1993
DOI: 10.1073/pnas.90.3.913
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High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene.

Abstract: We report here a 100-fold increased frequency of plastid transformation in tobacco by selection for a chimeric aadA gene encoding aminoglycoside 3"-adenylyltransferase, as compared with that obtained with mutant 16S rRNA genes. Expression of aad4 confers resistance to spectinomycin and streptomycin. In transforming plasmid pZS197, a chimeric aadA is cloned between rbcL and open reading frame ORF512 plastid gene sequences. Selection was for spectinomycin resistance after biolistic delivery of pZS197 DNA into le… Show more

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Cited by 707 publications
(835 citation statements)
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“…They derive from pZS197 vector (Svab and Maliga 1993) and correspond to vectors described by Newell et al (2003). The aadA selectable marker gene is under the control of the rrn promoter, the rbcL 5 0 -UTR and psbA 3 0 -UTR sequences.…”
Section: Plastid Transformation Vectorsmentioning
confidence: 99%
“…They derive from pZS197 vector (Svab and Maliga 1993) and correspond to vectors described by Newell et al (2003). The aadA selectable marker gene is under the control of the rrn promoter, the rbcL 5 0 -UTR and psbA 3 0 -UTR sequences.…”
Section: Plastid Transformation Vectorsmentioning
confidence: 99%
“…The 100% resistance to spectinomycin in all of the clones examined shows maternal inheritance of the introduced genes. Lack of pollen transmission of the aadA gene in chloroplast transgenic plants is expected in tobacco, a species with strict maternal inheritance of its plastids 16 . A heteroplasmic condition would have given rise to variegated progeny on spectinomycin 16,20 ; lack of such variegated progeny also confirms homoplasmy as shown by Southern blot analysis (Fig.…”
Section: Characterization Of Transgenic Plantsmentioning
confidence: 99%
“…The discovery of the gene gun as a transformation device opened the possibility of direct plastid transformation 12 . Transient expression of foreign genes in plastids of dicots 13,14 and monocots 15 , prolonged foreign gene expression using autonomously replicating chloroplast expression vectors 13 , and stable integration of a selectable marker into the tobacco chloroplast genome 16 were accomplished using the gene gun. Tobacco plants resistant to certain insects were obtained by integrating the cryIAc gene into the tobacco chloroplast genome 17 .…”
mentioning
confidence: 99%
“…1a) were introduced into tobacco (Nicotiana tabacum cv. Petit Havana) plastids by biolistic transformation (Svab and Maliga 1993). Primary spectinomycin-resistant tobacco lines were selected on regeneration medium of plants (RMOP) containing 500 mg/L spectinomycin.…”
Section: Introduction Of Transgenes Into the Tobacco Plastid Genomementioning
confidence: 99%