High frequency repetitive Transcranial Magnetic Stimulation promotes long lasting phrenic motoneuron excitability via GABAergic networks

Michel‐Flutot, Pauline; Zholudeva, Lyandysha V.; Randelman, Margo L.; Deramaudt, Thérèse B.; Mansart, Arnaud; Alvarez, Jean‐Claude; Lee, Kun‐Ze; Petitjean, Michel; Bonay, Marcel; Lane, Megan; Vinit, Stéphane

doi:10.1016/j.resp.2021.103704

Cited by 11 publications

(10 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present study is the first to investigate the therapeutic effects of chronic rTMS for enhancing respiratory function following cervical spinal cord injury (SCI) in a preclinical model. Our previous study already demonstrated that a single acute delivery of 10 Hz rTMS in anesthetized rats induced an increase in phrenic network excitability [30]. This 10 Hz magnetic stimulation is recognized for its long-term potentiation (LTP)-like effect [42,52].…”

Section: Discussionmentioning

confidence: 97%

“…The protocol (9 trains of 100 biphasic pulses, separated by 30 s intervals between trains delivered at 50% MO, 900 stimulations per protocol) was applied in awake restrained animals at -6 mm caudal to Bregma. This protocol induced a long-lasting increase in phrenic excitability in anesthetized, intact rats [30]. Control animals received a Sham rTMS protocol (e.g., no stimulation but the same time spent in the custom-designed restraining device, Figure 1).…”

Section: Repetitive Tms (Rtms) Protocolmentioning

confidence: 99%

“…Next, the head of the animal was placed on a nonmagnetic, custom-made stereotaxic apparatus, which allowed its positioning from the center of the figure-of-eight coil to −6 mm from Bregma, at an angle of 0 • , as previously described [30,31]. MEPdia induced by a single pulse of TMS was recorded (summation of 5 to 10 TMS pulses between 2 heartbeats, max 10 trials at 90% MO).…”

Section: Diaphragmatic Mep Recordingsmentioning

confidence: 99%

“…We recently demonstrated that a single train of TMS delivered above the animals' motor threshold can induce a long-lasting increase in phrenic system excitability, as measured with diaphragm motor evoked potentials (MEPdia) [30]. While this study was focused on determining stimulation parameters in naïve, anesthetized rats, it demonstrated that MEPdia can be used as a reliable and reproducible technique for assessing phrenic system excitability during TMS [31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of Chronic High-Frequency rTMS Protocol on Respiratory Neuroplasticity Following C2 Spinal Cord Hemisection in Rats

Michel‐Flutot

Jesus

Vanhee

et al. 2022

Biology

Self Cite

View full text Add to dashboard Cite

High spinal cord injuries (SCIs) lead to permanent diaphragmatic paralysis. The search for therapeutics to induce functional motor recovery is essential. One promising noninvasive therapeutic tool that could harness plasticity in a spared descending respiratory circuit is repetitive transcranial magnetic stimulation (rTMS). Here, we tested the effect of chronic high-frequency (10 Hz) rTMS above the cortical areas in C2 hemisected rats when applied for 7 days, 1 month, or 2 months. An increase in intact hemidiaphragm electromyogram (EMG) activity and excitability (diaphragm motor evoked potentials) was observed after 1 month of rTMS application. Interestingly, despite no real functional effects of rTMS treatment on the injured hemidiaphragm activity during eupnea, 2 months of rTMS treatment strengthened the existing crossed phrenic pathways, allowing the injured hemidiaphragm to increase its activity during the respiratory challenge (i.e., asphyxia). This effect could be explained by a strengthening of respiratory descending fibers in the ventrolateral funiculi (an increase in GAP-43 positive fibers), sustained by a reduction in inflammation in the C1–C3 spinal cord (reduction in CD68 and Iba1 labeling), and acceleration of intracellular plasticity processes in phrenic motoneurons after chronic rTMS treatment. These results suggest that chronic high-frequency rTMS can ameliorate respiratory dysfunction and elicit neuronal plasticity with a reduction in deleterious post-traumatic inflammatory processes in the cervical spinal cord post-SCI. Thus, this therapeutic tool could be adopted and/or combined with other therapeutic interventions in order to further enhance beneficial outcomes.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Repetitive Tms (Rtms) Protocolmentioning

confidence: 99%

Section: Diaphragmatic Mep Recordingsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of Chronic High-Frequency rTMS Protocol on Respiratory Neuroplasticity Following C2 Spinal Cord Hemisection in Rats

Michel‐Flutot

Jesus

Vanhee

et al. 2022

Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Gamma-aminobutyric acid (GABA) is an inhibitory neurotransmitter because it inhibits certain interneuronal synaptic signals, thereby preventing or reducing the risk of CNP ( Gwak and Hulsebosch, 2011 ). Some studies found that single rTMS protocol increased phrenic motoneuron excitability at 10 Hz through the mediation of a local GABA ergic disinhibition ( Barr et al, 2013 ; Michel-Flutot et al, 2021 ). rTMS (10 Hz) alleviated acute CNP in the early stages of SCI by improving motor-evoked potential (MEP) parameters and modulating BDNF and nerve growth factor (NGF) secretion ( Zhao et al, 2020 ).…”

Section: Repetitive Transcranial Magnetic Stimulation For Central Neu...mentioning

confidence: 99%

Non-invasive Brain Stimulation for Central Neuropathic Pain

Yang

Zhang

et al. 2022

Front. Mol. Neurosci.

View full text Add to dashboard Cite

The research and clinical application of the noninvasive brain stimulation (NIBS) technique in the treatment of neuropathic pain (NP) are increasing. In this review article, we outline the effectiveness and limitations of the NIBS approach in treating common central neuropathic pain (CNP). This article summarizes the research progress of NIBS in the treatment of different CNPs and describes the effects and mechanisms of these methods on different CNPs. Repetitive transcranial magnetic stimulation (rTMS) analgesic research has been relatively mature and applied to a variety of CNP treatments. But the optimal stimulation targets, stimulation intensity, and stimulation time of transcranial direct current stimulation (tDCS) for each type of CNP are still difficult to identify. The analgesic mechanism of rTMS is similar to that of tDCS, both of which change cortical excitability and synaptic plasticity, regulate the release of related neurotransmitters and affect the structural and functional connections of brain regions associated with pain processing and regulation. Some deficiencies are found in current NIBS relevant studies, such as small sample size, difficulty to avoid placebo effect, and insufficient research on analgesia mechanism. Future research should gradually carry out large-scale, multicenter studies to test the stability and reliability of the analgesic effects of NIBS.

show abstract

Repetitive Transcranial Magnetic Stimulation-Mediated Neuroprotection in the 5xFAD Mouse Model of Alzheimer’s Disease Through GABRG2 and SNAP25 Modulation

Wang,

Zhou,

Huang

et al. 2024

Mol Neurobiol

View full text Add to dashboard Cite

High frequency repetitive Transcranial Magnetic Stimulation promotes long lasting phrenic motoneuron excitability via GABAergic networks

Cited by 11 publications

References 64 publications

Effects of Chronic High-Frequency rTMS Protocol on Respiratory Neuroplasticity Following C2 Spinal Cord Hemisection in Rats

Effects of Chronic High-Frequency rTMS Protocol on Respiratory Neuroplasticity Following C2 Spinal Cord Hemisection in Rats

Non-invasive Brain Stimulation for Central Neuropathic Pain

Repetitive Transcranial Magnetic Stimulation-Mediated Neuroprotection in the 5xFAD Mouse Model of Alzheimer’s Disease Through GABRG2 and SNAP25 Modulation

Contact Info

Product

Resources

About