer of callus to MS medium containing cytokinins, and these failed to grow further into whole plantlets. However, transfer of 'abnormal' shoots to a fresh MS medium lacking cytokinins resulted in growth of normal shoots. Elongated shoots subsequently were rooted in basal MS medium, and whole plantlets were established in a soil mix. Analysis of regenerated plants using random amplified polymorphic DNA (RAPD) confirmed the genetic stability of these regenerant plantlets.

Compact callus cultures and evaluation of the antioxidant activity of Hovenia dulcis Thunb. (Rhamnaceae) under in vivo and in vitro culture conditions

“…In the present study, friable calli were observed when 2,4-D and PIC were added to the culture medium, while only NAA induced compact callus which is in contrast with Jeong et al (2009) findings, where 2,4-D promoted the formation of compact callus in leaf explants. Similar results were reported for other species such as Gossypium hirsutum, where 2,4-D promoted only friable callus while NAA was responsible for compact callus formation (Baksha et al, 2006).…”

<i>In Vitro</i> Organogenesis of <i>Quisqualis indica </i>Linn.

“…In the present investigation, it was observed that the total number of regenerants increased as the concentrations of TDZ increased in combination with 0.5 mg/L GA 3 or 0.25 mg/L NAA. This was in line with the observations on differentiation of organogenic callus into plantlets in Hovenia dulcis [33] on MS medium supplemented with 0.23 µM gibberellic acid and 0.46µM kinetin. Similarly maximum number of shoots was achieved from leaf section callus of Hyptis suaveolens [34] on MS medium containing 0.5 mg/L BA and 0.5 mg/L GA 3 .…”

Efficient micropropagation and assessment of genetic fidelity of Boerhaavia diffusa L- High trade medicinal plant

“…Molecular marker techniques, such as random amplified polymorphic DNA (RAPD) analysis, have been used to assess variability or similarity within or between the plants derived from plant tissue culture and the donor plant (Jeong et al 2009;Jain et al 2011). RAPD is a simple, largely automatable technique which requires only a small amount of DNA and can be performed without the use of radioactivity (Williams et al 1990).…”